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The map label for this gene is topA [H]

Identifier: 218689265

GI number: 218689265

Start: 1451388

End: 1453985

Strand: Direct

Name: topA [H]

Synonym: ECED1_1481

Alternate gene names: 218689265

Gene position: 1451388-1453985 (Clockwise)

Preceding gene: 218689264

Following gene: 218689266

Centisome position: 27.86

GC content: 51.81

Gene sequence:

>2598_bases
ATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGT
GAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCA
AGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAG
GCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTA
TCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTGGTGGTGATGATGCGCGCT
ATAGACGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAACTGAATATTGAT
CGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTTCTATGGAAAAAGAT
CGCTCGTGGTCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTCCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGT
TCGTACCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTGCAGGTGACTCAT
CAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAG
CGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCA
GCACCCGTCTTGGTTTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATG
CGTACCGACTCTACCAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATA
TCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCA
ATGTGATGGCGGAATCGTTGAAGGATATGGAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTAGCC
TGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCG
TATTTTGCGCTTTGATGGCTGGACGAAAGTGATGCCTGCACTGCGTAAAGGCGATGAAGATCGTATCTTACCGGCAGTCG
ATAAAGGCGATGCTCTGACGCTCGTTGAACTGACACCAGCCCAGCACTTTACCAAGCCTCCAGCCCGTTTCAGTGAAGCA
TCGCTGGTCAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCTTCTACCTATGCGTCGATCATTTCGACTATTCAGGATCG
TGGCTATGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTGGAAGAGAATT
TCCGCGAGTTAATGAACTACGATTTCACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGG
AAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGGGGTAT
GCGCCCGAACCAGATGGTTCTGACCAGCATTGACTGTCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCG
GGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAAC
GAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGAAACCAATGCGTTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCAC
GGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGA
TCGAAGAGGGTGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTG
AAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGCGA
AGTGGCACCACCGAAAGAAGATCCGGTGCCATTACCGGAGCTGCCGTGCGAAAAATCAGATGCCTATTTCGTGTTGCGTG
ACGGTGCTGCCGGTGTGTTCCTGGCGGCCAATACCTTCCCGAAATCGCGTGAAACGCGTGCGCCGCTGGTGGAGGAGCTT
TATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGAC
CATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCAT
TTTATGTTGATGGCAAATGGGTTGAAGGGAAAAAATAA

Upstream 100 bases:

>100_bases
ATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAA
TCAAATTAGGTAAGGTGAAT

Downstream 100 bases:

>100_bases
CCTTTAATTCTGTCGGGTTTTTATAAACAAAGGGCCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAC
CTATACACTAAGGCTATAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE
AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYRRVVFNEITKNAIRQAFNKPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH
QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM
RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA
CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW
KAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN
EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK

Sequences:

>Translated_865_residues
MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE
AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYRRVVFNEITKNAIRQAFNKPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH
QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM
RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA
CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW
KAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN
EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
>Mature_864_residues
GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEA
HYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYRRVVFNEITKNAIRQAFNKPGELNIDR
VNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQ
NDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMR
TDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVAC
QMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEAS
LVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWK
AVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENE
VLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLK
MGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELY
RFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=25.2873563218391, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4507635, Length=514, Percent_Identity=27.431906614786, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=99.7687861271676, Blast_Score=1799, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=23.6794171220401, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=24.6017699115044, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=25.5445544554455, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24585251, Length=618, Percent_Identity=24.1100323624595, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24640096, Length=508, Percent_Identity=25.9842519685039, Blast_Score=122, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97421; Mature: 97290

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK
CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC
KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
WHLREVIGGDDARYRRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH
PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE
QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE
RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA
EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
DFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC
TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR
CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH
RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH
KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SSEKDGKATGWSAFYVDGKWVEGKK
CCCCCCCCCCEEEEEECCEECCCCC
>Mature Secondary Structure 
GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC
KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
WHLREVIGGDDARYRRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH
PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE
QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE
RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA
EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
DFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC
TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR
CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH
RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH
KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SSEKDGKATGWSAFYVDGKWVEGKK
CCCCCCCCCCEEEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]