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The map label for this gene is rpoN [H]

Identifier: 218550485

GI number: 218550485

Start: 3264141

End: 3265574

Strand: Direct

Name: rpoN [H]

Synonym: EFER_3179

Alternate gene names: 218550485

Gene position: 3264141-3265574 (Clockwise)

Preceding gene: 218550484

Following gene: 218550486

Centisome position: 71.13

GC content: 50.42

Gene sequence:

>1434_bases
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCTCAGTTACAACAGGCGATTCGTCTGTTGCA
ATTGTCGACGCTGGAACTCCAGCAAGAACTTCAGCAGGCGCTGGAAAGTAACCCCCTGCTCGAGCAAACCGACCTTCACG
AAGAAGTTGATACTCAGCAGCCCCAGGATAGCGAAGCACTGGACACCGCTGACGCACTCGAACAAAAAGAGATGCCAGAG
GAGCTGCCACTCGACGCCAGTTGGGATGAAATCTATACCGCAGGTACGCCATCGGGCACCAGTGGTGATTACATCGATGA
CGAACTCCCGGTCTATCAGGGTGAGACAACCCAGACATTGCAAGATTACCTGATGTGGCAGGTTGAGCTGACGCCCTTTT
CCGATACCGATCGAGCTATTGCGACTTCAATCGTTGATGCTGTTGATGAAACCGGCTATCTCACTGTCTCGCTGGAAGAT
ATTCTCGAAAGCATGGGTGATGAAGAGATCGACATCGAAGAGATCGAAGCCGTTCTCAAACGAATCCAACGTTTTGATCC
TGTCGGTGTCGCAGCAAAAGATCTGCGTGACTGTTTGCTGATCCAACTTTCCCAGTTTGACAAATCAACCCCCTGGCTGG
ATGAAGCCCGATTAATCATCAGCGATCACCTGGATCTACTGGCAAATCATGACTTTCGCAGCTTGATGCGTGTTACGCGG
CTGAAAGAAGATGTGCTGAAAGAAGCGGTTAATTTGATCCAGTCTCTGGATCCCCGTCCCGGTCAGTCGATCCAGACGGG
AGAGCCAGAATATGTGATCCCGGATGTGCTGGTGCGTAAACATAACGGTCACTGGACAGTGGAGCTCAACAGCGACAGTA
TTCCTCGTCTGCAAATTAACCAACAATATGCAGCAATGTGTAATGGTGCACGTAATGACGCCGACAGCCAATTTATTCGC
AGCAACTTACAGGATGCGAAATGGCTTATAAAGAGTCTGGAAAGCCGTAACGACACATTACTGCGCGTCAGCCGCTGCAT
CGTAGAACAACAGCAAGCCTTCTTTGAGCAAGGCGAAGAATTTATGAAACCGATGGTGCTGGCAGATATCGCCGAGGCCG
TCGAAATGCACGAATCGACGATTTCCCGTGTGACCACGCAGAAATATCTGCACAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGCCATGTTAATACCGAAGGCGGAGGCGAAGCCTCATCAACGGCGATTCGGGCGCTGGTGAAGAAACT
GATAGCGGCAGAAAATCCCGCGAAACCTCTGAGTGACAGCAAGTTAGCAACTATGCTTTCTGAACAGGGTATCATGGTGG
CGCGCCGCACCGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAATCAGCGTAAACAACTGGTTTGA

Upstream 100 bases:

>100_bases
TACACGACGAGCACGTTAAGCGTGTATACCTTGGGGAAGACTTCAGACTCTGATAGGGTAGAGAATTGCGACGTTTTAGC
AGGAGAGTACGATTCTGAAC

Downstream 100 bases:

>100_bases
CTCAACCGATAAGGAAGACACTATGCAGCTAAACATCACTGGAAATAACGTCGAAATTACCGATGCTCTGCGCGACTTTG
TCACCACCAAATTCGCAAAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDTQQPQDSEALDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
ILESMGDEEIDIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQQYAAMCNGARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAEAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLATMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Sequences:

>Translated_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDTQQPQDSEALDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
ILESMGDEEIDIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQQYAAMCNGARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAEAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLATMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
>Mature_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDTQQPQDSEALDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
ILESMGDEEIDIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQQYAAMCNGARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAEAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLATMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=94.9685534591195, Blast_Score=936, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53854; Mature: 53854

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDTQQ
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
PQDSEALDTADALEQKEMPEELPLDASWDEIYTAGTPSGTSGDYIDDELPVYQGETTQTL
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLEDILESMGDEEIDIEEIEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLDEARLIISDHLDLLANHDFRSLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHEECCCEEEEEECCCCCCCEEEC
QQYAAMCNGARNDADSQFIRSNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FMKPMVLADIAEAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLATMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDTQQ
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
PQDSEALDTADALEQKEMPEELPLDASWDEIYTAGTPSGTSGDYIDDELPVYQGETTQTL
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLEDILESMGDEEIDIEEIEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLDEARLIISDHLDLLANHDFRSLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHEECCCEEEEEECCCCCCCEEEC
QQYAAMCNGARNDADSQFIRSNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FMKPMVLADIAEAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLATMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]