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The map label for this gene is recJ [H]

Identifier: 218691017

GI number: 218691017

Start: 3301885

End: 3303618

Strand: Reverse

Name: recJ [H]

Synonym: ECED1_3351

Alternate gene names: 218691017

Gene position: 3303618-3301885 (Counterclockwise)

Preceding gene: 218691018

Following gene: 218691015

Centisome position: 63.41

GC content: 56.34

Gene sequence:

>1734_bases
GTGAAACAACAGATACAACTTCGTCGCCGAGAAGTCGATGAAACGGCCGACTTGCCCGCTGAATTGCCTCCCTTGCTGCG
CCGTTTATATGCCAGCCGGGGTGTGCGTAGCGCGCAGGAACTGGAACGCAGTGTTAAAGGCATGCTGCCCTGGCAGCAAC
TGAGCGGCGTCGAAAAGGCCGTTGAGATCCTTTACAACGCTTTTCGCGAAGGAACGCGGATTATTGTGGTCGGTGATTTC
GACGCCGACGGTGCGACCAGCACGGCTCTAAGCGTGCTGGCGATGCGCTCGCTTGGTTGCAGCAATATCGACTACCTGGT
TCCAAACCGTTTCGAAGACGGTTACGGCTTAAGCCCGGAAGTAGTCGATCAGGCTCATGCCCGTGGCGCGCAGTTAATTG
TCACGGTGGATAACGGTATTTCCTCCCATGCGGGGGTTGAACACGCTCGTTCGTTGGGCATTCCGGTTATTGTTACCGAT
CACCATTTGCCAGGCGACACATTACCCGCAGCGGAAGCGATCATTAACCCTAACTTGCGCGACTGTAATTTCCCGTCGAA
ATCACTGGCAGGCGTGGGTGTGGCGTTTTATCTGATGCTGGCGCTGCGCACCTTTTTGCGCGATCAGGGCTGGTTTGATG
AGCGCGGCATCGCAATTCCTAATCTGGCAGAACTGCTGGATCTGGTAGCGTTGGGGACAGTGGCGGACGTCGTGCCGCTG
GACGCTAATAATCGCATTCTGACCTGGCAGGGGATGAGTCGCATCCGTGCCGGAAAGTGCCGTCCGGGGATTAAAGCGCT
GCTGGAAGTGGCCAATCGCGATCCACAAAAACTCGCCGCCAGCGATTTAGGTTTTGCGCTGGGGCCACGTCTCAATGCTG
CCGGGCGACTGGACGATATGTCCGTTGGCGTGGCGCTGTTGTTGTGTGACAACATTGGCGAAGCGCGTGTGCTGGCGAAT
GAACTCGATGCACTAAACCAAACACGTAAAGAGATCGAACAGGGAATGCAGGTTGAAGCGCTCACTCTGTGCGAGAAGCT
GGAACGTAGCCGCGACACGCTGCCAGGTGGGCTGGCAATGTATCACCCTGAATGGCATCAGGGCGTTGTTGGCATTCTGG
CATCGCGTATCAAAGAGCGTTTTCACCGTCCGGTTATCGCCTTTGCGCCTGCGGGCGACGGTACGCTGAAAGGTTCCGGT
CGCTCCATTCAGGGGCTGCATATGCGCGATGCGCTGGAGCGGCTAGACACGCTTTACCCCGGCATGATGCTCAAGTTTGG
CGGCCATGCGATGGCGGCAGGATTGTCGCTGGAAGAGGATAAATTCGAACTCTTTCAGCAACGCTTTGGCGAACTGGTTA
CCGAGTGGTTAGATCCTTCTTTATTGCAAGGGGAAGTGGTATCAGACGGCCCATTAAGTGCGGCAGAGATGACCATGGAA
GTGGCGCAGCTGTTGCGTGATGCTGGCCCGTGGGGGCAGATGTTCCCGGAACCGTTGTTTGACGGTCATTTCCGTCTGTT
GCAACAGCGGCTGGTGGGCGAGCGTCATTTGAAGGTGATGGTCGAACCGGTCGGCGGCGGTCCACTGCTGGACGGGATTG
CTTTTAATGTCGATACCGCCCTCTGGCCGGATAACGGTGTGCGCGAAGTGCAACTGGCTTACAAGCTCGATATCAACGAG
TTTCGCGGCAACCGCAGCCTGCAAATTATCATCGACAATATCTGGCCAATTTAG

Upstream 100 bases:

>100_bases
TGCTGAGCAATGGCACACTTGTTCCGGGTTACCAGCCGCCGAAAGACATGAAAGAATTCCTCGACGAACACCAAAAAATG
ACCAGCGGTAAATAATTCGC

Downstream 100 bases:

>100_bases
CGTCATCTTCTCTATAAAAAAGAGCGTGGATTGGGTACAATCCCGCTCTTATCACCGCATTTTGACTAGCTCAATAAAAG
AAATCAGACCATGTTTGAAA

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSAAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Sequences:

>Translated_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSAAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI
>Mature_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSAAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=577, Percent_Identity=99.1334488734835, Blast_Score=1158, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63320; Mature: 63320

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGDTLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSAAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGDTLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSAAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]