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The map label for this gene is virB4 [H]

Identifier: 218689990

GI number: 218689990

Start: 2199956

End: 2202694

Strand: Direct

Name: virB4 [H]

Synonym: ECED1_2263

Alternate gene names: 218689990

Gene position: 2199956-2202694 (Clockwise)

Preceding gene: 218689989

Following gene: 218689991

Centisome position: 42.23

GC content: 53.19

Gene sequence:

>2739_bases
ATGGCAAAGCTGCTCAAGGCAATGAAACGCCCGGCGGCGCTGTGGGGGGTGCCAATGGTGCCCCTGCTGGCTGTCACGGG
TGTAACCATCATCGTTGCTATCTGGACTTCCGTCGCTCTCCTGTTCTTACTTCCTGTGCAATTTCTGGTGATGAAGTCCC
TTACCAGAAATGAACCGATGCGGTTCAATCTGATTGCCGTATGGCTGAGAGCGAAGGGAAAGCCGGTAGCAAACCGTTTA
TTTGGCGCAACAACATTTATGCCCGTTGAGCATGATGATGTTGATATCAAGGAGTTTCTCGACGCCATGAAACTAAATCA
GTGTGCCACCATCAAGAAGTACATCCCGTACTCATCGCACATTCATCAGCATGTGGTTCGTTCCCCTAAGAGCGATCTGT
ACTGCACCTGGGAATTGATGGGAACGCCGTTCGACTGCGAGTCTGACGAGTCGCTCCAGTTCGGGACGAACCAGTTACAC
GGGCTAATCCGCTCATTCGAAGGTATGCCAGTTACTTTCTATATCCATAACGACCGCAATACGTTTACCGACAATCTTCA
TAAAGATTCCGGCAACCCGTATGCCGATGAAGTTAGCCGTCTTTACTACGCTTCTGTCGGTCTGTTCCGGCGCAACCGCC
TGTTCCTGACGGTCTGCTATCGCCCGTTTGTCAGCCTTGAAAAAGCAGAACGCAAACGGATGAAAGACAGCCAGAAACTA
AAGGAGCTGGACGGTGCTTTGCTGGAAATGCTGGAAATAAAAAGCACGCTCGATACCGCGCTGTCGCGCTATGGTGCGCG
ACCTCTCGGTACGTTTACCGAAGGAAACGCGGTGTTTTCGTCGCAACTGGCTTTCTATGAATATCTGCTAACGCACCAGT
GGCGAAAAGTGCGCGTCACCCGTACCCCGGCCTATGACGTGATGGGGGCGGCGGCGTTGTTCTTTTCGGCTGAATCCGGG
CAGATAAACCACGCCAACGGTACGCAATATTTCCGGGGGCTGGAGGTTAAAGAGTTCGCCGAAGAGACCGCCACGGGGAT
GATGGACTCCCTTCTTTACGCCCCCTGCGATTACGTGATCACGCAGTCCTATACATGCATGTCACGCGAGGAAGCGAAGA
AAGCCATTAAGCGTACCCGCCGCCTTTTGATGAGTGCCGACGACGATGCTGTTTCCCAGCGGCTGGATCTGGATGTGGCG
CTTGACCTGCTGACATCGGGGAAAATCGCTTACGGCAAGCACCATTTTTCCATCATGGTGTATTCCCCGTCGCTGGAAAG
CCTGGTTGCTGATACGAATGAAATCAGTAACGCGCTGAACAACATAGGGATAACGCCTGTTCCGGCAGAAATATCGCTTT
CAGCGGCGTATATGGCGCAACTGCCAGGCAACTACAACCTGAGGCCACGTAAAGGGGAGCTGAGTAGCCAGAACTTTGTG
GAGCTGGCCGCGCTCCATAATTTCTATCCGGGCAAGCGCGATAAAGCCCCCTGGGGGGATGCGATGGCTTTACTGCGCAC
CCCGTCAGGCGATGGCTACTATATCAACCTGCATAACACACTGGCAGATAAGGACGAATTCAACGAGAAGAACCCGGCCA
GTACCTGCATCCTCGGTACGAACGGTTCGGGTAAAACCATGTTGATGACGTTCTTTGAGATCATGCAGCAGAAATACGGG
CGCGAGGATAGCTTCTCCCCGGATGCCAAAACCAAACGGCTGACAACCGTTTACCTCGATAAAGACCGGGGCGCAGAGAT
GAACATCCGGGCGCTGGGTGGCCGTTACTATCGGGTTATCAGTGGTGAGTCTACAGGGTGGAACCCGTTCTCGTTGCCAG
CAACCAAACGCAATATCAATTTCATCAAGCAACTGATGAAAATCCTTTGTACCCGTAACGGCTCGACGATCTCCCCACGC
GATGAACGCCGTCTGAGTGATGCGGTCAATGCTGTGATGAATGACGAGCCGCAATATCGCGTCTACGGCATTACCCGAAT
GCTGGAAAATTTGCCTGAGCCGGCTACGAAAGAAGCCCAGGAAAACGGCCTTAGCATTCGGTTGTCACAGTGGGCGCAGG
GCGGTGAGTTCGGCTGGGTGTTTGATAACGAGTCTGACACGTTCGATATCAGCAACTGCGATAACTTCGGCATCGACGGA
ACGGAGTTCCTGGACGATGCAAGCGTATGTGCGCCAATCTCGTTTTATCTGCTGTACCGCATTACCAGCCTGCTTGACGG
GCGACGCCTGGTCATTTTCATGGATGAATTCTGGAAGTGGTTACGCGACCCGGTGTTTAAGGACTTTGCTTATAACAAGC
TGAAAACCATCCGTAAGCTGAACGGGATGCTGGTCGTCGGTACTCAATCCCCGGCAGAGATCATCAAAGATGATATCGCC
CCGGCAGTGATTGAGCAGTGCGGGACACAGATTCTGGCGGCGAACCCCAACGCCGACCGCGCACACTATGTTGACGGTAT
GAAGTTTGAACCGGAAGTGTTCGACGTGGTGAAAGGGCTTGACCCGCAAGCCCGCCAGTATGTTGTGGTGAAAAATCAGT
TCAAGCGCGGCGATACCAAACGTTTTGCCGCTCGTGTCACGCTCGACCTGTCGGGTATCGGCAGATACACCAAAGTCATG
AGCGGCGATGCCCCCAACCTCGAAATATTCGAATCGATTTACCGGGAAGGAATGCAGCCTCATGAATGGCTCGATACCTA
CCTGGCTAAAGCGCTCTGA

Upstream 100 bases:

>100_bases
CGAGAGCCTGTCGCAGTGCGGCTACATCATCATTGGCGGCATTTTAATTGGCGGAGGCAGTGAAATCGGCGCTCTGCTGA
TGAGCTAAGAGGCTGAACAT

Downstream 100 bases:

>100_bases
CCCAAAGGAGATATACGATGAAAACTCGCTTTCGTACTCTTATTCTGGCTTGCGTCATTGCAAGCCCACTGGCTCATGCT
GGTATTCCCGTCGCTGTTGA

Product: putative type IV secretory pathway VirB4 component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 912; Mature: 911

Protein sequence:

>912_residues
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRL
FGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLH
GLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEKAERKRMKDSQKL
KELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESG
QINHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVA
LDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFV
ELAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPR
DERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDG
TEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIA
PAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVM
SGDAPNLEIFESIYREGMQPHEWLDTYLAKAL

Sequences:

>Translated_912_residues
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRL
FGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLH
GLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEKAERKRMKDSQKL
KELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESG
QINHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVA
LDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFV
ELAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPR
DERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDG
TEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIA
PAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVM
SGDAPNLEIFESIYREGMQPHEWLDTYLAKAL
>Mature_911_residues
AKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLF
GATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLHG
LIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEKAERKRMKDSQKLK
ELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQ
INHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVAL
DLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVE
LAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGR
EDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPRD
ERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDGT
EFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIAP
AVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMS
GDAPNLEIFESIYREGMQPHEWLDTYLAKAL

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 103069; Mature: 102938

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPM
CHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSH
EEEEEEEEEECCCCHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
IHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRN
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
TFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEKAERKRMKDSQKL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
KELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVRVT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
RTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVI
ECCCHHHHHHHHHEEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHH
TQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
HCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCEEECCEEEEEEE
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFV
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ELAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGT
HHHHHHHCCCCCCCCCCCCHHEEEEECCCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEC
NGSGKTMLMTFFEIMQQKYGREDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVI
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEE
SGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPRDERRLSDAVNAVMNDEPQYR
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDG
EHHHHHHHHHCCCCCHHHHHHCCCEEEEHHCCCCCCEEEEECCCCCEEECCCCCCCCCCC
TEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKL
HHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHH
NGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
CCEEEEECCCHHHHHHHHCCHHHHHHCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHCC
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQP
CHHHHEEEEEEHHCCCCCCHHEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCH
HEWLDTYLAKAL
HHHHHHHHHHCC
>Mature Secondary Structure 
AKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPM
HHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSH
EEEEEEEEEECCCCHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
IHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRN
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
TFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEKAERKRMKDSQKL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
KELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVRVT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
RTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVI
ECCCHHHHHHHHHEEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHH
TQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
HCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCEEECCEEEEEEE
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFV
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ELAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGT
HHHHHHHCCCCCCCCCCCCHHEEEEECCCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEC
NGSGKTMLMTFFEIMQQKYGREDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVI
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEE
SGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPRDERRLSDAVNAVMNDEPQYR
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDG
EHHHHHHHHHCCCCCHHHHHHCCCEEEEHHCCCCCCEEEEECCCCCEEECCCCCCCCCCC
TEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKL
HHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHH
NGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
CCEEEEECCCHHHHHHHHCCHHHHHHCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHCC
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQP
CHHHHEEEEEEHHCCCCCCHHEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCH
HEWLDTYLAKAL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]