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The map label for this gene is csdA [H]

Identifier: 218690934

GI number: 218690934

Start: 3200778

End: 3201983

Strand: Direct

Name: csdA [H]

Synonym: ECED1_3264

Alternate gene names: 218690934

Gene position: 3200778-3201983 (Clockwise)

Preceding gene: 218690932

Following gene: 218690935

Centisome position: 61.44

GC content: 55.97

Gene sequence:

>1206_bases
ATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCGCTACAGGATGCGGGCGTCTATCTCGACAGCGCCGC
GACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCGACAGTTTTATAGCCTGAGCGCCGGAAACGTCCATCGCAGCC
AGTTTGCCGAAGCCCAACGCCTGACCGCGCGTTACGAAGCTGCGCGGGAAAAAGTGGCACAATTACTGAATGCATCGGAT
GACAAAAATATCGTCTGGACGCGCGGCACCACTGAATCCATCAACATGGTGGCGCAATGCTATGCCCGTCCGCGTCTGCA
ACCGGGCGATGAAATTATTGTCAGTGTGGCAGAACACCACGCCAACCTCGTCCCCTGGCTGATGGTCGCCCAACAAACGG
GGGCCAAAGTGGTGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGT
AGTCGGATTCTGGCGTTGGGGCAGATGTCGAACGTCACTGGCGGTTGCCCGGATCTGGCGAGAGCGATTACTTTTGCTCA
TTCAGCCGGGATAGTGGTGATGGTTGATGGTGCTCAGGGGGCGGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTG
ATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGCAAACCAGAACTGCTGGAA
GCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGAAGGCTTCACGACTCAATCTGCGCCGTG
GAAACTGGAAGCAGGAACGCCAAATGTCGCTGGCGTCATAGGATTAAGCGCGGCGCTGGAATGGCTGACAGATTACGATA
TCAACCAGGCCGAAAGCTGGAGCCGTAGCTTAGCAACGCTTGCAGAAGAAGCGCTGGCGAAACGTCCAGGCTTTCGTTCA
TTCCGCTGCCAGGATTCCAGCCTGCTGGCCTTTGATTTTGCGGGTGTTCATCACAGCGATATGGTGACACTGCTGGCGGA
GTACGGTATTGCCCTGCGCGCCGGGCAGCATTGCGCTCAGCCGCTACTGGCAGAATTAGGCGTAACCGGCACACTGCGCG
CCTCATTTGCGCCATATAATACAAAGAGTGATGTGGATGCGCTGGTGAATGCCGTTGACCGCGCGCTGGAATTATTGGTG
GATTAA

Upstream 100 bases:

>100_bases
GCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGT
GCATCAAGCCGAGGAGTACC

Downstream 100 bases:

>100_bases
TGACAAACCCGCAATTCGCCGGACATCCGTTCGGCACAACCGTAACCGCAGAAACGTTACGCAATACCTTCGCACCGTTG
ACGCAATGGGAAGATAAATA

Product: cysteine sulfinate desulfinase

Products: @ALAN01txt*L-Alanine! [C]

Alternate protein names: CSD [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASD
DKNIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D

Sequences:

>Translated_401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASD
DKNIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D
>Mature_401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASD
DKNIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway f

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=26.0204081632653, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI156713448, Length=433, Percent_Identity=25.1732101616628, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=98.0049875311721, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=404, Percent_Identity=43.3168316831683, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI48994898, Length=215, Percent_Identity=30.6976744186047, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=213, Percent_Identity=30.5164319248826, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI193211090, Length=362, Percent_Identity=25.414364640884, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=27.6807980049875, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=27.7777777777778, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR022471
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 4.4.1.- [C]

Molecular weight: Translated: 43316; Mature: 43316

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYSLSAGNVHRSQFAEAQR
CCCCCCHHHHHHCCCHHCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLA
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCHHHEEEHHCCCCCCCCCHHHH
RAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKPELLE
HHHHHHCCCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCCCEEEECCHHHHH
AMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLTDYDINQAESW
HHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYSLSAGNVHRSQFAEAQR
CCCCCCHHHHHHCCCHHCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLA
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCHHHEEEHHCCCCCCCCCHHHH
RAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKPELLE
HHHHHHCCCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCCCEEEECCHHHHH
AMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLTDYDINQAESW
HHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cystine; L-selenocystine [C]

Specific reaction: Catalyzes the removal of elemental sulfur and selenium atoms from l-cysteine, l-cystine, l-selenocysteine, and l- selenocystine to produce @ALAN01.txt*l-Alanine! [C]

General reaction: Remove sulfur and selenium atoms [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278392; 9278503; 10829016; 10739946 [H]