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The map label for this gene is pitA [H]

Identifier: 218550775

GI number: 218550775

Start: 3563293

End: 3564792

Strand: Direct

Name: pitA [H]

Synonym: EFER_3486

Alternate gene names: 218550775

Gene position: 3563293-3564792 (Clockwise)

Preceding gene: 218550773

Following gene: 218550777

Centisome position: 77.65

GC content: 51.2

Gene sequence:

>1500_bases
ATGCTACATTTGTTTACTGGTCTGGATTTACATACCGGGCTTTTATTATTGCTTGCTCTGGCCTTTGTGCTGTTCTATGA
AGCTATCAACGGCTTCCATGACACAGCCAATGCGGTGGCTACCGTTATCTACACCCGCGCGATGCGTTCGCAGCTGGCAG
TAGTAATGGCGGCAGTCTTCAACTTCCTGGGTGTTCTGCTTGGGGGTCTCAGTGTGGCCTATGCCATTGTGCATATGCTG
CCAACAGATTTGTTGCTCAATATGGGTTCCTCACATGGTCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTG
GAACCTCGGAACCTGGTACTTTGGTTTACCAGCATCCAGCTCCCATACACTGATTGGCGCAATTATCGGCATTGGTTTAA
CCAATGCGTTAATGACCGGCACCTCCGTTGTGGATGCACTCAACATCCCGAAAGTAATGAGCATTTTTGGTTCGCTCGTT
TTCTCTCCTATTGTTGGTCTGGTGTTTGCTGGTGGCCTGATCTTCTTACTACGTCGCTACTGGAGCGGCACCAAGAAGCG
TGCGCGTATCCACCTGACACCAGCAGAACGTGAAAAGAAAGACGGTAAGAAAAAACCGCCATTCTGGACGCGTATCGCGC
TGATCCTTTCCGCTATTGGCGTAGCGTTCTCCCACGGAGCGAACGATGGTCAGAAAGGTATTGGTCTGGTTATGCTGGTA
CTGATTGGCGTTGCTCCGGCAGGCTTCGTGGTGAACATGAATGCCTCTGGCTACGAAATCACCCGTACTCGTGATGCCAT
CAATAACGTAGAAGCTTACTTTGAGCAGAATTCTGCGTTGCTGAAACAAGTGACAGGAACTGAGCAAATCGTACCTATTC
CGCAAACGGGCACTGAACAACCTGAAGAGTTCCATTGTCATCCGGCTAATACCATCAACGCGCTGACTCGCCTGAAAGGC
ATGTTGACTACGGATGTGGAAAGCTACGACAAACTGACGCTTGATCAACGTAGTCAGATGCGCCGCATTATGCTGTGCGT
GTCCGATACCATTGATAAAGTGGTGAAAATGCCGGGCGTAAGCCCAGACGATCAACGACTGCTGAAGAAACTGAAGGGCG
ATATGCTGAGCACCATCGAGTATGCGCCGATTTGGATCATCATGGCGGTCGCGCTGGCGCTGGGTATTGGTACAATGATC
GGCTGGCGTCGCGTGGCAACGACCATCGGCGAGAAAATCGGTAAGAAAGGCATGACCTATGCTCAGGGGATGGCAGCACA
GATGACAGCTGCAGTTTCCATCGGTCTTGCCAGTTACACCGGGATGCCGGTTTCCACAACCCACGTACTCTCCTCCTCGG
TGGCAGGTACGATGGTCGTTGATGGCGGTGGTCTGCAACGTAAAACGGTGACCAGTATTCTGATGGCCTGGGTATTTACC
CTGCCAGCAGCAATTCTGCTTTCCGGTGGTCTGTACTGGATTTCCCTGCAGTTTATCTAA

Upstream 100 bases:

>100_bases
GAGGAGACAAATCAAAAAAAGTCCAGATTTCACTTTGCCCGCGCCGCCAAAGTCCCTGATAATGCGCCGCGTTCATGTCC
TCAAAATGGCGTAACGTCCT

Downstream 100 bases:

>100_bases
CAGGCAGTATTGAAATGAGCGGGTCAGGAAACTGGCCCGCTTTTTTTATCCTTAGTGCCAAATCAGCAATGCAATCAAAC
TAATCACCACCAGCCCACAA

Product: phosphate transporter, low-affinity

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLHLFTGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHML
PTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVMSIFGSLV
FSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAINNVEAYFEQNSALLKQVTGTEQIVPIPQTGTEQPEEFHCHPANTINALTRLKG
MLTTDVESYDKLTLDQRSQMRRIMLCVSDTIDKVVKMPGVSPDDQRLLKKLKGDMLSTIEYAPIWIIMAVALALGIGTMI
GWRRVATTIGEKIGKKGMTYAQGMAAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAILLSGGLYWISLQFI

Sequences:

>Translated_499_residues
MLHLFTGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHML
PTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVMSIFGSLV
FSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAINNVEAYFEQNSALLKQVTGTEQIVPIPQTGTEQPEEFHCHPANTINALTRLKG
MLTTDVESYDKLTLDQRSQMRRIMLCVSDTIDKVVKMPGVSPDDQRLLKKLKGDMLSTIEYAPIWIIMAVALALGIGTMI
GWRRVATTIGEKIGKKGMTYAQGMAAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAILLSGGLYWISLQFI
>Mature_499_residues
MLHLFTGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHML
PTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVMSIFGSLV
FSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAINNVEAYFEQNSALLKQVTGTEQIVPIPQTGTEQPEEFHCHPANTINALTRLKG
MLTTDVESYDKLTLDQRSQMRRIMLCVSDTIDKVVKMPGVSPDDQRLLKKLKGDMLSTIEYAPIWIIMAVALALGIGTMI
GWRRVATTIGEKIGKKGMTYAQGMAAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAILLSGGLYWISLQFI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=94.5891783567134, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=80.561122244489, Blast_Score=780, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53677; Mature: 53677

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFTGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVF
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNALMTGTSVVDALNIPKVMSIFGSLVFSPIVGLVFAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYEITRTRDAINNVEAYFEQNSALLKQVTGTEQIVPIPQTGTEQ
HHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
PEEFHCHPANTINALTRLKGMLTTDVESYDKLTLDQRSQMRRIMLCVSDTIDKVVKMPGV
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPDDQRLLKKLKGDMLSTIEYAPIWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AQGMAAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFT
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
LPAAILLSGGLYWISLQFI
HHHHHHHCCCEEEEEEEEC
>Mature Secondary Structure
MLHLFTGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVF
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNALMTGTSVVDALNIPKVMSIFGSLVFSPIVGLVFAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYEITRTRDAINNVEAYFEQNSALLKQVTGTEQIVPIPQTGTEQ
HHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
PEEFHCHPANTINALTRLKGMLTTDVESYDKLTLDQRSQMRRIMLCVSDTIDKVVKMPGV
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPDDQRLLKKLKGDMLSTIEYAPIWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AQGMAAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFT
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
LPAAILLSGGLYWISLQFI
HHHHHHHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]