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The map label for this gene is fadI [H]

Identifier: 218690501

GI number: 218690501

Start: 2745656

End: 2746966

Strand: Reverse

Name: fadI [H]

Synonym: ECED1_2805

Alternate gene names: 218690501

Gene position: 2746966-2745656 (Counterclockwise)

Preceding gene: 218690502

Following gene: 218690500

Centisome position: 52.73

GC content: 55.76

Gene sequence:

>1311_bases
ATGGGTCAGGTTTTACCGCTGGTTACCCGCCAGGGCGATCGTATCGCCATTGTTAGCGGTTTACGTACGCCTTTTGCCCG
ACAGGCGACGGCTTTTCATGGCATTCCCGCAGTTGATTTAGGGAAGATGGTGGTAGGCGAACTGCTGGCACGCACCGAGA
TCCCCGCTGAAGTGATTGAACAACTGGTCTTCGGTCAGGTTGTGCAAATGCCGGAAGCGCCCAACATTGCCCGTGAAATT
GTTCTTGGCACTGGAATGAATGTGCATACCGATGCTTACAGCGTTAGCCGCGCTTGCGCTACCAGTTTCCAGGCGGTTGC
AAACGTCGCAGAAAGCCTGATGGCGGGAACTATTCGAGCGGGGATTGCCGGTGGGGCAGATTCCTCTTCCGTATTGCCAA
TTGGCGTCAGTAAAAAACTGGCGCGCGTACTGGTTGATGTCAACAAAGCCCGCACAATGAGCCAGCGCCTGAAACTTTTT
TCTCGCTTACGTTTGCGTGACTTAATGCCGGTGCCTCCGGCAGTGGCGGAATACTCCACTGGATTACGGATGGGGGACAC
CGCAGAGCAAATGGCGAAAACCTACGGCATCACCCGAGAACAGCAAGATGCACTGGCGCACCGTTCGCATCAGCGTGCCG
CTCAGGCGTGGTCAGAAGGTAAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCCCCTTATAAACAACCGCTTGTCGAA
GACAACAATATTCGCGGTAATTCCTCTCTTGCTGATTACGCAAAGCTGCGTCCGGCGTTTGATCGTAAACACGGGACGGT
AACGGCGGCGAACAGTACGCCGCTGACCGATGGCGCGGCAGCGGTGATCCTGATGACTGAATCCCGGGCGAAAGAATTAG
GGCTGGTACCGCTGGGGTATCTGCGCAGCTACGCATTTACTGCGATAGATGTCTGGCAGGACATGTTGCTCGGTCCAGCC
TGGTCAACACCGCTGGCGCTGGAACGTGCCGGATTGACGATGGGCGATCTGACATTGATCGATATGCACGAAGCCTTTGC
CGCCCAGACGCTGGCCAATATTCAGTTGCTGGGTAGCGAACGTTTTGCTCGCGACGTACTGGGGCGTGCACATGCCACTG
GCGAAGTGGACGAGAGCAAATTTAACGTGCTTGGCGGTTCGATTGCTTATGGGCATCCCTTCGCAGCGACCGGCGCGCGG
ATGATTACCCAGACATTGTATGAACTTCGCCGTCGTGGCGGTGGATTTGGTTTAGTGACCGCCTGTGCTGCGGGTGGGCT
TGGCGCAGCAATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
CTCCCACTTTCCCTTTTCTCCACTTGGCTAAATGTAAAAAAATGGTTAAGACTATGATTAGGTCAGACCACTTTATTTAT
TTTTTTTACAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TGGAAATGGCATCAGCGTTTACTCTTAATGTTCGTCTGGACAACATTGCTATCATCACCATCGACGTACCGGGTGAGAAA
ATGAATACCCTGAAGGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGAR
MITQTLYELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGAR
MITQTLYELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_435_residues
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIV
LGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFS
RLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVED
NNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAW
STPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGARM
ITQTLYELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=422, Percent_Identity=42.1800947867299, Blast_Score=341, Evalue=8e-94,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=29.5508274231678, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=31.8604651162791, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=30.188679245283, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=29.6728971962617, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=36.7924528301887, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=98.394495412844, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.9383886255924, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.4609929078014, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI48994986, Length=437, Percent_Identity=35.0114416475973, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=32.8638497652582, Blast_Score=182, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=38.5342789598109, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.2206572769953, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.0952380952381, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=29.2452830188679, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=420, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325229, Length=438, Percent_Identity=30.8219178082192, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=440, Percent_Identity=28.4090909090909, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=41.1627906976744, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.6208530805687, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.4647887323944, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17648125, Length=430, Percent_Identity=29.3023255813954, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46583; Mature: 46452

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIE
CCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGARMITQTLYELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIE
CCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGARMITQTLYELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA