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The map label for this gene is ydfI [H]

Identifier: 218689431

GI number: 218689431

Start: 1656076

End: 1657536

Strand: Reverse

Name: ydfI [H]

Synonym: ECED1_1670

Alternate gene names: 218689431

Gene position: 1657536-1656076 (Counterclockwise)

Preceding gene: 218689432

Following gene: 218689427

Centisome position: 31.82

GC content: 49.28

Gene sequence:

>1461_bases
ATGGGAAATCATTTGTTATCAGCAAAAGCGACGCTCCCTGTTTATGATCGTAATAACCTGGCCCCAAGAATTGTTCATTT
AGGCTTTGGTGCATTTCACCGTGCACATCAGGGCGTGTATGCCGATATTCTTGCTACGGAACATTTCAGTGACTGGGGAT
ATTATGAGGTCAACTTAATCGGCGGCGAACAGCAAATTGCCGATTTACAACAGCAAGATAATCTTTATACCGTTGCGGAA
ATGTCGGCTGATGCGTGGACGGCTCGCGTCGTTGGCGTCGTTAAAAAAGCCTTGCACGTACAAATAGATGGCTTAGAAAC
CGTGTTGGCTGCGATGTGTGAACCACAAATCGCGATTGTCTCTCTGACAATCACCGAAAAAGGGTATTTCCACTCTCCGG
CGACCGGACAGTTAATGCTTGATCACCTGATGGTCGTTGCCGACGTGCAAAATCCCCACCAGCCGAAAACTGCAACAGGG
GTGATTGTTGAGGCGCTGGCTCGCCGTAAAGCGGCAGGACTTCCCGCATTTACCGTCATGTCATGTGACAACATGCCAGA
AAACGGTCATGTTATGCGTGACGTTGTCACTTCCTATGCGCAAGCCATTGATGTAAAACTGGCACAATGGATCGAAGATA
ACGTGACTTTCCCATCAACAATGGTGGACCGTATTGTGCCCGCAGTGACAGAGGATACGCTGGCGAAAATCGAACAACTT
ACCGGTGTGCGCGATGCGGCAGGCGTTGCCTGTGAACCTTTCCGCCAGTGGGTAATAGAAGATAACTTTGTTGCCGGACG
TCCGGAATGGGAAAAAGCGGGAGCTGAACTGGTTAGCGATGTGCTGCCTTATGAAGAAATGAAACTGCGTATGCTTAACG
GCAGTCATTCATTCCTGGCGTATCTGGGCTATCTTGCCGGATATCAGCACATTAATGACTGCATGGAGGATGAACATTAT
CGTCATGCGGCGTATACCTTAATGTTGCAGGAACAAGCGCCGACGCTGAAAGTGCAGGGCGTTGATTTGCAAGATTACGC
TAACCGATTAATTGAACGCTATAGCAACCCGGCGTTACGTCATCGAACCTGGCAGATTGCGATGGATGGCAGCCAGAAAT
TGCCACAGCGGATGTTGGATTCTGTTCGCTGGCATCTGGCGCATGACAGCAAGTTCGATCTGCTGGCGCTGGGCGTCGCG
GGTTGGATGCGTTATGTCGGTGGTGTTGATGAACAGGGAAATCCGATAGAAATCAGTGATCCACTGTTACCTGTTATTCA
GAAGGCAGTACAAAGTAGTGCTGAAGGGACAGCGCGCGTCCAGTCACTGCTGGCAATTAAGGCGATCTTTGGTGATGATT
TACCAGGCAATAGTTTGTTTACGACAAAAGTAACGGAAGCGTACTTGTCTTTATTAGCGCATGGCGCGAAAGCGACCGTG
GCGAAATATTCCGTGAAGTAA

Upstream 100 bases:

>100_bases
AGAAGTCTGTAAATCCTCAATCCCTTCTCTTACCGGAGAGGGGATTTTTATTCATATAACAAAACATGTAGCTTGCTATA
TTTAATTTTAAGGAATTGTT

Downstream 100 bases:

>100_bases
AAAAAGCAATGCCGCCATCGGCGGCATTCACACGCTTAGTTCATGCCGAGACGAATCAGGTCCAGTGGTGCCAGTTTCTC
TTCACAACCTTCAACAACTA

Product: putative mannonate dehydrogenase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHLMVVADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYTLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLPGNSLFTTKVTEAYLSLLAHGAKATV
AKYSVK

Sequences:

>Translated_486_residues
MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHLMVVADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYTLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLPGNSLFTTKVTEAYLSLLAHGAKATV
AKYSVK
>Mature_485_residues
GNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEM
SADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHLMVVADVQNPHQPKTATGV
IVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLT
GVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYR
HAAYTLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAG
WMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLPGNSLFTTKVTEAYLSLLAHGAKATVA
KYSVK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=486, Percent_Identity=96.9135802469136, Blast_Score=976, Evalue=0.0,
Organism=Escherichia coli, GI1790779, Length=472, Percent_Identity=58.0508474576271, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1788497, Length=464, Percent_Identity=54.5258620689655, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI48994885, Length=367, Percent_Identity=25.8855585831063, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790028, Length=267, Percent_Identity=26.9662921348315, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324401, Length=485, Percent_Identity=40.2061855670103, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6320765, Length=485, Percent_Identity=40.2061855670103, Blast_Score=358, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53719; Mature: 53588

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLI
CCCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHLMVVADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TGVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
CCCCHHCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHH
HRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECC
PLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLPGNSLFTTKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
AKYSVK
HEECCC
>Mature Secondary Structure 
GNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLI
CCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHLMVVADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TGVRDAAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
CCCCHHCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHH
HRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECC
PLLPVIQKAVQSSAEGTARVQSLLAIKAIFGDDLPGNSLFTTKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
AKYSVK
HEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]