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The map label for this gene is ptsP [H]

Identifier: 218690955

GI number: 218690955

Start: 3228961

End: 3231207

Strand: Reverse

Name: ptsP [H]

Synonym: ECED1_3285

Alternate gene names: 218690955

Gene position: 3231207-3228961 (Counterclockwise)

Preceding gene: 218690956

Following gene: 218690954

Centisome position: 62.02

GC content: 53.58

Gene sequence:

>2247_bases
ATGCTCACTCGCCTGCGCGAAATAGTCGAAAAGGTAGCCAGCGCACCACGCCTGAATGAGGCGTTAAATATTCTGGTTAC
CGACATCTGTCTTGCGATGGATACCGAGGTCTGTTCGGTCTACCTAGCCGATCATGATCGACGTTGTTACTACCTGATGG
CGACCCGGGGACTGAAAAAACCACGCGGTCGCACTGTAACGCTCGCGTTTGATGAAGGGATCGTCGGCCTGGTTGGCAGG
CTGGCGGAACCGATAAACCTTGCAGATGCGCAAAAGCACCCCAGCTTCAAATACATCCCCTCCGTAAAAGAAGAACGTTT
CCGCGCGTTTTTAGGCGTACCAATTATTCAACGTCGCCAGTTGCTTGGTGTACTGGTGGTACAGCAACGAGAGTTGCGCC
AGTATGACGAAAGTGAAGAATCCTTCCTGGTGACGCTTGCCACCCAGATGGCAGTTATTCTTTCTCAGTCGCAGTTGACT
GCCTTGTTTGGGCAATATCGCCAGACGCGAATCCGTGCATTACCGGCAGCACCTGGTGTGGCGATTGCCGAAGGCTGGCA
GGATGCCACGTTACCTTTAATGGAACAGGTGTATCAGGCATCAACGCTGGATCCGGCTCTGGAACGCGAACGACTGACCG
GGGCGTTGGAAGAGGCGGCTAACGAGTTTCGCCGCTATAGCAAACGCTTTGCTGCCGGTGCACAAAAAGAAACGGCGGCT
ATTTTCGATCTTTACTCACACCTGCTTTCGGATACCCGGCTGCGTCGCGAATTGTTTGCCGAGGTTGATAAAGGCTCGGT
GGCAGAGTGGGCGGTAAAAACGGTCATTGAAAAATTTGCCGAACAGTTTGCCGCGCTAAGCGATAACTATCTCAAAGAGC
GGGCTGGCGATTTACGTGCGCTGGGTCAGCGATTGCTGTTTCATCTTGATGACGCTAATCAAGGGCCGAACGCCTGGCCG
GAACGTTTCATTCTGGTGGCAGATGAACTGTCAGCGACAACGCTTGCTGAGCTGCCCCAGGATCGCTTAGTCGGTGTTGT
CGTGCGCGATGGCGCTGCCAACTCCCATGCTGCGATCATGGTACGTGCGCTGGGGATACCTACCGTGATGGGCGCGGATA
TTCAGCCTTCGGTACTGCATCGTCGGACGCTGATCGTCGATGGTTATCGCGGTGAATTGCTGGTCGATCCGGAGCCGGTA
CTGCTGCAAGAATATCAGCGGCTAATTAGTGAAGAGATCGAGCTTAGCCGTCTGGCGGAAGATGACGTCAATTTACCCGC
CCAGTTAAAAAGCGGCGAACGCATTAAAGTCATGCTCAATGCCGGTTTAAGCCCGGAACATGAAGAAAAACTGGGCAGCC
GTATTGATGGCATAGGACTTTATCGCACTGAAATCCCATTCATGCTGCAAAGTGGTTTTCCGTCGGAAGAAGAACAGGTG
GCGCAGTATCAGGGGATGCTGCAAATGTTTAATGATAAACCCGTCACCTTGCGTACGCTGGATGTCGGAGCAGATAAGCA
GCTGCCTTACATGCCGATCAGCGAAGAGAATCCATGCCTGGGTTGGCGTGGGATTCGCATTACGCTCGATCAGCCGGAGA
TCTTCTTGATCCAGGTGCGGGCGATGCTGCGTGCTAATGCCGCTACGGGCAACCTGAATATTCTGTTGCCGATGGTCACA
AGCCTCGATGAAGTTGACGAAGCACGCCGCCTGATTGAACGTGCCGGACGTGAAGTCGAGGAGATGATCGGTTACGAAAT
TCCTAAACCACGTATCGGCATCATGCTGGAAGTGCCGTCAATGGTATTTATGCTGCCGCATCTGGCAAAGCGGGTCGATT
TCATCTCTGTTGGCACCAACGATCTGACTCAATACATCCTGGCCGTTGATCGCAACAATACCCGGGTGGCGAACATTTAT
GACAGTCTTCATCCTGCAATGTTACGAGCTCTGGCGATGATCGCCCGGGAAGCGGAAATACATGGAATCGATCTCCGTTT
GTGCGGTGAAATGGCGGGCGATCCCATGTGCGTGGCAATCCTCATTGGGCTTGGGTATCGCCATCTGTCTATGAACGGAC
GTTCTGTAGCGCGCGTAAAATACCTGCTGCGGCGCATTGATTTTGCTGAAGCAGAAAATCTTGCGCAGCGTAGTCTGGAA
GCGCAACTGGCGACCGAAGTTCGCCATCAGGTTGCAGCCTTTATGGAGCGTCGCGGCATGGGCGGGCTGATTCGCGGGGG
GTTATAG

Upstream 100 bases:

>100_bases
GAAAGAGTTCGCGAGTGTGGTGATGTCACTGCAGGAAAATACGCCAAAACCACAAAACGCATCTGCTTATCGACGTAAAA
GAGGTTAAGTCACGCCAATT

Downstream 100 bases:

>100_bases
CGCGGATCATATACATATCTTTTAACGGTATCCGGCAACCAGCCAGGTCCCCTTGTGCTATTATTCGCACCTTTGGAGCG
CCTGAAACCTGCGGCGCGCA

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAVILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWP
ERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPV
LLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVT
SLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLE
AQLATEVRHQVAAFMERRGMGGLIRGGL

Sequences:

>Translated_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAVILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWP
ERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPV
LLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVT
SLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLE
AQLATEVRHQVAAFMERRGMGGLIRGGL
>Mature_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAVILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWP
ERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPV
LLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVT
SLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLE
AQLATEVRHQVAAFMERRGMGGLIRGGL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=99.5989304812834, Blast_Score=1512, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=578, Percent_Identity=33.7370242214533, Blast_Score=296, Evalue=4e-81,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=33.2669322709163, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=32.1223709369025, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1787994, Length=406, Percent_Identity=25.8620689655172, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI226510935, Length=178, Percent_Identity=28.0898876404494, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83757; Mature: 83757

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHEEEEECCCCCEEEEEECCCCCC
PRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQYDESEESFLVTLATQMAVILSQSQLTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIM
HHHHHEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLNILLPMVTSLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPS
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECHH
MVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEI
HHHHHHHHHHHCCEEECCCHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
HGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLE
CCEEHEEHHHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
AQLATEVRHQVAAFMERRGMGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHEEEEECCCCCEEEEEECCCCCC
PRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQYDESEESFLVTLATQMAVILSQSQLTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIM
HHHHHEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLNILLPMVTSLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPS
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECHH
MVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEI
HHHHHHHHHHHCCEEECCCHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
HGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLE
CCEEHEEHHHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
AQLATEVRHQVAAFMERRGMGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]