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The map label for this gene is rpiR [H]

Identifier: 218551498

GI number: 218551498

Start: 4383788

End: 4384678

Strand: Direct

Name: rpiR [H]

Synonym: EFER_4281

Alternate gene names: 218551498

Gene position: 4383788-4384678 (Clockwise)

Preceding gene: 218551495

Following gene: 218551499

Centisome position: 95.53

GC content: 47.03

Gene sequence:

>891_bases
ATGAGCCAGTCAGAGTTTGATTCAGGGCTTCCTGAGGGAATCGGGCTTGCCCCCTACCTGCGAATGAAGCAGGAAGGCAT
GACAGAAAACGAAAGTCGGATCGTGGAGTGGTTGCTGAAACCAGGCAATCTTAGTAGTGCTCCAGCGATAAAAGATGTTG
CGGAAGCATTAGCCGTCTCCGAGGCGATGATCGTAAAGGTCTCAAAATTATTAGGGTTTAGCGGCTTTCGTAACTTGCGT
AGTGCGCTGGAAGATTATTTTTCTCAGTCCGAGCAGGTACTTCCCAGTGAACTGGCTTTTGATGAAGCGCCACAGGATGT
CGTGAACAAGGTGTTCAACATCACGTTACGCACCATTATGGAAGGCCAGTCGATTGTCAATGTCGATGAGATTCACCGGG
CCGCGCGTTTCTTTTATCAGGCAAAACAACGCGATCTCTATGGCGCTGGCGGTTCAAACGCTATTTGTGCGGATATTCAG
CACAAGTTTTTACGTATTGGTGTGCGCTGTCAGGCCTATCCTGATGCTCACATCATGATGATGTCCGCTTCGTTGTTACA
AGAAGGCGATGTGGTGCTGGTAGTGACCCACTCAGGGCGAACCAGTGATGTGAAGGCGGCTGTAGAACTGGCGAAAAAGA
ATGGGGCAAAAATTATTTGTATCACTCATAGCTATCATTCTCCGATAGCGAAGTTGGCTGATTATATTATTTGCTCCCCG
GCACCTGAAACACCGTTATTAGGTAGAAACGCCTCTGCCCGCATTTTACAACTTACTTTGCTGGATGCTTTTTTTGTTTC
TGTTGCCCAGCTTAATATTGAACAGGCCAATAAAAATATGCAAAAAACGGGCGCAATTGTTGATTTCTTTTCACCAGGTG
CGCTGAAGTAA

Upstream 100 bases:

>100_bases
AAACTTTATGAATTTAGCGGTTTTGTGAAGTTGTCAGCATCTTTTCTGTTTTTGCTGTGGTGTTATAGTGGCGTCTTTAT
TTCATGGACAAGAGAACGCG

Downstream 100 bases:

>100_bases
AAAAGTAATCCCGCAATCGCGGGATTACCACTCAACCCTACACTGAGAACATTATTATGAATAAATATCTGAAATATTTC
AGCGGCGCTGCTGTGGGCTT

Product: DNA-binding transcriptional repressor RpiR

Products: NA

Alternate protein names: Als operon repressor [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSQSEFDSGLPEGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSSAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLR
SALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQAKQRDLYGAGGSNAICADIQ
HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANKNMQKTGAIVDFFSPGALK

Sequences:

>Translated_296_residues
MSQSEFDSGLPEGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSSAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLR
SALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQAKQRDLYGAGGSNAICADIQ
HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANKNMQKTGAIVDFFSPGALK
>Mature_295_residues
SQSEFDSGLPEGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSSAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRS
ALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQAKQRDLYGAGGSNAICADIQH
KFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSPA
PETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANKNMQKTGAIVDFFSPGALK

Specific function: Regulatory protein involved in rpiB gene repression. Also involved in als operon repression [H]

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI87082369, Length=296, Percent_Identity=97.972972972973, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1788767, Length=269, Percent_Identity=26.3940520446097, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788159, Length=240, Percent_Identity=25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082130, Length=274, Percent_Identity=21.1678832116788, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01418 HTH_6; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 32349; Mature: 32217

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSEFDSGLPEGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSSAPAIKDVAEALAVS
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIM
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHH
EGQSIVNVDEIHRAARFFYQAKQRDLYGAGGSNAICADIQHKFLRIGVRCQAYPDAHIMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHH
MSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEECC
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANKNMQKTGAIVDFFSPGALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
SQSEFDSGLPEGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSSAPAIKDVAEALAVS
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIM
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHH
EGQSIVNVDEIHRAARFFYQAKQRDLYGAGGSNAICADIQHKFLRIGVRCQAYPDAHIMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHH
MSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEECC
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANKNMQKTGAIVDFFSPGALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]