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The map label for this gene is napF [C]

Identifier: 218551404

GI number: 218551404

Start: 4293877

End: 4294551

Strand: Direct

Name: napF [C]

Synonym: EFER_4185

Alternate gene names: 218551404

Gene position: 4293877-4294551 (Clockwise)

Preceding gene: 218551403

Following gene: 218551410

Centisome position: 93.57

GC content: 49.19

Gene sequence:

>675_bases
GTGGGGATATCGTTATCTGGAGTTATTACAGCGCAACCCTGTCAGTGCGTTTTACGCGAAGTTGGCGCAGATTGCGACAC
TGTTTCTGCAGGATTATCAGCAGCAACAGGCACTAAATCCTCCAACCAAACGGCTCTTTCTTGGATGAGTAAGTCAGAAA
AATATTATCAGGAGTTAATGTCTCATCGGTATGTTAGCCGACGTGGGCTATTTCGGGCCTTTGTCAGCGCGGCACGCCCG
GTAACAACACCGATATTACCTGTGCACTCCTTACCTCCCGGCGCGTTACCTGATGCGCAGTTTCGTGTGCAGTGCACGCA
GTGTGATTTGTGTATTAATGCCTGTCCCATGGGCATTCTCAACCGGCATGAAGATGGCTATCCGCAATTGGTGATTGAAT
TTGCCAGTTGTGACGGCTGCGGCTTATGTATCGCTGCATGTTCTACTGAGGCATTACGTCCGCAGGCTCGTTTCGACACC
GGATTGCGTCCCGTTTTTAAAGCAAATTGTGTTAATCCGGTGCGCAGTTGCAAACAGTGTGTCGATCTATGCCCACTTCA
GGCGTGCTCAATCAACGAAAGCGGAATGCCTGTAATCGATGCTGCAATATGTAATGGTTGCGGTGAATGTTTGGTTCAGT
GTGGTTATGACGCGGTAAAACTTGAGATGGTGTGA

Upstream 100 bases:

>100_bases
CGGAAGATCAGTTTGGCCTGATGCTGCTTGCCTGTAGCGCCTTGCTGGCAGAGGGGAAAGAACGTGCAGCTAATCAATTG
CTGGAAACGCATTTGCTGCC

Downstream 100 bases:

>100_bases
GGGGCAGGGAGGAACACTCTGTGTATTAATAGATTTCACTTGATGACCTGGAAATTATTATTTTTAGGAATGAGGATTCT
TATCTGCATGGAAAATATGT

Product: ferredoxin

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGISLSGVITAQPCQCVLREVGADCDTVSAGLSAATGTKSSNQTALSWMSKSEKYYQELMSHRYVSRRGLFRAFVSAARP
VTTPILPVHSLPPGALPDAQFRVQCTQCDLCINACPMGILNRHEDGYPQLVIEFASCDGCGLCIAACSTEALRPQARFDT
GLRPVFKANCVNPVRSCKQCVDLCPLQACSINESGMPVIDAAICNGCGECLVQCGYDAVKLEMV

Sequences:

>Translated_224_residues
MGISLSGVITAQPCQCVLREVGADCDTVSAGLSAATGTKSSNQTALSWMSKSEKYYQELMSHRYVSRRGLFRAFVSAARP
VTTPILPVHSLPPGALPDAQFRVQCTQCDLCINACPMGILNRHEDGYPQLVIEFASCDGCGLCIAACSTEALRPQARFDT
GLRPVFKANCVNPVRSCKQCVDLCPLQACSINESGMPVIDAAICNGCGECLVQCGYDAVKLEMV
>Mature_223_residues
GISLSGVITAQPCQCVLREVGADCDTVSAGLSAATGTKSSNQTALSWMSKSEKYYQELMSHRYVSRRGLFRAFVSAARPV
TTPILPVHSLPPGALPDAQFRVQCTQCDLCINACPMGILNRHEDGYPQLVIEFASCDGCGLCIAACSTEALRPQARFDTG
LRPVFKANCVNPVRSCKQCVDLCPLQACSINESGMPVIDAAICNGCGECLVQCGYDAVKLEMV

Specific function: Involved In Electron Transfer. [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=138, Percent_Identity=29.7101449275362, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 23938; Mature: 23807

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00022 EGF_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
11.6 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
11.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISLSGVITAQPCQCVLREVGADCDTVSAGLSAATGTKSSNQTALSWMSKSEKYYQELM
CCCCCCCEEECCHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SHRYVSRRGLFRAFVSAARPVTTPILPVHSLPPGALPDAQFRVQCTQCDLCINACPMGIL
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHH
NRHEDGYPQLVIEFASCDGCGLCIAACSTEALRPQARFDTGLRPVFKANCVNPVRSCKQC
CCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHCCHHHHCCCCCHHHHHCCCCHHHHHHHH
VDLCPLQACSINESGMPVIDAAICNGCGECLVQCGYDAVKLEMV
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
GISLSGVITAQPCQCVLREVGADCDTVSAGLSAATGTKSSNQTALSWMSKSEKYYQELM
CCCCCCEEECCHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SHRYVSRRGLFRAFVSAARPVTTPILPVHSLPPGALPDAQFRVQCTQCDLCINACPMGIL
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHH
NRHEDGYPQLVIEFASCDGCGLCIAACSTEALRPQARFDTGLRPVFKANCVNPVRSCKQC
CCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHCCHHHHCCCCCHHHHHCCCCHHHHHHHH
VDLCPLQACSINESGMPVIDAAICNGCGECLVQCGYDAVKLEMV
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]