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The map label for this gene is rbsR [H]

Identifier: 218551287

GI number: 218551287

Start: 4159124

End: 4160116

Strand: Direct

Name: rbsR [H]

Synonym: EFER_4052

Alternate gene names: 218551287

Gene position: 4159124-4160116 (Clockwise)

Preceding gene: 218551286

Following gene: 218551290

Centisome position: 90.64

GC content: 48.84

Gene sequence:

>993_bases
TTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTCATCAATAAAGATCGCTT
CGTCAGTGAAGCGATTACTGCCAAAGTTGAAGCGGCAATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCC
TCAAGCTCAATCAGACTCGAACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTTTATTCTGAACTGGTGCGTGGC
GTTGAACGCAGCTGCTTCGAACGCGGCTATAGTCTTGTTCTTTGCAATACCGAAGGCGATGAGCAGCGGATGAATCGTAA
TCTGGAAACGCTGATGCAAAAGCGCGTTGATGGCTTGCTGTTACTGTGCACAGAAACACATCAGCCTTCGCGTGAAATCA
TGCAACGTTATCCGACAGTGCCTACCGTGATGATGGACTGGGCACCGTTCGATGGCGACAGCGATCTCATTCAGGATAAT
TCGTTGCTGGGCGGAGATTTAGCGACGCAACATCTTATTGATAATGGTTTTACGCGAATCGCCTGTATAACGGGGCCGCT
GGATAAAACACCAGCTCGATTGCGTCTGGAAGGGTATCGTACGGCAATGCAGCGTGCAGGCTTAACTATTCCTGCAGGCT
ATGAAATTACGGGGAATTTTGAGTTTGGTGGTGGTTTTGAAGCGATGCAAACGCTGCTTATGCATAAAGAAAGACCACAG
GCGGTATTTGCCGGAAACGATGCGATGGCTGTCGGCGCTTATCAGGCACTCTATCAGGCAGGGCTACAAATTCCTCGGGA
TATCGCCGTCGTCGGTTACGATGATATTGAACTTGCGCGTTATATGACTCCACCATTGACCACAATTCATCAACCAAAAG
ATGAACTGGGTGAACTGGCGATTGATGTGCTAATACATCGAATGGCGAAACCTGAAATGCAACAGCAGCGTCTACAACTG
ACACCCGTTTTAATGGCCCGTGGTTCGGTTTAA

Upstream 100 bases:

>100_bases
TCATGCCGCCGCAGCGATTGCTGTAACGCGCAAAGGCGCACAACCTTCCGTTCCATGGCGTGAAGAGATCGACGCATTTT
TAGACAGGCAGAGGTGACGC

Downstream 100 bases:

>100_bases
ATCAAACGGTATGGACAGATCCATATCCATACCGCTCTGTTTATTTATCCGGACGCTCGTTAGGAGCCGCAAGTAAATCC
TGACACGCTTTAAATGCGCG

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQHLIDNGFTRIACITGPLDKTPARLRLEGYRTAMQRAGLTIPAGYEITGNFEFGGGFEAMQTLLMHKERPQ
AVFAGNDAMAVGAYQALYQAGLQIPRDIAVVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAKPEMQQQRLQL
TPVLMARGSV

Sequences:

>Translated_330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQHLIDNGFTRIACITGPLDKTPARLRLEGYRTAMQRAGLTIPAGYEITGNFEFGGGFEAMQTLLMHKERPQ
AVFAGNDAMAVGAYQALYQAGLQIPRDIAVVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAKPEMQQQRLQL
TPVLMARGSV
>Mature_329_residues
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
LLGGDLATQHLIDNGFTRIACITGPLDKTPARLRLEGYRTAMQRAGLTIPAGYEITGNFEFGGGFEAMQTLLMHKERPQA
VFAGNDAMAVGAYQALYQAGLQIPRDIAVVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAKPEMQQQRLQLT
PVLMARGSV

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=90.273556231003, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=45.0151057401813, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=34.6278317152104, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.7883959044369, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.2258064516129, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=29.8192771084337, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=29.153605015674, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=28.3387622149837, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=28.7009063444109, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=26.1261261261261, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=26.4150943396226, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=25, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=25.5520504731861, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790192, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=23.4177215189873, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36554; Mature: 36422

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTR
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQHLIDNGFTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ACITGPLDKTPARLRLEGYRTAMQRAGLTIPAGYEITGNFEFGGGFEAMQTLLMHKERPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCC
AVFAGNDAMAVGAYQALYQAGLQIPRDIAVVGYDDIELARYMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRMAKPEMQQQRLQLTPVLMARGSV
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQHLIDNGFTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ACITGPLDKTPARLRLEGYRTAMQRAGLTIPAGYEITGNFEFGGGFEAMQTLLMHKERPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCC
AVFAGNDAMAVGAYQALYQAGLQIPRDIAVVGYDDIELARYMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRMAKPEMQQQRLQLTPVLMARGSV
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]