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The map label for this gene is yhdJ [H]

Identifier: 218550546

GI number: 218550546

Start: 3327810

End: 3328709

Strand: Direct

Name: yhdJ [H]

Synonym: EFER_3241

Alternate gene names: 218550546

Gene position: 3327810-3328709 (Clockwise)

Preceding gene: 218550545

Following gene: 218550547

Centisome position: 72.52

GC content: 45.89

Gene sequence:

>900_bases
GTGTCACACTGGGGAGTGACCATGAGATCAAAATGTGAACCAACCTGGTTTGGTGATGAGTCCAGGCGCATTATCCACGG
TGACGCGCTTTCTGAATTAAAAAAACTTCCTTCTGAAAGTGTAGATTTAATTTTTGCTGACCCGCCCTATAACATCGGCA
AAAATTTTGATGGACTGGTTGAGTCCTGGGATGACGCCGATTTTCGCTCCTGGCTGTTTGAAATCATTGCTGAATGCCAC
CGCGTTCTGAAGAAACAAGGCAGCATGTACATCATGAACAGCACGGAAAATATGCCCTGGATCGATCTTGAATGTCGTAA
ACTCTTCACCATTAAAAGCCGCATTATCTGGGCTTATGACAGCTCTGGAGTGCAGGCGAAAAAATACTTCGGCTCAATGT
ATGAACCCATCCTGATGATGGTCAAGGATGCCAAAAACTATACCTTTAACAGTGATGCGATCCTGGTAGAAGCCAAAACC
GGGGCGAAGCGTGCATTGATTGACTACCGTAAAAACCCACCACAGCCATATAACTCGCAAAAAGTTCCAGGGAATGTTTG
GGAATTCTCTCGTGTGCGTTATTTGATGGATGAGTATGAAAACCATCCCACGCAAAAGCCTGTTGCCCTTCTTAAGCGGA
TTATTCTGGCGTCTTCCTGCCCTGGCGATATTGTTCTGGACCCATTCGCGGGTAGCTTCACCACCGGCGAAGTTGCGGTA
GAAACAGGACGTAGATTCATCGGTATTGAAGTTAACTGCGAGTATGTGAAGATGGGGCTTAGAAGATTGAATGTTGACTC
CCATTTTTCTGCCGAAGAGCTGGCGAAAGTGAAAAAACGCAAAACGCGGAATCAGTCGAAAAAAAGTCAAAACTCACGTG
GTTCCCTTTTACCCCGTTAA

Upstream 100 bases:

>100_bases
CTGCGTAAAAAATTGAAAAAGTACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAGGCGCTACTCGGCATG
GGGAAGCGCCTTTTTTATAC

Downstream 100 bases:

>100_bases
CGTAAAAGCATTGTAAGCTCACTTTTCAACTCATAATTTTCGTGTATATTTCCAGCTCATCTGGAGATGAATACGCAGGG
TTGAGCAATGATTCGTAAAT

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSHWGVTMRSKCEPTWFGDESRRIIHGDALSELKKLPSESVDLIFADPPYNIGKNFDGLVESWDDADFRSWLFEIIAECH
RVLKKQGSMYIMNSTENMPWIDLECRKLFTIKSRIIWAYDSSGVQAKKYFGSMYEPILMMVKDAKNYTFNSDAILVEAKT
GAKRALIDYRKNPPQPYNSQKVPGNVWEFSRVRYLMDEYENHPTQKPVALLKRIILASSCPGDIVLDPFAGSFTTGEVAV
ETGRRFIGIEVNCEYVKMGLRRLNVDSHFSAEELAKVKKRKTRNQSKKSQNSRGSLLPR

Sequences:

>Translated_299_residues
MSHWGVTMRSKCEPTWFGDESRRIIHGDALSELKKLPSESVDLIFADPPYNIGKNFDGLVESWDDADFRSWLFEIIAECH
RVLKKQGSMYIMNSTENMPWIDLECRKLFTIKSRIIWAYDSSGVQAKKYFGSMYEPILMMVKDAKNYTFNSDAILVEAKT
GAKRALIDYRKNPPQPYNSQKVPGNVWEFSRVRYLMDEYENHPTQKPVALLKRIILASSCPGDIVLDPFAGSFTTGEVAV
ETGRRFIGIEVNCEYVKMGLRRLNVDSHFSAEELAKVKKRKTRNQSKKSQNSRGSLLPR
>Mature_298_residues
SHWGVTMRSKCEPTWFGDESRRIIHGDALSELKKLPSESVDLIFADPPYNIGKNFDGLVESWDDADFRSWLFEIIAECHR
VLKKQGSMYIMNSTENMPWIDLECRKLFTIKSRIIWAYDSSGVQAKKYFGSMYEPILMMVKDAKNYTFNSDAILVEAKTG
AKRALIDYRKNPPQPYNSQKVPGNVWEFSRVRYLMDEYENHPTQKPVALLKRIILASSCPGDIVLDPFAGSFTTGEVAVE
TGRRFIGIEVNCEYVKMGLRRLNVDSHFSAEELAKVKKRKTRNQSKKSQNSRGSLLPR

Specific function: Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3') [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=285, Percent_Identity=82.8070175438597, Blast_Score=506, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 34340; Mature: 34209

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHWGVTMRSKCEPTWFGDESRRIIHGDALSELKKLPSESVDLIFADPPYNIGKNFDGLV
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHH
ESWDDADFRSWLFEIIAECHRVLKKQGSMYIMNSTENMPWIDLECRKLFTIKSRIIWAYD
HCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHEEEEEEEEEEC
SSGVQAKKYFGSMYEPILMMVKDAKNYTFNSDAILVEAKTGAKRALIDYRKNPPQPYNSQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCC
KVPGNVWEFSRVRYLMDEYENHPTQKPVALLKRIILASSCPGDIVLDPFAGSFTTGEVAV
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEE
ETGRRFIGIEVNCEYVKMGLRRLNVDSHFSAEELAKVKKRKTRNQSKKSQNSRGSLLPR
ECCCEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHWGVTMRSKCEPTWFGDESRRIIHGDALSELKKLPSESVDLIFADPPYNIGKNFDGLV
CCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHH
ESWDDADFRSWLFEIIAECHRVLKKQGSMYIMNSTENMPWIDLECRKLFTIKSRIIWAYD
HCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHEEEEEEEEEEC
SSGVQAKKYFGSMYEPILMMVKDAKNYTFNSDAILVEAKTGAKRALIDYRKNPPQPYNSQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCC
KVPGNVWEFSRVRYLMDEYENHPTQKPVALLKRIILASSCPGDIVLDPFAGSFTTGEVAV
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEE
ETGRRFIGIEVNCEYVKMGLRRLNVDSHFSAEELAKVKKRKTRNQSKKSQNSRGSLLPR
ECCCEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 1459953; 2835774 [H]