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The map label for this gene is yjbN [H]

Identifier: 218551359

GI number: 218551359

Start: 4238220

End: 4239215

Strand: Direct

Name: yjbN [H]

Synonym: EFER_4138

Alternate gene names: 218551359

Gene position: 4238220-4239215 (Clockwise)

Preceding gene: 218551357

Following gene: 218551360

Centisome position: 92.36

GC content: 51.91

Gene sequence:

>996_bases
ATGTCAGCAGAATCACAAACCGCTTATCCCGCTTACCGTTTCTCCATTGCTCCGATGCTCGACTGGACAGACAGGCACTG
CCGTTATTTCTTGCGTCTGCTCTCTCGTAATACGCTGCTGTATACCGAGATGGTGACCACGGGGGCGATTATTCACGGTA
AAGGTGATTACCTGGCATACAGCGAAGAAGAGCATCCGGTGGCATTGCAACTGGGTGGTAGCGATCCGGCGGCGCTGGCA
CAGTGTGCGAAACTGGCGGAAGCGCGTGGATATGATGAAATCAACCTCAATGTGGGCTGCCCGTCTGATCGCGTACAGAA
CGGTATGTTTGGTGCGTGCCTGATGGGTAACGCGCAGCTGGTGGCGGACTGCGTGAAAGCGATGCGTGATGTGGTGTCGA
TTCCGGTGACGGTGAAAACGCGTATTGGTATTGACGACCAGGACAGCTACGAATTTCTTTGTGATTTTATCAACACCGTT
TCCGGTAAGGGTGAGTGTGAGATGTTTATCATCCACGCCCGTAAAGCCTGGCTTTCCGGTTTAAGCCCGAAAGAAAACAG
GGAGATCCCGCCACTTGATTATCCTCGTGTCTATCAGCTGAAGCGCGATTTCCCCCATTTAACAATGTCGATTAACGGCG
GGATCAAGTCGCTGGAGGAAGCCAAAATGCATCTGCAGCATATGGATGGCGTAATGGTCGGGCGTGAGGCTTATCAGAAT
CCTGGTATTCTGGCGGCGGTAGATCGAGAAATTTTTGCTTCTACAGATACAGATGCTGATCCGGTCGCAGTGGTGCGGGC
AATGTACCCCTACATTGAACGTGAACTTAGCCAGGGAACGTATCTGGGGCATATCACGCGGCATATGCTGGGGCTGTTCC
AGGGGATTCCAGGAGCACGTCAATGGCGGCGTTATTTAAGCGAAAACGCCCATAAACCCGGCGCGGATATTAACGTACTG
GAACATGCTTTAAAGCTGGTTGCAGATAATCGTTAA

Upstream 100 bases:

>100_bases
AGATCCCCGGTTACTCAAGCCCTTCACCAGAGCATTGCCTTCTGGCTTAATCGTTATGCTATAGTAGCGCCCCCATTTAC
CAAATGATTAAAAAATCGCC

Downstream 100 bases:

>100_bases
TTTTTAACCAAAACTTAGTCAAATTCACCACGCCCTGCGTTAACTCGCGGGGCGTTTTGTTTATAAATCAATAGATTATT
TGTTGGCATGATTCTTGTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSAESQTAYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALA
QCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKMHLQHMDGVMVGREAYQN
PGILAAVDREIFASTDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKPGADINVL
EHALKLVADNR

Sequences:

>Translated_331_residues
MSAESQTAYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALA
QCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKMHLQHMDGVMVGREAYQN
PGILAAVDREIFASTDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKPGADINVL
EHALKLVADNR
>Mature_330_residues
SAESQTAYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQ
CAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVS
GKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKMHLQHMDGVMVGREAYQNP
GILAAVDREIFASTDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKPGADINVLE
HALKLVADNR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=299, Percent_Identity=24.0802675585284, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=98.1191222570533, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=246, Percent_Identity=26.4227642276423, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=25.7261410788382, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36957; Mature: 36825

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAESQTAYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAY
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCEEEECCCCEEEE
SEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCHHH
VADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCC
LSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKMHLQHMDGVMVGREAYQN
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCC
PGILAAVDREIFASTDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGAR
CCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
QWRRYLSENAHKPGADINVLEHALKLVADNR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAESQTAYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAY
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCEEEECCCCEEEE
SEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCHHH
VADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCC
LSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKMHLQHMDGVMVGREAYQN
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCC
PGILAAVDREIFASTDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGAR
CCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
QWRRYLSENAHKPGADINVLEHALKLVADNR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]