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The map label for this gene is sspA [H]

Identifier: 218550505

GI number: 218550505

Start: 3287474

End: 3288112

Strand: Reverse

Name: sspA [H]

Synonym: EFER_3199

Alternate gene names: 218550505

Gene position: 3288112-3287474 (Counterclockwise)

Preceding gene: 218550507

Following gene: 218550504

Centisome position: 71.66

GC content: 52.58

Gene sequence:

>639_bases
ATGGCTGTCGCTGCCAACAAACGTTCGGTAATGACGCTGTTTTCTGGTCCTACTGACATCTATAGCCATCAGGTCCGCAT
TGTGCTGGCTGAAAAAGGTGTTAGCTTTGAGATCGAACACGTGGAAAAGGACAATCCGCCTCAGGATCTGATTGACCTCA
ACCCGAATCAGAGCGTACCGACCCTGGTTGATCGTGAGCTGACCCTGTGGGAATCTCGCATCATTATGGAATACCTTGAT
GAGCGTTTCCCTCATCCGCCGCTGATGCCGGTTTACCCGGTTGCACGTGGTGAAAGCCGCCTGTACATGCACCGTATCGA
AAAAGACTGGTACTCACTGATGAATACCATCATGAACGGTTCTAATGCAGAAGCTGATGCTGCGCGTAAACAGCTGCGCG
AAGAGTTGCTGGCTGTCGCACCAGTTTTCGGTCAGAAGCCTTATTTCCTGAGCGATGAATTCAGCCTGGTAGACTGCTAT
CTGGCACCGCTGCTGTGGCGTCTGCCGCAACTGGGGATTGAGTTCAGCGGTCCGGGCGCGAAAGAGCTGAAAGGCTATAT
GACGCGCGTCTTTGAACGTGATTCCTTCCTCGCTTCCTTAACAGAAGCCGAACGCGAAATGCGCCTCGGTCGGAGTTAA

Upstream 100 bases:

>100_bases
CCGAACATGAATGACGCATCCGCTGGCGGGTAACATCACTTCTGACTGGCCATATGTTGGCTGGTAGCAGTAAAAATTCT
GACTATACCTGGAGGTTTTC

Downstream 100 bases:

>100_bases
TCTGTATGGATTTGTCACAGCTAACACCACGTCGGCCATACCTGCTTCGTGCATTTTACGAATGGCTGCTGGATAACCAG
CTCACACCGCATCTGGTGGT

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLD
ERFPHPPLMPVYPVARGESRLYMHRIEKDWYSLMNTIMNGSNAEADAARKQLREELLAVAPVFGQKPYFLSDEFSLVDCY
LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

Sequences:

>Translated_212_residues
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLD
ERFPHPPLMPVYPVARGESRLYMHRIEKDWYSLMNTIMNGSNAEADAARKQLREELLAVAPVFGQKPYFLSDEFSLVDCY
LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS
>Mature_211_residues
AVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDE
RFPHPPLMPVYPVARGESRLYMHRIEKDWYSLMNTIMNGSNAEADAARKQLREELLAVAPVFGQKPYFLSDEFSLVDCYL
APLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=212, Percent_Identity=97.6415094339623, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24322; Mature: 24191

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVP
CCCCCCCCCEEEEECCCHHHHCCEEEEEEECCCCEEEEEECCCCCCCHHHEECCCCCCCC
TLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYSLMNTIMNG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
SNAEADAARKQLREELLAVAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCEEECCCCH
KELKGYMTRVFERDSFLASLTEAEREMRLGRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVP
CCCCCCCCEEEEECCCHHHHCCEEEEEEECCCCEEEEEECCCCCCCHHHEECCCCCCCC
TLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYSLMNTIMNG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
SNAEADAARKQLREELLAVAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCEEECCCCH
KELKGYMTRVFERDSFLASLTEAEREMRLGRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]