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The map label for this gene is php [H]

Identifier: 218690059

GI number: 218690059

Start: 2279484

End: 2280518

Strand: Direct

Name: php [H]

Synonym: ECED1_2342

Alternate gene names: 218690059

Gene position: 2279484-2280518 (Clockwise)

Preceding gene: 218690058

Following gene: 218690063

Centisome position: 43.76

GC content: 47.34

Gene sequence:

>1035_bases
ATGAAAGATTATCTACAAACTGTGACGGGCCCCGTCGCCCGTGAAGATATGGGGCTAACACTTCCTCATGAACATCTGTT
CAACGATCTTTCCTCTGTTGTGGATGCTCCTTGCTATCCTTTTTCACAACAGCTTGTTGATAAAAAAGTCACGGCAGAAA
TCCAGTGGGCATTAAAACACGATCCTTATTGCTGCGCTGATAATATGGACCGCAAACCTATTGAAGATGTGATCTTCGAA
ATTAATAACTTTATCTCGCTCGGTGGTCGCACCATTGTTGATGCCACAGGGTCTGAGTCTATCGGACGAGATGCGCAGGC
ATTACGAGAAGTCGCATTAAAAACAGGTCTGAATATTGTTGCTTCCTCCGGTCCCTACCTGGAAAAATTTGAAAGCCAGA
GAATTCATAAAACGGTTGATGAACTAGCGACAACGATCGATAAAGAATTGAATCAAGGGATTGGCGATACGGATATTCGT
GCCGGAATGATCGGTGAAATAGGTGTCTCACCGACATTTACCGAAGCCGAGCATAACAGCTTGCGGGCTGCCTCGCTGGC
ACAGATTAACAATCCTCATGTGGCGATGAATATTCACATGCCGGGCTGGCTTCGTCGCGGTGATGAAGTACTCGACATTG
TGTTAGATGAAATGGGCGTCTCGCCAAATAAAGTCTCTCTCGCACACTCGGATCCGTCAGGAAAAGACGTGGCGTATCAG
CGGAAAATGCTTGATAAAGGTGTCTGGCTGGAATTCGACATGATTGGCCTAGACATTACCTTCCCGAAAGAGGGAATAGC
GCCAGGGGTGCAGGAGACTGCCGATGCCGTCGCTCATCTCATTGAGTTGGGATACGCCGATCAGCTTGTTCTCAGCCACG
ATGTCTTCCTTAAACAAATGTGGGCTAAAAATGGCGGTAATGGCTGGGGATTTGTTCCAGATGTTTTTCTGGCCTATCTG
GCGGAGCGCGGCGTCGATAAAACGATCCTCAAAAAACTCTGTATCGATAATCCCGGACGATTATTGACCGCATAA

Upstream 100 bases:

>100_bases
TCACGATCATCGGAACACTGTTTGCACTGGTCGGTGCAATTGTTACCACTATTCCGGAATACAGAGCCTCGAAAACAATG
AAGAAAATGGAGGTATAATC

Downstream 100 bases:

>100_bases
AACCATTATCCCCCGGCACATTGCGCCGGGGGATGTCAATTAATACCCCGCCGTTAAATCATCCACCAAGCGCGAGTCGG
ATGCGCCGTACAACTCACCA

Product: putative phosphotriesterase-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQLVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFE
INNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLDEMGVSPNKVSLAHSDPSGKDVAYQ
RKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYL
AERGVDKTILKKLCIDNPGRLLTA

Sequences:

>Translated_344_residues
MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQLVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFE
INNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLDEMGVSPNKVSLAHSDPSGKDVAYQ
RKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYL
AERGVDKTILKKLCIDNPGRLLTA
>Mature_344_residues
MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQLVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFE
INNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLDEMGVSPNKVSLAHSDPSGKDVAYQ
RKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYL
AERGVDKTILKKLCIDNPGRLLTA

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=348, Percent_Identity=28.735632183908, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI47933341, Length=348, Percent_Identity=28.735632183908, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1789780, Length=327, Percent_Identity=27.5229357798165, Blast_Score=103, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645325, Length=348, Percent_Identity=22.9885057471264, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 37825; Mature: 37825

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01322 PHOSPHOTRIESTERASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQLVDKKVTAEIQWALKH
CCHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECC
DPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIV
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEE
ASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
LRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLDEMGVSPNKVSLAHSDPSGKDVAYQ
HHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
RKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQM
HHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA
HHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQLVDKKVTAEIQWALKH
CCHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECC
DPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQALREVALKTGLNIV
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEE
ASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIRAGMIGEIGVSPTFTEAEHNS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
LRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLDEMGVSPNKVSLAHSDPSGKDVAYQ
HHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
RKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHLIELGYADQLVLSHDVFLKQM
HHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLCIDNPGRLLTA
HHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]