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The map label for this gene is yfiN [H]

Identifier: 218690722

GI number: 218690722

Start: 3002635

End: 3003861

Strand: Direct

Name: yfiN [H]

Synonym: ECED1_3043

Alternate gene names: 218690722

Gene position: 3002635-3003861 (Clockwise)

Preceding gene: 218690721

Following gene: 218690723

Centisome position: 57.64

GC content: 48.17

Gene sequence:

>1227_bases
ATGATGGATAACGATAATTCTCTTCATAAGCGCCCCACGTTTAAAAGAGCATTACGCAACATCAGCATGACCAGCATATT
TATCACTATGACGCTGATCTGGCTGCTGCTTTCCGTGACGTCAGTGCTAACCCTGAAACAATACGCGCAAAAAAACCTGG
CACTGACAGCAGCAACAATGACTTACAGTCTGGAAGCAGCTGTCGTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTG
GCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTGCAGAAGTGCGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTA
CACCCATAAGGAACCAGGCGATACTTTCAGCAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTC
GCCACAATGGTGAAACTATTGGCGAAGTACGCTTAACTGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTC
GCTGTCCTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTGGA
AGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCTAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTA
TCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTACAGGCC
AAAAATGCGCAGCTTCTACGTACCGCGCTACATGACCCATTAACCGGGCTGGCTAACCGCGCAGCGTTTCGTAGCGGCAT
CAACACGTTGATGAACAATTCCGATGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATATATCA
ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGA
CATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGAT
ATGCTCAGCACTGACACAAATTTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACTACCATGACATTAAGCATTG
GTTACGCGATGACTATTGAGCACGCCTCTGCGGAAAATTTACAAGAGCTTGCCGATCACAATATGTATCAGGCCAAACAC
CAGCGTGCCGAAAAGCTGGTGAGATAA

Upstream 100 bases:

>100_bases
ATCCACAATAATGACGTCAGATTTGCCGTAAACCTGGATGCCTTATCGCGTAGCGGTGTAAAGGTCAACCCGGATGTCTT
AATGCTCGCACGGAAGAAAA

Downstream 100 bases:

>100_bases
CAAGGATATATCGATATGATAAAGCACCTGCTAGCACCCCTGATTTTCACCTCGCTAATACTTACTGGCTGCCAGTCCCC
TCAGGGAAAGTTTACTCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR

Sequences:

>Translated_408_residues
MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR
>Mature_408_residues
MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=408, Percent_Identity=98.7745098039216, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=153, Percent_Identity=32.6797385620915, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=35.5263157894737, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.0512820512821, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=30.0613496932515, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=32.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786584, Length=150, Percent_Identity=30, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081977, Length=101, Percent_Identity=41.5841584158416, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45964; Mature: 45964

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTHKEPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKHQRAEKLVR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTHKEPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKHQRAEKLVR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]