Click here to switch to the map view.

The map label for this gene is relE [H]

Identifier: 218551533

GI number: 218551533

Start: 4427457

End: 4427831

Strand: Direct

Name: relE [H]

Synonym: EFER_4317

Alternate gene names: 218551533

Gene position: 4427457-4427831 (Clockwise)

Preceding gene: 218551532

Following gene: 218551535

Centisome position: 96.49

GC content: 49.07

Gene sequence:

>375_bases
GTGATAGTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGAT
GAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAAAA
AAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTAAG
CTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAGAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGACTAA

Upstream 100 bases:

>100_bases
GTGCTGGAAAAATTAAATATCACGCCTACTGAAGCAGTGCGGCAGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCC
GGTAAAAACGCTCACTGTCA

Downstream 100 bases:

>100_bases
CGTAAATGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGTCTTTTAGATCCTGCATAAATTTGCCGTC
GACCAGCACGTTAATCAAAT

Product: RelE protein; cytotoxic translational repressor of toxin-antitoxin stability system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MIVKKPCYALSGNDWPTHRRPLRSDWMNYELAFDPRALKEWQKLGVTVREQFKKKLGDVLKRPRNPSAKLRDLPDCYKIK
LRTLGYRRVYQVNDKELLVLVIAIGKRERTQPFMKTRQNGWMSD

Sequences:

>Translated_124_residues
MIVKKPCYALSGNDWPTHRRPLRSDWMNYELAFDPRALKEWQKLGVTVREQFKKKLGDVLKRPRNPSAKLRDLPDCYKIK
LRTLGYRRVYQVNDKELLVLVIAIGKRERTQPFMKTRQNGWMSD
>Mature_124_residues
MIVKKPCYALSGNDWPTHRRPLRSDWMNYELAFDPRALKEWQKLGVTVREQFKKKLGDVLKRPRNPSAKLRDLPDCYKIK
LRTLGYRRVYQVNDKELLVLVIAIGKRERTQPFMKTRQNGWMSD

Specific function: Inhibits translation during amino acid starvation. Acts by cleaving mRNA with high codon specificity in the ribosomal A site. UGA is cleaved with fast rate while UAA and UGA are cleaved with intermediate and slow rate, respectively. mRNA cleavage can also

COG id: COG2026

COG function: function code JD; Cytotoxic translational repressor of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787846, Length=87, Percent_Identity=63.2183908045977, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007712
- InterPro:   IPR012753 [H]

Pfam domain/function: PF05016 Plasmid_stabil [H]

EC number: NA

Molecular weight: Translated: 14806; Mature: 14806

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKKPCYALSGNDWPTHRRPLRSDWMNYELAFDPRALKEWQKLGVTVREQFKKKLGDVL
CCCCCCCEEECCCCCCCCCCCCHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
KRPRNPSAKLRDLPDCYKIKLRTLGYRRVYQVNDKELLVLVIAIGKRERTQPFMKTRQNG
HCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCC
WMSD
CCCC
>Mature Secondary Structure
MIVKKPCYALSGNDWPTHRRPLRSDWMNYELAFDPRALKEWQKLGVTVREQFKKKLGDVL
CCCCCCCEEECCCCCCCCCCCCHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
KRPRNPSAKLRDLPDCYKIKLRTLGYRRVYQVNDKELLVLVIAIGKRERTQPFMKTRQNG
HCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCC
WMSD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2990907; 9097039; 9278503; 9767574; 12526800 [H]