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The map label for this gene is traC [H]

Identifier: 218690003

GI number: 218690003

Start: 2213451

End: 2214497

Strand: Reverse

Name: traC [H]

Synonym: ECED1_2276

Alternate gene names: 218690003

Gene position: 2214497-2213451 (Counterclockwise)

Preceding gene: 218690004

Following gene: 218689974

Centisome position: 42.51

GC content: 51.1

Gene sequence:

>1047_bases
TTGCACGCATACTGCGCCCTTGCAGGACAGAACGATGCCGGGAAAGCGAACCGTCAGGCGATACGAAGACGAAAATCACA
CACTGACGGAGAAAAAACCATGACGATTTCCCTTTATACCTCCCGGACAAGCGCCCCTAACACCGCAGCGGCGACCAGCG
TATCCTCGCTGGAGCAGTCAGGCTCCTCAAAAACGAAATTTTCTAAAACCAGAACGGATATTTATCAGACCGTTACCGAC
AGCATCATTGCAGCGCTGGAAGCCGGTGTAAAACCGTGGTCCTGCCCGTGGCTGCGTGTATCCGGCATGTCTGGACTGCC
ATCCAATTACGCCACTGGCACGGCTTACAGCGGAATGAATATCATGTTGCTGTGGTGCAGCGCATCTAAACAGGGTTTCA
GCGATTCCCGCTGGATGACTTACAAACAGGCACAGGCAGCAGGTGGACAGGTTCGCAAGGGTGAACATGGCACAACAGCC
ATTTTCTATACGACCCTGGAAAAGGAAAACGACGCCGGAGAGGTTGAACAAATTCCGATGCTGAAAACTTTCACCGTGTT
TAACGTTCAGCAAATCGACGGTTTGCCCCTGACAACTGAAACAGTCAGCCCGGAAGCTACATTTAACCCGTTGCCGCAGG
CTGAGAACCTGTTACGGAAGAGTGGCGCAAACATCATCGAGAAAGGACAAAACGCCTTTTTCAGCCCATCAACTGATGAA
GTCTGGTTACCGGAGCGCCATTTGTTTTCGGATGCGGCTAACTTCTACGCTACCGGTCTGCATGAGCTGGTTCACTGGAG
CGGGGGTAAAAAACGCCTGAACCGTGAAATGAAAGGGAAGTTTGGCAGTGCGGATTATGCAGAGGAAGAACTTGTCGCGG
AGCTGGGAAGTGCCTTTCTGATGGCTGATCTTGGCATTGAGGGAGAGGTACAACATGAAAGCTATATTGCTTCCTGGCTG
AAAGCGCTGAAAAACGACAAGCGTTATATTTTCAAAGCGGTCAGCGCCGCCTCTAAAGCGCATTCTTATCTGATGGATAA
ACTCTGA

Upstream 100 bases:

>100_bases
GCTATGCAGGGGCAGGTTTTCTCTCCCGTGCGCTTTTCCGTTCTTCATATCTGCTTCCGGTTAATCGTTTCCCAACACAA
CCCGTCCCGCAAGGGTAAAA

Downstream 100 bases:

>100_bases
GGACGGAGCCAGACAACCGCAAGGGAATGCGGTTACATGGTTTTTAACCGGCAACGCTGCAGGGCGCAGACTGAGGCGGA
ACGCAGCATAGCGAGTACCG

Product: putative Antirestriction protein ardC

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MHAYCALAGQNDAGKANRQAIRRRKSHTDGEKTMTISLYTSRTSAPNTAAATSVSSLEQSGSSKTKFSKTRTDIYQTVTD
SIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMNIMLLWCSASKQGFSDSRWMTYKQAQAAGGQVRKGEHGTTA
IFYTTLEKENDAGEVEQIPMLKTFTVFNVQQIDGLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDE
VWLPERHLFSDAANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMADLGIEGEVQHESYIASWL
KALKNDKRYIFKAVSAASKAHSYLMDKL

Sequences:

>Translated_348_residues
MHAYCALAGQNDAGKANRQAIRRRKSHTDGEKTMTISLYTSRTSAPNTAAATSVSSLEQSGSSKTKFSKTRTDIYQTVTD
SIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMNIMLLWCSASKQGFSDSRWMTYKQAQAAGGQVRKGEHGTTA
IFYTTLEKENDAGEVEQIPMLKTFTVFNVQQIDGLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDE
VWLPERHLFSDAANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMADLGIEGEVQHESYIASWL
KALKNDKRYIFKAVSAASKAHSYLMDKL
>Mature_348_residues
MHAYCALAGQNDAGKANRQAIRRRKSHTDGEKTMTISLYTSRTSAPNTAAATSVSSLEQSGSSKTKFSKTRTDIYQTVTD
SIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMNIMLLWCSASKQGFSDSRWMTYKQAQAAGGQVRKGEHGTTA
IFYTTLEKENDAGEVEQIPMLKTFTVFNVQQIDGLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDE
VWLPERHLFSDAANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMADLGIEGEVQHESYIASWL
KALKNDKRYIFKAVSAASKAHSYLMDKL

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 38186; Mature: 38186

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYCALAGQNDAGKANRQAIRRRKSHTDGEKTMTISLYTSRTSAPNTAAATSVSSLEQS
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHC
GSSKTKFSKTRTDIYQTVTDSIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEECCCE
IMLLWCSASKQGFSDSRWMTYKQAQAAGGQVRKGEHGTTAIFYTTLEKENDAGEVEQIPM
EEEEEECCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCC
LKTFTVFNVQQIDGLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDE
EEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCC
VWLPERHLFSDAANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFL
EECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHE
MADLGIEGEVQHESYIASWLKALKNDKRYIFKAVSAASKAHSYLMDKL
EECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHAYCALAGQNDAGKANRQAIRRRKSHTDGEKTMTISLYTSRTSAPNTAAATSVSSLEQS
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHC
GSSKTKFSKTRTDIYQTVTDSIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEECCCE
IMLLWCSASKQGFSDSRWMTYKQAQAAGGQVRKGEHGTTAIFYTTLEKENDAGEVEQIPM
EEEEEECCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCC
LKTFTVFNVQQIDGLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDE
EEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCC
VWLPERHLFSDAANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFL
EECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHE
MADLGIEGEVQHESYIASWLKALKNDKRYIFKAVSAASKAHSYLMDKL
EECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]