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The map label for this gene is gmuD [H]

Identifier: 218551193

GI number: 218551193

Start: 4060055

End: 4061473

Strand: Direct

Name: gmuD [H]

Synonym: EFER_3958

Alternate gene names: 218551193

Gene position: 4060055-4061473 (Clockwise)

Preceding gene: 218551192

Following gene: 218551194

Centisome position: 88.48

GC content: 47.0

Gene sequence:

>1419_bases
ATGAATCAAGGAGTAAGAACAATGAGATACCGTTTTCCCGACAACTTCTGGTGGGGTAGTGCTTGCTCTGCGCTGCAAAC
CGAAGGGGATAGTCTGAACGGCGGCAAAAGCCAAACCACCTGGGATGTATGGTTCGAGCGCCAGCCCGGCCGCTTCCATC
AAGGTGTTGGCCCGGCGAACACATCAACCTTTTATCAACACTGGCAACAAGATATTGCCCTGCTGAAACAGTTAAAACAT
AACAGTTTTCGCACCTCGTTAAGCTGGTCTCGCCTGATACCAGACGGTACTGGTGACGTAAATCCTGAAGCGGTGGCGTT
CTATAACAATGTCATCGACGAGCTGCTGGCGCAGGGCATCACACCGTTTATCACCTTGTTCCACTTTGATATGCCGATGG
TGATGCAGGAAAAAGGTGGCTGGGAGAGTCGTGAAGTCGTAGAAGCTTTTGGTGAATATGCCCGTACCTGTTTTGAACTG
TTCGGTAACAGAGTGAAGCACTGGTTTACCTTTAACGAACCGATTGTGCCGGTAGAGGGCGGTTATTTATATGACTTCCA
CTATCCGAACGTTGTTGATTTTAAACGTGCTGCGACAGTGGCTTACCATACAGTTCTTGCGCACGCCACGGCAGTTCGGG
CATTCCGCCGCGGTAATTTCGAAGGTGAAATTGGCGTGGTGCTCAACCTGACGCCTTCTTATCCACGCTCGCAAAATCCG
GCTGATGTTAAAGCCGCACACTGCGCTGACCTTCTGTTTAACCGCAGCTTCCTCGATCCGGTGCTAAAAGGGGAATACCC
GGCAGACCTTGTAGCATTACTGAAAGAGTACGATCAGTTGCCGCAATGCCAGCCTGACGATAGTCAGCTAATCGCCGACG
GAAAAATCGACCTGCTGGGGATAAATTATTATCAACCCCGACGCGTTAAATGCCGGGATAGCGCCGTAAACCCCAATGCC
CCCTTTATGCCGGAGTGGTTATTTGACTATTACGAGATGCCTGGGCGAAAAATGAATCCTTATCGTGGATGGGAAATTTA
TGAACCCGGTATTTACGATATCATCTCCAATCTTCGCGATAATTATGGTAATCCTCGTTGTTTTATTTCTGAAAATGGGA
TGGGTGTAGAAAACGAGCAACGTTTTATTCAGGAAGGGCAAGTCAACGATAGCTATCGTATTGAATTTGTCGCTGAACAT
TTAAAATGGCTGCATAAAGGAATTAGCGAAGGGTGTAATTGCCTTGGCTATCATATGTGGACGTTTATCGATAACTGGTC
ATGGCTTAATGCTTATAAAAATCGCTATGGATTTGTTCAGCTTGATCTGGAAACGCAAACACGTACGATTAAAAAAAGTG
GTGAGTGGTTTGCGACAGTTGCAGAAAATAACGGTTTTGATTGCGAGAAACAAAAATGA

Upstream 100 bases:

>100_bases
TCCCATTCTTGCGCGCCTATGAACGCTCTTTGCTGAAAAGCGAAGAACAAAAAGCGCAGGATCCCGTCTCCGTGGCCAAT
GCAGCCCGTAGCAACTCATA

Downstream 100 bases:

>100_bases
TCGAACTAGAAGATGCAGTTATGGAAATAATCGTCAATGCCGGCCAGTCACGCAGCCTCTGTTTTGAAGCGTTACACGCG
GCACGTGCCGGGAACTTTGA

Product: glycosyl phosphatase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNQGVRTMRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPGRFHQGVGPANTSTFYQHWQQDIALLKQLKH
NSFRTSLSWSRLIPDGTGDVNPEAVAFYNNVIDELLAQGITPFITLFHFDMPMVMQEKGGWESREVVEAFGEYARTCFEL
FGNRVKHWFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHATAVRAFRRGNFEGEIGVVLNLTPSYPRSQNP
ADVKAAHCADLLFNRSFLDPVLKGEYPADLVALLKEYDQLPQCQPDDSQLIADGKIDLLGINYYQPRRVKCRDSAVNPNA
PFMPEWLFDYYEMPGRKMNPYRGWEIYEPGIYDIISNLRDNYGNPRCFISENGMGVENEQRFIQEGQVNDSYRIEFVAEH
LKWLHKGISEGCNCLGYHMWTFIDNWSWLNAYKNRYGFVQLDLETQTRTIKKSGEWFATVAENNGFDCEKQK

Sequences:

>Translated_472_residues
MNQGVRTMRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPGRFHQGVGPANTSTFYQHWQQDIALLKQLKH
NSFRTSLSWSRLIPDGTGDVNPEAVAFYNNVIDELLAQGITPFITLFHFDMPMVMQEKGGWESREVVEAFGEYARTCFEL
FGNRVKHWFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHATAVRAFRRGNFEGEIGVVLNLTPSYPRSQNP
ADVKAAHCADLLFNRSFLDPVLKGEYPADLVALLKEYDQLPQCQPDDSQLIADGKIDLLGINYYQPRRVKCRDSAVNPNA
PFMPEWLFDYYEMPGRKMNPYRGWEIYEPGIYDIISNLRDNYGNPRCFISENGMGVENEQRFIQEGQVNDSYRIEFVAEH
LKWLHKGISEGCNCLGYHMWTFIDNWSWLNAYKNRYGFVQLDLETQTRTIKKSGEWFATVAENNGFDCEKQK
>Mature_472_residues
MNQGVRTMRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPGRFHQGVGPANTSTFYQHWQQDIALLKQLKH
NSFRTSLSWSRLIPDGTGDVNPEAVAFYNNVIDELLAQGITPFITLFHFDMPMVMQEKGGWESREVVEAFGEYARTCFEL
FGNRVKHWFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHATAVRAFRRGNFEGEIGVVLNLTPSYPRSQNP
ADVKAAHCADLLFNRSFLDPVLKGEYPADLVALLKEYDQLPQCQPDDSQLIADGKIDLLGINYYQPRRVKCRDSAVNPNA
PFMPEWLFDYYEMPGRKMNPYRGWEIYEPGIYDIISNLRDNYGNPRCFISENGMGVENEQRFIQEGQVNDSYRIEFVAEH
LKWLHKGISEGCNCLGYHMWTFIDNWSWLNAYKNRYGFVQLDLETQTRTIKKSGEWFATVAENNGFDCEKQK

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=490, Percent_Identity=30.8163265306122, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI13273313, Length=480, Percent_Identity=29.7916666666667, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI32481206, Length=491, Percent_Identity=30.5498981670061, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI28376633, Length=474, Percent_Identity=24.4725738396624, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI24497614, Length=479, Percent_Identity=25.678496868476, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789070, Length=492, Percent_Identity=33.739837398374, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI2367174, Length=497, Percent_Identity=31.1871227364185, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI2367270, Length=478, Percent_Identity=29.4979079497908, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17539390, Length=473, Percent_Identity=31.9238900634249, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17552856, Length=471, Percent_Identity=30.9978768577495, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI21356577, Length=481, Percent_Identity=27.8586278586279, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54593; Mature: 54593

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQGVRTMRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPGRFHQGVGPAN
CCCCCCHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHCCCCCCC
TSTFYQHWQQDIALLKQLKHNSFRTSLSWSRLIPDGTGDVNPEAVAFYNNVIDELLAQGI
HHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
TPFITLFHFDMPMVMQEKGGWESREVVEAFGEYARTCFELFGNRVKHWFTFNEPIVPVEG
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCC
GYLYDFHYPNVVDFKRAATVAYHTVLAHATAVRAFRRGNFEGEIGVVLNLTPSYPRSQNP
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
ADVKAAHCADLLFNRSFLDPVLKGEYPADLVALLKEYDQLPQCQPDDSQLIADGKIDLLG
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEE
INYYQPRRVKCRDSAVNPNAPFMPEWLFDYYEMPGRKMNPYRGWEIYEPGIYDIISNLRD
ECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHH
NYGNPRCFISENGMGVENEQRFIQEGQVNDSYRIEFVAEHLKWLHKGISEGCNCLGYHMW
CCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFIDNWSWLNAYKNRYGFVQLDLETQTRTIKKSGEWFATVAENNGFDCEKQK
HHHCCCHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNQGVRTMRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPGRFHQGVGPAN
CCCCCCHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHCCCCCCC
TSTFYQHWQQDIALLKQLKHNSFRTSLSWSRLIPDGTGDVNPEAVAFYNNVIDELLAQGI
HHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
TPFITLFHFDMPMVMQEKGGWESREVVEAFGEYARTCFELFGNRVKHWFTFNEPIVPVEG
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCC
GYLYDFHYPNVVDFKRAATVAYHTVLAHATAVRAFRRGNFEGEIGVVLNLTPSYPRSQNP
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
ADVKAAHCADLLFNRSFLDPVLKGEYPADLVALLKEYDQLPQCQPDDSQLIADGKIDLLG
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEE
INYYQPRRVKCRDSAVNPNAPFMPEWLFDYYEMPGRKMNPYRGWEIYEPGIYDIISNLRD
ECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHH
NYGNPRCFISENGMGVENEQRFIQEGQVNDSYRIEFVAEHLKWLHKGISEGCNCLGYHMW
CCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFIDNWSWLNAYKNRYGFVQLDLETQTRTIKKSGEWFATVAENNGFDCEKQK
HHHCCCHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]