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The map label for this gene is treF [H]

Identifier: 218550791

GI number: 218550791

Start: 3577746

End: 3579395

Strand: Direct

Name: treF [H]

Synonym: EFER_3502

Alternate gene names: 218550791

Gene position: 3577746-3579395 (Clockwise)

Preceding gene: 218550790

Following gene: 218550793

Centisome position: 77.97

GC content: 50.55

Gene sequence:

>1650_bases
ATGCTCAATCAGAAAATCCAAAATCCTAATCCAGACGAACTGATGATCGAGATCGATCTCTGCTATGAACTGGATCCGTA
TGAGTTAAAACTGGATGAGATGATAGAAGCAGAACCGGAGCCAGAGATGATTGAGGGGCTTCCTGCCTCCGATGCTCTGA
CACCTGCCGACCGTTATCTCGAACTGTTTGAGCACGTGCAGTCGGCGAAAATTTTTCCCGACAGCAAAACGTTTCCTGAC
TGCGCACCGAAAATGGACCCGCTCGATATTTTAATTCGCTATCGCAAAGTCCGTCGTCACCGGGATTTCGATTTACGCCA
GTTTGTGGAAAATCACTTCTGGCTACCGGAAGTCTATTCCAGCGAGTATGTCTCTAACCCGGAGAACTCCCTGAAAGAGC
ACATTGACCAGCTATGGCCTGTACTCACCCGCGAACCACAGGATCATATTCCCTGGTCATCACTGTTGGCGCTACCGCAA
TCCTATATCGTTCCCGGCGGACGATTTAGCGAAACGTACTACTGGGATTCCTACTTCACCATGCTGGGGCTGGCGGAAAG
TGGCCGCGAAGATTTGCTGAAATGCATGGCTGATAACTTTGCCTGGATGATCGAAAACTATGGTCATATCCCTAATGGCA
ACCGTACCTACTATTTAAGCCGCTCGCAGCCACCCGTTTTTGCTTTGATGGTGGAACTGTTTGAAGAAGATGGCGTACGC
GGCGCACGCCGTTACCTGGACCATCTGAAAATGGAATATGCCTTTTGGATGGATGGCGCAGAGTCACTTATCCCTAATCA
AGCCTATCGTCATGTGGTAAGAATGCCTGACGGCTCGCTGCTCAATCGTTACTGGGATGACCGCGATACCCCACGCGATG
AGTCGTGGCTGGAGGATGTGGAAACGGCAAAACATTCTGGTCGCCCTCCTAATGAGGTGTATCGTGATTTGCGTGCAGGC
GCAGCTTCCGGCTGGGATTACTCCTCCCGTTGGCTACGTGATGCCGGACGGCTGGCAAGTATTCGCACAACACAGTTTAT
TCCCATCGATCTCAACGCGTTTCTGTTCAAACTGGAGAGTGCTATCGCCAATATCTCAGCGCTAAAAGGCGATAAGGAGA
CGGAAACACTGTTTCGCCAGAAAGCCAACGCCCGCCGCGATGCGGTAAACCGTTATCTCTGGGATGATGAAAATGGTATT
TACCGTGATTACGACTGGCGACGCGAGCAGTTAGCTCTGTTTTCTGCCGCTGCAATTGTGCCGCTGTATGTTGGTATGGC
GAGCCATGAACAGGCAGATCGGTTGGGCGATGCTGTCCGCAACCGTCTTTTAACACCAGGCGGCATATTAGCGACAGAGT
ATGAAACAGGCGAACAGTGGGATAAACCCAACGGCTGGGCACCGCTGCAATGGATGGCGATTCAGGGCTTTAAAATGTAT
GGTGATGACCATCTTGGCGATGAAATTGCCCATAGCTGGTTACAAACTGTAAACCTGTTTTATCAACAGCATCATAAGCT
TATCGAGAAGTATCACATTGCGGGCGGTACACCGCGTGAAGGGGGCGGTGGTGAATATCCATTACAGGATGGTTTTGGCT
GGACAAACGGTGTTGTCCGGCGGTTAATCAGCCTCTATGGTGAACCTTAA

Upstream 100 bases:

>100_bases
CGTGATCCACCGCACGCTTTGTCCCCCCACCATCGGAGCGAATGACTACCCTTAAAGGAAAACCCGATTTTAAGTGATCA
ATTTCGGAGGTCGGATCCTT

Downstream 100 bases:

>100_bases
AGCCAGGCAGTACCCGGCTTTAAGCCCAGGATTCAGGAAAGGTGATTTAACATCATCGCCGCCTGGGTACGATTTTTAAC
ATCCAGCCGTCGGTACAGTG

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRQFVENHFWLPEVYSSEYVSNPENSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGDKETETLFRQKANARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMASHEQADRLGDAVRNRLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDHLGDEIAHSWLQTVNLFYQQHHKLIEKYHIAGGTPREGGGGEYPLQDGFGWTNGVVRRLISLYGEP

Sequences:

>Translated_549_residues
MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRQFVENHFWLPEVYSSEYVSNPENSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGDKETETLFRQKANARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMASHEQADRLGDAVRNRLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDHLGDEIAHSWLQTVNLFYQQHHKLIEKYHIAGGTPREGGGGEYPLQDGFGWTNGVVRRLISLYGEP
>Mature_549_residues
MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRQFVENHFWLPEVYSSEYVSNPENSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGDKETETLFRQKANARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMASHEQADRLGDAVRNRLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDHLGDEIAHSWLQTVNLFYQQHHKLIEKYHIAGGTPREGGGGEYPLQDGFGWTNGVVRRLISLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=525, Percent_Identity=34.4761904761905, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=95.8105646630237, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=51.5400410677618, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.8785578747628, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI25141398, Length=527, Percent_Identity=32.0683111954459, Blast_Score=238, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17565078, Length=516, Percent_Identity=31.5891472868217, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI25148109, Length=549, Percent_Identity=29.8724954462659, Blast_Score=223, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71987755, Length=570, Percent_Identity=27.8947368421053, Blast_Score=192, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=479, Percent_Identity=29.0187891440501, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319473, Length=472, Percent_Identity=29.0254237288136, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24656680, Length=550, Percent_Identity=31.4545454545455, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656675, Length=550, Percent_Identity=31.4545454545455, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656661, Length=535, Percent_Identity=31.588785046729, Blast_Score=232, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17933716, Length=535, Percent_Identity=31.588785046729, Blast_Score=232, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24656670, Length=535, Percent_Identity=31.588785046729, Blast_Score=232, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24656685, Length=486, Percent_Identity=32.0987654320988, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI22024178, Length=538, Percent_Identity=26.2081784386617, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45551104, Length=380, Percent_Identity=26.0526315789474, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28573474, Length=295, Percent_Identity=26.7796610169492, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63712; Mature: 63712

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRQFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSNPENSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCEEEEECHHHHHHHHHH
AIANISALKGDKETETLFRQKANARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMASHEQADRLGDAVRNRLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHEECCCCCC
GDDHLGDEIAHSWLQTVNLFYQQHHKLIEKYHIAGGTPREGGGGEYPLQDGFGWTNGVVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLISLYGEP
HHHHHHCCC
>Mature Secondary Structure
MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRQFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSNPENSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCEEEEECHHHHHHHHHH
AIANISALKGDKETETLFRQKANARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMASHEQADRLGDAVRNRLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHEECCCCCC
GDDHLGDEIAHSWLQTVNLFYQQHHKLIEKYHIAGGTPREGGGGEYPLQDGFGWTNGVVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLISLYGEP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA