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The map label for this gene is irp [H]

Identifier: 218689980

GI number: 218689980

Start: 2190874

End: 2192451

Strand: Direct

Name: irp [H]

Synonym: ECED1_2253

Alternate gene names: 218689980

Gene position: 2190874-2192451 (Clockwise)

Preceding gene: 218689979

Following gene: 218689981

Centisome position: 42.05

GC content: 58.62

Gene sequence:

>1578_bases
ATGAATTCTTCCTTTAAATCTCTGATTGAACAGTATCCCTTACCCATTGCCGAACAGTTGCGCCACTGGGCGGCCCGTTA
TGCCTCGCGAATTGCCGTCGTTGATGCAAAGGGGTCGTTAACCTACAGCGCGCTTGATGCACAAGTTGACGAACTTGCCG
CAGGTCTGTCATCACTGGGTTTGCGTTCGGGGGAGCATGTAATTGTGCAGCTTCCCAACGACAACGCGTTTGTTACCCTG
CTGTTCTCCTTGTTAAGACTGGGCGTTATCCCCGTGCTGGCGATGCCCTCGCAACGGGCGCTGGATATCGACGCGCTGAT
TGAGCTGGCGCAACCCGTCGCTTACGTTATTCACGGGGAAAACCACGCAGAGCTGGCCCGACAGATGGCGCACAAACACG
CCTGCTTGCGTCATGTTCTGGTCGCTGGAGAGACCGTGAGCGACGATTTTACGCCGCTCTTCTCCCTTCACGGTGAGCGA
CAGGCATGGCCGCAGCCTGATGTTTCCGCCACCGCGTTGTTGTTGCTCTCAGGCGGCACAACCGGCACGCCCAAACTCAT
CCCGCGCCGACATGCCGACTATAGCTATAACTTCAGCGCTTCTGCTGAACTGTGCGGCATCAGCCAACAGAGCGTGTATC
TCGCCGTCCTCCCGGTGGCGCATAACTTTCCGCTGGCCTGCCCCGGTATTCTGGGAACGCTTGCCTGCGGCGGAAAAGTG
GTGCTGACCGACAGCGCCAGCTGTGATGAGGTGATGCCTTTAATCGCGCAGGAAAGAGTGACTCACGTCGCCCTGGTTCC
GGCGCTGGCGCAATTATGGGTGCAGGCCAGGGAGTGGGAAGACAGCGACCTTTCGTCGCTGCGCGTCATTCAGGCAGGCG
GCGCCCGGCTCGACCCGACGCTTGCTGAGCAGGTTATCGCCACCTTTGACTGTACCCTGCAACAGGTTTTCGGTATGGCG
GAAGGCCTGCTCTGTTTTACCCGGCTGGACGATCCGCATGCCACCATTCTCCACAGCCAGGGGCGCCCGTTGTCCCCTCT
GGATGAAATCCGCATCGTTGATCAAGACGAGAACGACGTCGCGCCGGGCGAAACCGGGCAATTGTTAACGCGCGGCCCTT
ATACCATTTCGGGCTATTACCGCGCCCCTGCCCACAACGCGCAGGCCTTTACCGCGCAAGGGTTTTACCGCACAGGCGAC
AATGTCAGGCTGGATGAGGTAGGGAACCTGCACGTTGAGGGACGCATAAAAGAGCAGATCAACCGCGCCGGAGAAAAAAT
AGCCGCGGCTGAAGTGGAATCGGCACTGCTGCGTTTAGCGGAAGTGCAAGATTGCGCGGTGGTCGCCGCGCCGGACACGC
TGCTTGGCGAGCGGATTTGCGCGTTTATCATCGCGCAGCAGGTGCCAACTGACTATCAGCAGTTGCGTCAACAACTGACC
CGTATGGGGCTCAGCGCGTGGAAAATTCCTGACCAAATCGAGTTTCTGGACCACTGGCCGCTCACCGCCGTCGGCAAGAT
AGACAAAAAACGCCTGACGGCTCTCGCCGTCGACCATTATCGCCATTCTGCCCAATAA

Upstream 100 bases:

>100_bases
AGCCCGGTCCTTTTTTACGCAGATTGTCCGCCATTTTCCCCACGCATTTTCTGCAATGACCGCGTTGCAAAAACAGCCCA
GTACTTCAGAAAGGTGACGC

Downstream 100 bases:

>100_bases
GCGCAAACCGACCCGAAACAGGTTGAAATAAACCCGTTTCGGGTAGCACCACTATTAGAAATAGTTATCATTTTCAATTC
ACCATTGTCGGTATTTTTGG

Product: salicyl-AMP ligase YbtE

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFSLLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDHYRHSAQ

Sequences:

>Translated_525_residues
MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFSLLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDHYRHSAQ
>Mature_525_residues
MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFSLLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDHYRHSAQ

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=510, Percent_Identity=25.2941176470588, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=23.4082397003745, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=23.4082397003745, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI38505220, Length=407, Percent_Identity=24.5700245700246, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI115511026, Length=478, Percent_Identity=23.8493723849372, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=470, Percent_Identity=23.8297872340426, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=23.1060606060606, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42544132, Length=359, Percent_Identity=22.8412256267409, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=23.1143552311436, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=23.3716475095785, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=42.8007889546351, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=27.5193798449612, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788107, Length=533, Percent_Identity=25.328330206379, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI221142682, Length=484, Percent_Identity=23.9669421487603, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=26.2857142857143, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786529, Length=526, Percent_Identity=23.7642585551331, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790505, Length=371, Percent_Identity=24.2587601078167, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=533, Percent_Identity=27.9549718574109, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=547, Percent_Identity=24.4972577696526, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=541, Percent_Identity=26.6173752310536, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=23.960396039604, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=23.827392120075, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=23.827392120075, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=23.827392120075, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=376, Percent_Identity=25.7978723404255, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=352, Percent_Identity=27.8409090909091, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=371, Percent_Identity=27.4932614555256, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=21.5139442231076, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=352, Percent_Identity=26.1363636363636, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=243, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=535, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=535, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=25.6130790190736, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=535, Percent_Identity=22.0560747663551, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=132, Percent_Identity=28.030303030303, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355181, Length=509, Percent_Identity=24.1650294695481, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18859661, Length=376, Percent_Identity=25.2659574468085, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922652, Length=515, Percent_Identity=21.9417475728155, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=21.7647058823529, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=362, Percent_Identity=23.2044198895028, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI62472339, Length=542, Percent_Identity=22.8782287822878, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667955, Length=542, Percent_Identity=22.8782287822878, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=480, Percent_Identity=22.2916666666667, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=476, Percent_Identity=22.4789915966387, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130357, Length=520, Percent_Identity=22.1153846153846, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648260, Length=351, Percent_Identity=21.9373219373219, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 57220; Mature: 57220

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
LRSGEHVIVQLPNDNAFVTLLFSLLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECC
NHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGERQAWPQPDVSATALLLLSGGT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCC
TGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCEE
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCHH
LAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDV
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCCHHHEEEEECCCCCC
APGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGDNVRLDEVGNLHVEGRIKEQI
CCCCCCCEEECCCEEEEEEEECCCCCCCEEEECCEEECCCCEEECCCCCEEECCHHHHHH
NRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDHYRHSAQ
HCCCHHCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
LRSGEHVIVQLPNDNAFVTLLFSLLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECC
NHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGERQAWPQPDVSATALLLLSGGT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCC
TGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCEE
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCHH
LAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDV
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCCHHHEEEEECCCCCC
APGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGDNVRLDEVGNLHVEGRIKEQI
CCCCCCCEEECCCEEEEEEEECCCCCCCEEEECCEEECCCCEEECCCCCEEECCHHHHHH
NRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDHYRHSAQ
HCCCHHCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]