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The map label for this gene is malS [H]

Identifier: 218550853

GI number: 218550853

Start: 3652839

End: 3654947

Strand: Direct

Name: malS [H]

Synonym: EFER_3569

Alternate gene names: 218550853

Gene position: 3652839-3654947 (Clockwise)

Preceding gene: 218550851

Following gene: 218550854

Centisome position: 79.6

GC content: 50.5

Gene sequence:

>2109_bases
ATGATTACCAGGGAGGAGTTTTACCGGACTTATCACTGGCAAACTGGTGAAATTCGCAGCAATAAGAGCTCATCCATTAT
GAAACTTGCCGCCTGTTTTCTGCTACTTCTGCCTGGCGTTGCCGTTGCCGCCACCTGGACTTCCGGGGGATTTCCCACCT
TTGACGAACAAAGTACAGGTAAATATGCCAGTCACGCGCAGCTGACCAAAGGTACGCGCGCGTTAACGTTAAATTTTGAC
CAACAGTGCTGGCAACCTGCGGATGCCATAAAACTCAATCAGATGCTTTCCCTGAAACCTTGTGACAGCACGCCTCCCCA
GTGGCGATTATTCCGCGATGGCGAGTACACCTTACAAGTTGATACACGCTCTGGCACGCCAACGTTGATGATTTCGGTGA
CCAGCACCGCACAACCAACAATAAATCTGATACGTGAATGCCCGAAATGGGATGGGCAACCGCTCACTCTGGATGTCAGT
CAAACCTTTCCTGAAGGCGCTGCGATACGTGAATACTACAGTCAGCAAATCGTTACGGTGAAAAATGGCCATATCACCTT
AAAACCGGTAACTGAAAGTAACGGCTTACTGCTTCTGGAACACGCAGAAACGCATACTTCCGCCCCTTTCGACTGGCATA
ACGCCACAGTTTACTTTGTACTGACAGACCGTTTTGAAAATGGTGATCCCGGCAATGACCAAAGTTACGGACGCCATAAA
GATGGCATGCAGGAAATTGGCACCTTCCACGGTGGTGATTTACGCGGGCTGACCAACAAACTGGATTATCTCCAGCAACT
GGGCGTCAATGCTTTATGGATCAGCGCCCCGTTTGAGCAAATTCATGGCTGGGTTGGCGGCGGTACAAAAGGCGACTTCC
CGCATTATGCCTATCATGGCTATTACACCCAGGACTGGACGAACCTTGATGCCAATATAGGCAGCGAAGCCGAACTGCGG
GCACTGGTTGACGGTGCGCACCAGCGCGGTATCCGAATTATTTTTGATGTGGTGATGAACCATACTGGCTATGCCACGCT
GGCGGATATGCAGGAGTTTCAGTTCGGCGCGTTATACCTTTCCGGTGACGAGCTGAAAAAAACACTGGGCGAGCGCTGGA
GTGACTGGAAGCCCGCTGCCGGGCAAACATGGCATAGCTTTAACGATTACATCAATTTCAGCGACAAAACAGGCTGGGAA
AAGTGGTGGGGAAAAAACTGGATCCGTACCGATATTGGTGATTACGACAATCCCGGTTTTGACGATCTCACCATGTCGCT
GGCCTTTCTACCGGATATAAAAACTGAATCCACTACCGCTTCTGGCCTGCCGGTATTCTATAAAAACAAACCGGATACTC
ACGCCAAAGCCATCGATGGTTATACGCCGCGCGATTATTTAACTCACTGGCTAAGCCAATGGGTACGCGATTATGGTATC
GACGGATTCCGGGTGGATACGGCCAAACATGTTGAGTTGCCCGCCTGGCAACAACTAAAAACCGAAGCCAGTGCCGCACT
TAACGAGTGGAAGAAAGCTAATCCCGAAAAAGCGTTAGATGACAATTCCTTCTGGATGACCGGAGAAGCCTGGGGTCATG
GCGTGATGCAAAGCGACTACTACCGCAACGGCTTCGACGCGATGATCAATTTCGATTATCAGGAGCAAGCAGCGAAAGCG
GTAGATTGCCTGGCACAGATGGACAACATCTGGCAGCAAATGGCAGAGAAATTGCAGGATTTCAACGTACTAAGTTATCT
CTCCTCACACGATACTCGTCTGTTCCGCGAAGGTGGCAAAAAATCAGCAGAACTGCTTTTACTCGCTCCTGGTGCAGTGC
AAATTTTTTACGGTGATGAATCCTTACGTCCGTTTGGCCCCACCGGTTCAGATCCGTTGCAAGGCACTCGTTCGGATATG
AACTGGCAGGATGTCAGCGATAAATCCGCCGCCAGCGTCAAGCACTGGCAGCGCATCAGCCAGTTCCGCGCCCGTCACCT
GGCAATAGGCGCAGGCAAACAAACCACGTTATCTTTGCCACAAGGTTACGGATTTGTTCGCGAACATGGCGACGATAAAG
TGATGGTTATCTGGGCGGGCCAACATTAA

Upstream 100 bases:

>100_bases
ATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGTTTATCGCAAAAAATACGCTTAAATCCCGGGCCAAATACTCACCC
TTTCGCCTCATCCTCGTGCA

Downstream 100 bases:

>100_bases
TGGTTTTGGCTTCCCGTGTGGCAAACTGCCGGGAAGCCTTATCAGAATCAAATTCTATTTAATAGACAGTACCCAGCATA
AACCGCCAATCACGCCACTT

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MITREEFYRTYHWQTGEIRSNKSSSIMKLAACFLLLLPGVAVAATWTSGGFPTFDEQSTGKYASHAQLTKGTRALTLNFD
QQCWQPADAIKLNQMLSLKPCDSTPPQWRLFRDGEYTLQVDTRSGTPTLMISVTSTAQPTINLIRECPKWDGQPLTLDVS
QTFPEGAAIREYYSQQIVTVKNGHITLKPVTESNGLLLLEHAETHTSAPFDWHNATVYFVLTDRFENGDPGNDQSYGRHK
DGMQEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANIGSEAELR
ALVDGAHQRGIRIIFDVVMNHTGYATLADMQEFQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWE
KWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKPDTHAKAIDGYTPRDYLTHWLSQWVRDYGI
DGFRVDTAKHVELPAWQQLKTEASAALNEWKKANPEKALDDNSFWMTGEAWGHGVMQSDYYRNGFDAMINFDYQEQAAKA
VDCLAQMDNIWQQMAEKLQDFNVLSYLSSHDTRLFREGGKKSAELLLLAPGAVQIFYGDESLRPFGPTGSDPLQGTRSDM
NWQDVSDKSAASVKHWQRISQFRARHLAIGAGKQTTLSLPQGYGFVREHGDDKVMVIWAGQH

Sequences:

>Translated_702_residues
MITREEFYRTYHWQTGEIRSNKSSSIMKLAACFLLLLPGVAVAATWTSGGFPTFDEQSTGKYASHAQLTKGTRALTLNFD
QQCWQPADAIKLNQMLSLKPCDSTPPQWRLFRDGEYTLQVDTRSGTPTLMISVTSTAQPTINLIRECPKWDGQPLTLDVS
QTFPEGAAIREYYSQQIVTVKNGHITLKPVTESNGLLLLEHAETHTSAPFDWHNATVYFVLTDRFENGDPGNDQSYGRHK
DGMQEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANIGSEAELR
ALVDGAHQRGIRIIFDVVMNHTGYATLADMQEFQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWE
KWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKPDTHAKAIDGYTPRDYLTHWLSQWVRDYGI
DGFRVDTAKHVELPAWQQLKTEASAALNEWKKANPEKALDDNSFWMTGEAWGHGVMQSDYYRNGFDAMINFDYQEQAAKA
VDCLAQMDNIWQQMAEKLQDFNVLSYLSSHDTRLFREGGKKSAELLLLAPGAVQIFYGDESLRPFGPTGSDPLQGTRSDM
NWQDVSDKSAASVKHWQRISQFRARHLAIGAGKQTTLSLPQGYGFVREHGDDKVMVIWAGQH
>Mature_702_residues
MITREEFYRTYHWQTGEIRSNKSSSIMKLAACFLLLLPGVAVAATWTSGGFPTFDEQSTGKYASHAQLTKGTRALTLNFD
QQCWQPADAIKLNQMLSLKPCDSTPPQWRLFRDGEYTLQVDTRSGTPTLMISVTSTAQPTINLIRECPKWDGQPLTLDVS
QTFPEGAAIREYYSQQIVTVKNGHITLKPVTESNGLLLLEHAETHTSAPFDWHNATVYFVLTDRFENGDPGNDQSYGRHK
DGMQEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANIGSEAELR
ALVDGAHQRGIRIIFDVVMNHTGYATLADMQEFQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWE
KWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKPDTHAKAIDGYTPRDYLTHWLSQWVRDYGI
DGFRVDTAKHVELPAWQQLKTEASAALNEWKKANPEKALDDNSFWMTGEAWGHGVMQSDYYRNGFDAMINFDYQEQAAKA
VDCLAQMDNIWQQMAEKLQDFNVLSYLSSHDTRLFREGGKKSAELLLLAPGAVQIFYGDESLRPFGPTGSDPLQGTRSDM
NWQDVSDKSAASVKHWQRISQFRARHLAIGAGKQTTLSLPQGYGFVREHGDDKVMVIWAGQH

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=89.2011834319527, Blast_Score=1273, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=143, Percent_Identity=30.0699300699301, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 79300; Mature: 79300

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITREEFYRTYHWQTGEIRSNKSSSIMKLAACFLLLLPGVAVAATWTSGGFPTFDEQSTG
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
KYASHAQLTKGTRALTLNFDQQCWQPADAIKLNQMLSLKPCDSTPPQWRLFRDGEYTLQV
CCHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEE
DTRSGTPTLMISVTSTAQPTINLIRECPKWDGQPLTLDVSQTFPEGAAIREYYSQQIVTV
ECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHCCCCCCHHHHHHHCCEEEEE
KNGHITLKPVTESNGLLLLEHAETHTSAPFDWHNATVYFVLTDRFENGDPGNDQSYGRHK
ECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
DGMQEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHG
CCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECC
YYTQDWTNLDANIGSEAELRALVDGAHQRGIRIIFDVVMNHTGYATLADMQEFQFGALYL
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEHHHHCCCCCEEHHHHHHCCEEEEEE
SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWEKWWGKNWIRTDIGDYDNPGF
CHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHCCCCEECCCCCCCCCCC
DDLTMSLAFLPDIKTESTTASGLPVFYKNKPDTHAKAIDGYTPRDYLTHWLSQWVRDYGI
HHHHEEEEECCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC
DGFRVDTAKHVELPAWQQLKTEASAALNEWKKANPEKALDDNSFWMTGEAWGHGVMQSDY
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHCCCCCCCCC
YRNGFDAMINFDYQEQAAKAVDCLAQMDNIWQQMAEKLQDFNVLSYLSSHDTRLFREGGK
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
KSAELLLLAPGAVQIFYGDESLRPFGPTGSDPLQGTRSDMNWQDVSDKSAASVKHWQRIS
CCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QFRARHLAIGAGKQTTLSLPQGYGFVREHGDDKVMVIWAGQH
HHHHHHEEECCCCCEEEECCCCCCHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MITREEFYRTYHWQTGEIRSNKSSSIMKLAACFLLLLPGVAVAATWTSGGFPTFDEQSTG
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
KYASHAQLTKGTRALTLNFDQQCWQPADAIKLNQMLSLKPCDSTPPQWRLFRDGEYTLQV
CCHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEE
DTRSGTPTLMISVTSTAQPTINLIRECPKWDGQPLTLDVSQTFPEGAAIREYYSQQIVTV
ECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHCCCCCCHHHHHHHCCEEEEE
KNGHITLKPVTESNGLLLLEHAETHTSAPFDWHNATVYFVLTDRFENGDPGNDQSYGRHK
ECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
DGMQEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHG
CCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECC
YYTQDWTNLDANIGSEAELRALVDGAHQRGIRIIFDVVMNHTGYATLADMQEFQFGALYL
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEHHHHCCCCCEEHHHHHHCCEEEEEE
SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWEKWWGKNWIRTDIGDYDNPGF
CHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHCCCCEECCCCCCCCCCC
DDLTMSLAFLPDIKTESTTASGLPVFYKNKPDTHAKAIDGYTPRDYLTHWLSQWVRDYGI
HHHHEEEEECCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC
DGFRVDTAKHVELPAWQQLKTEASAALNEWKKANPEKALDDNSFWMTGEAWGHGVMQSDY
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHCCCCCCCCC
YRNGFDAMINFDYQEQAAKAVDCLAQMDNIWQQMAEKLQDFNVLSYLSSHDTRLFREGGK
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
KSAELLLLAPGAVQIFYGDESLRPFGPTGSDPLQGTRSDMNWQDVSDKSAASVKHWQRIS
CCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QFRARHLAIGAGKQTTLSLPQGYGFVREHGDDKVMVIWAGQH
HHHHHHEEECCCCCEEEECCCCCCHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]