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The map label for this gene is shiA

Identifier: 218690010

GI number: 218690010

Start: 2231357

End: 2232673

Strand: Direct

Name: shiA

Synonym: ECED1_2287

Alternate gene names: 218690010

Gene position: 2231357-2232673 (Clockwise)

Preceding gene: 218690009

Following gene: 218690011

Centisome position: 42.83

GC content: 50.42

Gene sequence:

>1317_bases
ATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAG
CTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTT
TCCCGCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGC
GGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGC
GACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCA
TTCAGGGATTTGCAGTCGGCGGCGAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCC
TTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGATCAGTATGAT
GACGACTGACGAACAGTTTTTAAGCTGGGGCTGGCGCATTCCTTTCCTGTTTAGCATCGTACTGGTACTGGGAGCATTGT
GGGTGCGCAATGGCATGGAGGAGTCCGCGGAATTTGAACAACAGCAACATTATCAAGCTGCCGCGAAAAAACGCATCCCG
GTTATCGAAGCGCTGTTACGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTA
CATCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGC
TGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATC
ACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTT
CTTCTCCATAATGCTGGCAAACATTGCGCATGACATGGTGGTGTGTGTGCAACAACCGATGTTTACCGAAATGTTTGGTG
CCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAGTGTGGTTGGCGGTGGATTTACACCTTTTATTGCC
GCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAAT
GACCGCTTTGTTGATGAAAGACAGTCAACGCGCTTGA

Upstream 100 bases:

>100_bases
CTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAG
ACAGTACAAACAGGATCATT

Downstream 100 bases:

>100_bases
TAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAAT
TGTATGGAACAGGAGACACA

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIP
VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYI
TGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIA
AALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA

Sequences:

>Translated_438_residues
MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIP
VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYI
TGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIA
AALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA
>Mature_438_residues
MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIP
VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYI
TGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIA
AALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Homologues:

Organism=Escherichia coli, GI1788292, Length=438, Percent_Identity=100, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1789941, Length=442, Percent_Identity=35.5203619909502, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790550, Length=443, Percent_Identity=31.3769751693002, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788942, Length=400, Percent_Identity=31.25, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082404, Length=399, Percent_Identity=24.3107769423559, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87082231, Length=227, Percent_Identity=25.1101321585903, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SHIA_ECOLI (P76350)

Other databases:

- EMBL:   U88529
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G64962
- RefSeq:   AP_002585.1
- RefSeq:   NP_416488.1
- ProteinModelPortal:   P76350
- STRING:   P76350
- PRIDE:   P76350
- EnsemblBacteria:   EBESCT00000000787
- EnsemblBacteria:   EBESCT00000015319
- GeneID:   946495
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1962
- KEGG:   eco:b1981
- EchoBASE:   EB4148
- EcoGene:   EG20205
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008829
- HOGENOM:   HBG757988
- OMA:   SVQQPMF
- ProtClustDB:   PRK09952
- BioCyc:   EcoCyc:SHIA-MONOMER
- Genevestigator:   P76350
- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829
- TIGRFAMs:   TIGR00883

Pfam domain/function: PF00083 Sugar_tr; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 47818; Mature: 47818

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21694940)-; HASH(0x219ab250)-; HASH(0x21cc711c)-; HASH(0x20ae6e90)-; HASH(0x204b5008)-; HASH(0x213db144)-; HASH(0x21d7aafc)-; HASH(0x21fdb57c)-; HASH(0x21cf9194)-; HASH(0x21ce8bac)-; HASH(0x21cd3878)-; HASH(0x220a031c)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503