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The map label for this gene is rusA [H]

Identifier: 218689845

GI number: 218689845

Start: 2058408

End: 2058803

Strand: Reverse

Name: rusA [H]

Synonym: ECED1_2115

Alternate gene names: 218689845

Gene position: 2058803-2058408 (Counterclockwise)

Preceding gene: 218689846

Following gene: 218689844

Centisome position: 39.52

GC content: 54.55

Gene sequence:

>396_bases
ATGAAGCTAATACTGCCTTTTCCGCCCAGCGTGAACACGTACTGGCGACACCCCAACAAAGGGGCGTTTGCTGGTAAGAG
CCTGATAAGCGCGGCGGGGCGAAAATTCCAGAGCGCGGCGTGTGCAGCAATAGTTGAGCAGTTACGTCGTCTGCCGAAAC
CAACGTCGGCACCTGCTTCAGTGGAGATCGTGTTGTTTCCTCCGGATAACCGGATCCGCGATCTGGACAACTATAACAAG
GCGCTGTTTGACGCCCTGACCCACGCGGGTGTGTGGGAAGACGACAGTCAGGTGAAAAGAATGCTGGTGGAGTGGGGACC
GGTTATCCCGGAGGGGAAGGTCGAGATCACTATCAGTAAGTACGAAAAAGCGAGTTGCAAATTAGCAACTCGGTAA

Upstream 100 bases:

>100_bases
ACGACAGCGAGGCGGTCGGGATTATCCGCGCCCTGCTTGCCGGTGAGGCAAACGCCAGGCTGCGTGCAGCCCACTGGTTA
CATGAGAGGGGCCTGAAAGT

Downstream 100 bases:

>100_bases
CGGAATTGAGCAACACCCTAAATTTGGGTATTACCTCGTTAAAGATACTGTATTTATGAACAGTGTATCCTTGATAACTA
TTAAAAATCGCAGTAAGTTC

Product: putative Crossover junction endodeoxyribonuclease

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAIVEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNK
ALFDALTHAGVWEDDSQVKRMLVEWGPVIPEGKVEITISKYEKASCKLATR

Sequences:

>Translated_131_residues
MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAIVEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNK
ALFDALTHAGVWEDDSQVKRMLVEWGPVIPEGKVEITISKYEKASCKLATR
>Mature_131_residues
MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAIVEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNK
ALFDALTHAGVWEDDSQVKRMLVEWGPVIPEGKVEITISKYEKASCKLATR

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=118, Percent_Identity=38.9830508474576, Blast_Score=80, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14562; Mature: 14562

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAIVEQLRRLPKPTSAPAS
CEEEECCCCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCE
VEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRMLVEWGPVIPEGKVEITISK
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEC
YEKASCKLATR
CCCCCEEECCC
>Mature Secondary Structure
MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAIVEQLRRLPKPTSAPAS
CEEEECCCCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCE
VEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRMLVEWGPVIPEGKVEITISK
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEC
YEKASCKLATR
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA