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The map label for this gene is narY [H]

Identifier: 218689390

GI number: 218689390

Start: 1599224

End: 1600768

Strand: Reverse

Name: narY [H]

Synonym: ECED1_1620

Alternate gene names: 218689390

Gene position: 1600768-1599224 (Counterclockwise)

Preceding gene: 218689391

Following gene: 218689389

Centisome position: 30.73

GC content: 53.79

Gene sequence:

>1545_bases
ATGAAAATACGTTCGCAAGTCGGCATGGTGCTTAATCTCGATAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAA
AAACGTCTGGACCGGACGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGCATTGGTTATCCGA
AAAACTGGGAAGATCAGGATGAGTGGCAAGGTGGCTGGGTGCGTGATGTGAATGGCAAGATACGCCCGCGTCTGGGCAGC
AAGATGGGCGTAATAACCAAAATTTTCGCCAACCCGGTAGTGCCGCAGATTGACGATTACTACGAACCTTTCACCTTCGA
CTACGAACATTTGCATAGCGCGCCGGAAGGCAAACATATTCCCACCGCCCGTCCGCGTTCACTGATTGACGGCAAACGGA
TGGACAAAGTGATCTGGGGCCCAAACTGGGAAGAACTGCTGGGCGGCGAATTTGAAAAACGAGCCCGCGACCGCAACTTC
GAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTATCTGCCGCGCTTGTGCGAACACTGCCT
TAACCCCAGTTGTGTAGCGACCTGCCCCAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATCGATCAGG
ATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGCCCATACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCA
GAAAAATGTATATTTTGTTATCCGCGAATTGAGTCCGGTCAACCGACCGTGTGCTCCGAAACCTGCGTGGGTCGCATCCG
GTATCTGGGCGTGCTGCTTTACGACGCCGACCGCATTGAGGAAGCGGCAAGCACCGAGCGCGAAGTTGACCTCTATGAGC
GCCAGTGCGAAGTGTTCCTCGATCCACACGATCCCTCAGTGATCGAGGAAGCCCTGAAACAAGGTATTCCACAAAACGTG
ATTGACGCTGCCCAGCGTTCGCCTGTCTACAAAATGGCGATGGACTGGAAACTGGCGCTACCGCTGCACCCTGAATATCG
CACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTCCTACGCTGATGCGGGAGGTTTGCCGAAAAGCG
AAGGCATGCTGCCCGCCATCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGTGATACCGGT
CCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAGGCGTTACTGATAC
TCGTGCCATCGACGAAGTAGGTCTGAGCGTCGCCCAGGTCGAAGAGATGTATCGTTACCTCGCCATTGCCAACTATGAAG
ATCGTTTCGTCATCCCGACAAGCCATCGGGAAATGGCGGGCGATGCCTTCGCAGAACGCAACGGCTGCGGTTTTACCTTT
GGCGACGGTTGCCACGGTTCGGACAGCAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATCAACATCACCGAAGT
GCGCGACAAAGCGGAGGGCGAATAA

Upstream 100 bases:

>100_bases
CCGTCGGCTCGAACCGCGACGAATTCATTATGATCCGCAAAATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGAT
CAGGTTCAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAGATCCTCAAAGTGATCGGCCTGTTGATGGAGTACCCAGACGAGTTGTTGTGGGAGTGTAAGGACGACGCGCTGGCG
CTTATCCGCCGCGACGCGCC

Product: nitrate reductase 2 (NRZ) subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGMLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGMLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGMLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=99.4163424124514, Blast_Score=1071, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=78.6561264822134, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=41.5841584158416, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58550; Mature: 58550

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGMLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGMLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]