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The map label for this gene is acpT [H]

Identifier: 218550756

GI number: 218550756

Start: 3532118

End: 3532702

Strand: Direct

Name: acpT [H]

Synonym: EFER_3467

Alternate gene names: 218550756

Gene position: 3532118-3532702 (Clockwise)

Preceding gene: 218550755

Following gene: 218550757

Centisome position: 76.97

GC content: 54.7

Gene sequence:

>585_bases
ATGTATCGAATCGTTCTGGGAAAAGTATCGACCTTAAGCGCAGCGGCACTGCCATCCGCGCTTATCGAGCAGGCACCGCA
GGGTGCCCGCCGTGCACGCTGGCTTGCTGGCCGTGCACTGCTTTCCCATGCGCTTTCGCCACTGCCAGAAATTGTCTATG
GCGAACAGGGCAAACCCGCCTTTTCCCCGGAGACTGCACTCTGGTTTAACCTCAGTCATAGCGGCGACGATATCGTGTTG
TTGTTAAGCGATGAAGGCGAAGTCGGTTGCGATATCGAAGTCATCCGTCCGCGTGCACAATGGCGTTCGCTGGCGAATGC
GGTGTTCAGCCAGGGCGAACATGAGGAACTGGAAGCAGAACATCCCGATCACCAACTCATGGCGTTCTGGCGTATCTGGA
CGCGAAAAGAAGCCATCGTTAAACAGCGTGGCGGCAGCGCCTGGCAAATTGTCAGCATCGACAGTACACAACAATCGGCG
CTCTCAGTCAGCCATTGTCAGTTTGGTTCATTGATCCTCGCAGTCTGCACGCCCACACCATTTGCTCTTACTCATAACGT
TCTACAGCAGGTAGAATCACTGTAA

Upstream 100 bases:

>100_bases
ATGGGTGAAGCCCGCAAGATTGATTGTGAGTTTTTGCAGAGTAACAACTTTGCCTTTGGTGGCATTAATACCTCCATCAT
CATAAAACGTTGGCCCTGAT

Downstream 100 bases:

>100_bases
TTCCTCTCTGACTGACCTGAACAATAAGGTGTAACTCAGATGCAACGTGTCACCATTACTCTCGATGACGATTTACTGGA
AACGCTGGATAACCTGAGTC

Product: holo-(acyl carrier protein) synthase 2

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MYRIVLGKVSTLSAAALPSALIEQAPQGARRARWLAGRALLSHALSPLPEIVYGEQGKPAFSPETALWFNLSHSGDDIVL
LLSDEGEVGCDIEVIRPRAQWRSLANAVFSQGEHEELEAEHPDHQLMAFWRIWTRKEAIVKQRGGSAWQIVSIDSTQQSA
LSVSHCQFGSLILAVCTPTPFALTHNVLQQVESL

Sequences:

>Translated_194_residues
MYRIVLGKVSTLSAAALPSALIEQAPQGARRARWLAGRALLSHALSPLPEIVYGEQGKPAFSPETALWFNLSHSGDDIVL
LLSDEGEVGCDIEVIRPRAQWRSLANAVFSQGEHEELEAEHPDHQLMAFWRIWTRKEAIVKQRGGSAWQIVSIDSTQQSA
LSVSHCQFGSLILAVCTPTPFALTHNVLQQVESL
>Mature_194_residues
MYRIVLGKVSTLSAAALPSALIEQAPQGARRARWLAGRALLSHALSPLPEIVYGEQGKPAFSPETALWFNLSHSGDDIVL
LLSDEGEVGCDIEVIRPRAQWRSLANAVFSQGEHEELEAEHPDHQLMAFWRIWTRKEAIVKQRGGSAWQIVSIDSTQQSA
LSVSHCQFGSLILAVCTPTPFALTHNVLQQVESL

Specific function: May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Escherichia coli, GI1789886, Length=194, Percent_Identity=79.3814432989691, Blast_Score=319, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 21331; Mature: 21331

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIVLGKVSTLSAAALPSALIEQAPQGARRARWLAGRALLSHALSPLPEIVYGEQGKPA
CEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
FSPETALWFNLSHSGDDIVLLLSDEGEVGCDIEVIRPRAQWRSLANAVFSQGEHEELEAE
CCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCC
HPDHQLMAFWRIWTRKEAIVKQRGGSAWQIVSIDSTQQSALSVSHCQFGSLILAVCTPTP
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCHHHEEEEEECCCC
FALTHNVLQQVESL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MYRIVLGKVSTLSAAALPSALIEQAPQGARRARWLAGRALLSHALSPLPEIVYGEQGKPA
CEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
FSPETALWFNLSHSGDDIVLLLSDEGEVGCDIEVIRPRAQWRSLANAVFSQGEHEELEAE
CCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCC
HPDHQLMAFWRIWTRKEAIVKQRGGSAWQIVSIDSTQQSALSVSHCQFGSLILAVCTPTP
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCHHHEEEEEECCCC
FALTHNVLQQVESL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]