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The map label for this gene is rtcR [H]

Identifier: 218550679

GI number: 218550679

Start: 3453455

End: 3455053

Strand: Direct

Name: rtcR [H]

Synonym: EFER_3390

Alternate gene names: 218550679

Gene position: 3453455-3455053 (Clockwise)

Preceding gene: 218550676

Following gene: 218550683

Centisome position: 75.26

GC content: 54.72

Gene sequence:

>1599_bases
ATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGCACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGATGGTCGAAATG
GCGTCCGTCACTCTGTCTATGCCAGCAAGAATCGTTAGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCACGCT
CGCTGTTTGAAACGCTTAAACGCGATATCGCCAATGTTTCGCCAGAAACAGAAGTGGTGGGCGTTGAGATTGAACTGCAT
AACCCATGGGATTTCGAGGAGGTTTACGCCTGCCTGCATGATTTCGCCCGTGGTTACGCGTTCGAACCTGATAAAGAAGA
CTATTTAATTCACATCACCACCGGTACCCACGTCGCGCAGATTTGCTGGTTTCTGCTGGCGGAAGCACGTTACCTGCCCG
CCAGGTTAATACAATCTTCGCCACCGCGTAAAAAAGAACAGCCCAATAGCCCTGGTGCGGTGACGATTATCGATCTCGAT
TTAAGCCGTTATAACGCTATTGCCAGCCGCTTTGCCAAAGAACGCCAGCAAACCCTCGATTTCCTCAAATCCGGCATTGC
CACGCGTAACTCCCATTTCAACCGCATGATTGAGCAGATCGAAAAAGTGGCGATCAAATCCCGCGCACCGATCCTGCTTA
ACGGCCCGACCGGTGCGGGTAAGTCATTTCTGGCGCGACGCATCTTTGAATTAAAACAGGCACGGCATCAGTTTAGCGGC
GCGTTTGTGGAAGTGAACTGCGCCACCCTGCGCGGCGATACCGCCATGTCGACGCTGTTTGGTCATGTGAAAGGCGCGTT
CACCGGGGCGCGGGAATCTCGTGAAGGATTATTACGCAGCGCCAACGATGGCATGTTGTTTCTTGATGAGATTGGCGAAC
TGGGCGCAGACGAACAGGCGATGTTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTG
AGCAGTGATTTTCAGCTGATCGCCGGAACGGTGCGCGATTTGCGTCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGACCT
GTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATT
ATGAAGTGGAGCGCCACGCCTCACTCACCGGCGACAGCGTACGTTTTAACACCGAAGCGAGGCGCGCCTGGCTGGCCTTT
GCGACATCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAGCTTTCTGCCAGCGTCACACGCATGGCGACGTTTGCCGA
TAGTGGGCGTATCACCCTTGAAACCGTAGAAGATGAGATAAACCGTCTACGCTACAACTGGCAGGATAGCCGCTCTTCTG
CGCTTAAGCAGCTACTGGGCGCTGAAGCGGAAAATATCGACCTCTTTGATCGGCTGCAACTGGAACACGTTATCGCTATC
TGCCGTCAGGCAAAGTCGCTTTCCGCTGCCGGGCGTGAACTCTTTGATGTTTCGCGCCAGGGCAAAGCCAGCGTCAACGA
CGCGGATCGGCTACGCAAATATCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGCTGA

Upstream 100 bases:

>100_bases
TTGTTTTTAAATAGATAAAATCTTTACGTCCGTAAACGGAGATTTCCCGCAAAGCCAATTCGCCGATAATGAAATATCGT
CTTTTATAAGGATATCTAAG

Downstream 100 bases:

>100_bases
ATATGATGGTCTGTCAGCACCTGCATCACGCTCGCTGGTGGCGGGGCGTCGGTGTAGACGGCATCGACCATGCTGATGCT
GCCCATATTGACCATCGCAT

Product: sigma 54-dependent transcriptional regulator of rtcBA expression

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVIDRLELLHDARSRSLFETLKRDIANVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFEPDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPNSPGAVTIIDLD
LSRYNAIASRFAKERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANDGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFADSGRITLETVEDEINRLRYNWQDSRSSALKQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRELFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS

Sequences:

>Translated_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVIDRLELLHDARSRSLFETLKRDIANVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFEPDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPNSPGAVTIIDLD
LSRYNAIASRFAKERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANDGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFADSGRITLETVEDEINRLRYNWQDSRSSALKQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRELFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
>Mature_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVIDRLELLHDARSRSLFETLKRDIANVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFEPDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPNSPGAVTIIDLD
LSRYNAIASRFAKERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANDGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFADSGRITLETVEDEINRLRYNWQDSRSSALKQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRELFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS

Specific function: Transcriptional repressor of the rtcAB genes. Interacts with sigma-54 [H]

COG id: COG4650

COG function: function code KT; Sigma54-dependent transcription regulator containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789828, Length=532, Percent_Identity=95.8646616541353, Blast_Score=1052, Evalue=0.0,
Organism=Escherichia coli, GI1788905, Length=275, Percent_Identity=35.2727272727273, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788550, Length=232, Percent_Identity=40.0862068965517, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789233, Length=238, Percent_Identity=37.8151260504202, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1790437, Length=249, Percent_Identity=38.9558232931727, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=32.1981424148607, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=36.4754098360656, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082152, Length=263, Percent_Identity=35.361216730038, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787583, Length=247, Percent_Identity=35.2226720647773, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790299, Length=241, Percent_Identity=35.2697095435685, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786524, Length=239, Percent_Identity=35.5648535564854, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081872, Length=242, Percent_Identity=32.6446280991736, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081858, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078
- InterPro:   IPR009715
- InterPro:   IPR017183 [H]

Pfam domain/function: PF06956 RtcR; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60296; Mature: 60296

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVIDRLELLHDARSRSLFETLK
CCCEEHHHHHHHHHHHCCCCCHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
RDIANVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFEPDKEDYLIHITTGTHVAQ
HHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPNSPGAVTIIDLDLSRYNAIASRFAKERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANDGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHHCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFADSGRITLETVEDEINRLRYNWQDSRSSALKQLLGAEAENIDLFDRLQLEHVIAI
HHHHHCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRELFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVIDRLELLHDARSRSLFETLK
CCCEEHHHHHHHHHHHCCCCCHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
RDIANVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFEPDKEDYLIHITTGTHVAQ
HHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPNSPGAVTIIDLDLSRYNAIASRFAKERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANDGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHHCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFADSGRITLETVEDEINRLRYNWQDSRSSALKQLLGAEAENIDLFDRLQLEHVIAI
HHHHHCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRELFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3045764; 7984428; 7920643; 9738023 [H]