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The map label for this gene is ynfL [H]

Identifier: 218689515

GI number: 218689515

Start: 1734584

End: 1735477

Strand: Reverse

Name: ynfL [H]

Synonym: ECED1_1764

Alternate gene names: 218689515

Gene position: 1735477-1734584 (Counterclockwise)

Preceding gene: 218689519

Following gene: 218689514

Centisome position: 33.31

GC content: 51.9

Gene sequence:

>894_bases
ATGAATATTGAACTTCGTCATCTGCGTTACTTTGTCGCTGTTGCAGAAGAGCTGCATTTCGGGCGTGCCGCTGCCCGCCT
GAATATTTCGCAACCACCTCTAAGTCAGCAGATTCAGGCGCTGGAGCAACAAATTGGTGCCCGACTGCTGGCACGGACCA
ATCGCAGTGTATTGCTGACGGAAGCAGGAAAACAGTTTCTTGTAGATAGTCGGCAAATCCTGTCTATGGTGGATGATGCT
GCCGCTCGCGCTGAAAGGCTGCATCAGGGCGAAGCGGGGGAGTTGCGCATTGGTTTTACTTCGTCCGCTCCTTTTATTCG
GGCGGTGTCCGATACGTTATCGCTGTTTCGCCGTGATTATCCCGATGTCCATTTACAAACCCGCGAAATGAACACTCGCG
AGCAAATCGCTCCGCTCATTGAAGGAACGCTGGATATGGGATTGCTGCGTAACACAGAGTTACCGGAGACGCTTGAACAC
GCAGTCATCGTCCATGAACCGCTTATGGCGATGATCCCGCACGATCATCCCCTGGCAAATAACCCGAGTGTAACGCTGGC
TGAACTGGCGAAAGAACCCTTTGTCTTTTTTGATCCGCACGTCGGAACAGGGCTGTATGACGATATTCTCGGGCTGATGA
GACGTTACAATTTGACGCCCGTCATCACTCAGGAGGTGGGTGAGGCAATGACCATCATCGGTCTGGTTTCCGCCGGTCTG
GGTGTTTCAATTTTGCCTGCGTCATTTAAACGTGTTCAGCTCAACGAAATGCGCTGGGTGCCGATTGCTGAAGAGGATGC
GGTTTCTGAAATGTGGTTGGTCTGGCCGAAACATCATGAACAAAGTCCGGCTGCGCGTAACTTTCGTATTCATCTGCTGA
ATGCTCTCAGGTGA

Upstream 100 bases:

>100_bases
TCCTTGCTAAATATGCCTGTGGATCAGGCTTATGAATAGATTAGGAAAATCGAATTGTTCTGTCTAATATATTAATAATC
TCAAATAAGATGTTTTAAAT

Downstream 100 bases:

>100_bases
GGGAAATTTCAGCGAAAAAGCCCGAAAAATGTGCTGTTAATCACATGCCTAAGTAAAAATTTGACGACACGTATTGAAGT
GCTTCACCATAGCCTACAGA

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTELPETLEH
AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Sequences:

>Translated_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTELPETLEH
AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
>Mature_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTELPETLEH
AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=97.979797979798, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=36.3636363636364, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=38.1322957198444, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=31.8548387096774, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=29.1497975708502, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=26.7796610169492, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=29.7945205479452, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=32.8205128205128, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=28.6290322580645, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=26.7657992565056, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=44.6808510638298, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=24.5059288537549, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=286, Percent_Identity=24.1258741258741, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786713, Length=258, Percent_Identity=27.5193798449612, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=25.0836120401338, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789506, Length=282, Percent_Identity=22.6950354609929, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=33.0769230769231, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=239, Percent_Identity=26.3598326359833, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=33.5570469798658, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=251, Percent_Identity=26.2948207171315, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082132, Length=301, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33390; Mature: 33390

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
EAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTELPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCC
NPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
EAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTELPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCC
NPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]