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The map label for this gene is gloB [H]

Identifier: 218688082

GI number: 218688082

Start: 238973

End: 239728

Strand: Reverse

Name: gloB [H]

Synonym: ECED1_0217

Alternate gene names: 218688082

Gene position: 239728-238973 (Counterclockwise)

Preceding gene: 218688084

Following gene: 218688081

Centisome position: 4.6

GC content: 39.95

Gene sequence:

>756_bases
ATGAATCTTAACAGTATTCCCGCCTTTGATGACAATTACATCTGGGTTTTGAATGATGAAGCAGGTCGCTGTCTGATTGT
CGATCCCGGGGACGCAGAGCCAGTATTAAACGCCATTTCCGCCAATAACTGGCAACCGGAGGCCATATTTCTCACCCACC
ATCACCACGATCACGTTGGAGGCGTAAAAGAACTGGTGGAAAAGTTTCCGCAAATTGTGGTGTATGGACCACAAGAGACA
CAAGATAAGGGAACAACACAGGTAGTCAAAGATGGCGAAACTGCCTTCGTTTTGGGGCATGAATTTAGTGTAATTGCCAC
ACCGGGTCACACTTTAGGACATATCTGTTACTTCAGTAAACCTTATCTATTTTGCGGCGACACGCTGTTTTCTGGTGGGT
GTGGTCGGTTGTTTGAAGGGACACCATCACAAATGTATCAATCACTTAAAAAGTTAAGTGCGTTACCTGACGATACATTG
GTATGTTGTGCTCATGAATATACCTTATCAAATATGAAGTTTGCTTTGAGTATTCTTCCGCACGATTTGTCCATAAATGA
TTATTATCGTAAAGTTAAGGAGTTACGGGCAAAAAATCAAATAACACTACCCGTAATTCTGAAAAATGAGCGGCAAATTA
ATGTTTTTTTAAGAACGGAAAATATTGATTTAATTAATGTAATTAATGAAGAAACATTATTGCAACAACCTGAAGAACGT
TTTGCATGGTTAAGGTCAAAGAAAGATAGGTTCTGA

Upstream 100 bases:

>100_bases
CTCCCCAGGGCAAATCGCCCCAGCAATCAGGAGCCACGACAGTTTGAGGGACTCTTGCCGGTTTCATCACAACCTTCCGT
TTCACACTGAGAGGTAATCT

Downstream 100 bases:

>100_bases
TAAAACTTTCTTGTCATCGGCTCCGTTCGCCGTTATGATCGGTCGTCTTTTAAGCAACTATTGACACACACATGAAGGCA
AAAGCGATATTACTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTENIDLINVINEETLLQQPEER
FAWLRSKKDRF

Sequences:

>Translated_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTENIDLINVINEETLLQQPEER
FAWLRSKKDRF
>Mature_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTENIDLINVINEETLLQQPEER
FAWLRSKKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=33.8461538461538, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=33.8461538461538, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI14150041, Length=227, Percent_Identity=35.2422907488987, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI116642887, Length=232, Percent_Identity=34.9137931034483, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI21703352, Length=232, Percent_Identity=34.9137931034483, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=36.8686868686869, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=98.804780876494, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=30.0518134715026, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=227, Percent_Identity=32.15859030837, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320478, Length=231, Percent_Identity=32.9004329004329, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=231, Percent_Identity=29.8701298701299, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=36.0153256704981, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=36.0153256704981, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=36.0153256704981, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28476; Mature: 28476

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTENIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEEECCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC
>Mature Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTENIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEEECCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA