Click here to switch to the map view.

The map label for this gene is proC [H]

Identifier: 218688249

GI number: 218688249

Start: 425803

End: 426612

Strand: Reverse

Name: proC [H]

Synonym: ECED1_0409

Alternate gene names: 218688249

Gene position: 426612-425803 (Counterclockwise)

Preceding gene: 218688256

Following gene: 218688244

Centisome position: 8.19

GC content: 54.44

Gene sequence:

>810_bases
ATGGAAAAGAAAATCGGTTTTATTGGCTGCGGCAATATGGGAAAAGCCATTCTCAGCGGTCTGATTGCCAGCGGTCAGGT
GTTTCCAGGGCAAATCTGGGTATATACCCCCTCCCCGGATAAAGTCACCGCCCTGCATGACCAGTTCGGCATCAACGCCG
CAGAATCGGCGCAAGAAGTAGCGCAAATCGCCGACATCATTTTTGCCGCCGTTAAACCAGGCATCATGATTAAAGTGCTT
AGCGAAATCACCTCCAGCCTGAATAAAGACTCTCTGGTCGTTTCTATTGCTGCAGGTATCACGCTCGACCAGCTTGCCCG
CGCGCTGGGCCATGACCGGAAAATTATCCGCGCCATGCCGAACACTCCCGCGCTGGTTAATGCCGGGATGACCTCCGTAA
CGCCAAACGCGCTGGTAACCCCAGAAGATACCGCTGATGTGCTGAATATTTTCCGCTGCTTTGGCGAAGCGGAAGTGATT
GCTGAGCCGATGATCCACCCGGTGGTCGGTGTGAGCGGTTCTTCGCCAGCCTACGTCTTTATGTTTATCGAAGCGATGGC
CGACGCCGCCGTGCTGGGCGGGATGCCACGCGCCCAGGCGTATAAATTTGCCGCTCAGGCGGTAATGGGCTCCGCAAAAA
TGGTGCTGGAAACGGGAGAACATCCGGGAACACTGAAAGATATGGTCTGCTCACCGGGAGGCACCACCATTGAGGCGGTA
CGCGTACTGGAAGAGAAAGGCTTCCGTGCCGCAGTGATCGAAGCGATGACGAAGTGTATGGAAAAATCAGAAAAACTCAG
CAAATCCTGA

Upstream 100 bases:

>100_bases
TAATCCTCTATTGTGTCGCGCTTTTGCCTTCCGGCATAGTTCTGTTTATGCTTCTGCCAGCGATTATCAAAACAATAAAT
TTCACGGCAGGAGTGAGGCA

Downstream 100 bases:

>100_bases
TGGCTTTTGCCGGACGTCAGGCCGCCACTTCGGTGCGGTTACGTCCGGCTTTCTTTGCTTTGTAGAGCGCCAAATCTGCC
GATTTCAACCACTCGCGATA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEKKIGFIGCGNMGKAILSGLIASGQVFPGQIWVYTPSPDKVTALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGTLKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Sequences:

>Translated_269_residues
MEKKIGFIGCGNMGKAILSGLIASGQVFPGQIWVYTPSPDKVTALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGTLKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
>Mature_269_residues
MEKKIGFIGCGNMGKAILSGLIASGQVFPGQIWVYTPSPDKVTALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGTLKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361454, Length=266, Percent_Identity=37.593984962406, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI24797097, Length=266, Percent_Identity=37.593984962406, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI24797095, Length=266, Percent_Identity=37.593984962406, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI198041662, Length=266, Percent_Identity=34.2105263157895, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=98.1412639405204, Blast_Score=539, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17569021, Length=264, Percent_Identity=40.530303030303, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17540664, Length=297, Percent_Identity=35.016835016835, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320861, Length=272, Percent_Identity=29.7794117647059, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=38.6281588447653, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=39.622641509434, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=42.0118343195266, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 28283; Mature: 28283

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKIGFIGCGNMGKAILSGLIASGQVFPGQIWVYTPSPDKVTALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGTLKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKIGFIGCGNMGKAILSGLIASGQVFPGQIWVYTPSPDKVTALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGTLKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6296787; 9278503 [H]