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The map label for this gene is gne [H]

Identifier: 218690100

GI number: 218690100

Start: 2322029

End: 2323024

Strand: Reverse

Name: gne [H]

Synonym: ECED1_2388

Alternate gene names: 218690100

Gene position: 2323024-2322029 (Counterclockwise)

Preceding gene: 218690101

Following gene: 218690099

Centisome position: 44.59

GC content: 45.18

Gene sequence:

>996_bases
ATGAACGATAACGTTTTGCTCATAGGAGCTTCCGGATTCGTAGGAACCCGACTACTTGAAACGGCAATTGCTGACTTTAA
TATCAAGAACCTGGACAAACAGCAGAGCCACTTTTATCCAGAAATCACACAGATTGGCGATGTTCGTGATCAACAGGCAC
TCGACCAGGCGTTAGCCGGTTTTGACACTGTTGTGCTACTGGCAGCGGAACACCGCGATGACGTCAGCCCTACTTCTCTC
TATTATGATGTCAACGTTCAGGGTACCCGCAATGTGCTGGCGGCCATGGAAAAAAATGGCGTTAAAAATATCATCTTTAC
CAGTTCCGTTGCTGTTTATGGTTTGAACAAACACAACCCTGACGAAAACCATCCGCACGATCCTTTCAACCACTACGGCA
AAAGTAAGTGGCAGGCAGAGGAAGTGCTGCGTGAATGGTATAACAAAGCACCAACAGAACGTTCATTAACCATCATCCGT
CCTACCGTCATCTTCGGTGAACGAAACCGCGGTAACGTCTATAACTTGCTGAAACAGATCGCTGGCGGCAAGTTTATGAT
GGTGGGCGCAGGGACTAACTATAAGTCCATGGCTTATGTTGGAAACATTGTTGAGTTTATCAAGTACAAACTGAAGAATG
TTGCCGCAGGTTATGAGGTTTATAACTACGTTGATAAGCCAGACCTGAACATGAACCAGTTGGTTGCTGAAGTTGAACAA
AGCCTGAACAAAAAGATCCCTTCTATGCACTTGCCTTACCCACTAGGAATGCTGGGTGGATATTGTTTTGATATCCTGAG
CAAAATTACGGGCAAAAAATACACTGTCAGCTCAGTGCGCGTGAAAAAATTCTGCGCAACAACACAGTTTGACGCAACGA
AAGTGCATTCTTCAGGTTTTGTGGCACCGTATACGCTGTCGCAAGGTCTGGATCGAACTCTGCAGTATGAATTCGTTCAT
GCCAAAAAAGACGACATAACGTTTGTTTCTGAGTAA

Upstream 100 bases:

>100_bases
CACTAACTGTTAAAGCTGGGGCTGGGCAGCATTGACTACTCAGGGAAGGTACTCATCTTTATCTTGTGTTTCTTACGTAA
CAACCAGACTAAAGTGATTT

Downstream 100 bases:

>100_bases
TGCAGACGAGTCAGAGAAAAAAGGACCAGGTTGGACTACGATTATTCGGAAAGAGGTACTGATTAAATTATCAGTATGCG
GAATTCCGAGTAATCCGAGT

Product: UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase)

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYTVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Sequences:

>Translated_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYTVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE
>Mature_331_residues
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSL
YYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYTVSSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVH
AKKDDITFVSE

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI8393516, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI56237023, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI56118217, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI189083684, Length=294, Percent_Identity=28.2312925170068, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786974, Length=299, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081792, Length=307, Percent_Identity=24.7557003257329, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321437, Length=345, Percent_Identity=26.3768115942029, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 37173; Mature: 37173

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYTVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQALAG
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHC
FDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYGLNKHNP
CCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCEEEEECCCCCC
DENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGNVYNLLKQI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLNMNQLVAEVEQ
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYTVSSVRVKKFCATTQFDATKVHSSGF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHEECCCCC
VAPYTLSQGLDRTLQYEFVHAKKDDITFVSE
CCCHHHHHCCCHHEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]