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The map label for this gene is gadX [H]

Identifier: 218550350

GI number: 218550350

Start: 3115205

End: 3116074

Strand: Direct

Name: gadX [H]

Synonym: EFER_3042

Alternate gene names: 218550350

Gene position: 3115205-3116074 (Clockwise)

Preceding gene: 218550347

Following gene: 218550351

Centisome position: 67.89

GC content: 38.85

Gene sequence:

>870_bases
ATGGAAATGTCACTATCTTTATACATTGCTGCTATTTTAAAGATTATGAAAAACAATACATACCCCGGCGTAATTATCTG
TAGCATCAACGAAACAATACTTACCTTTAATGATGGTAACAAAATCACCATCGCTCCCGGAAATCTAATATTTGTTGATC
CAAGGCAACTAACCATCACTCACTATGGCGAACTTGAAAATGTTGCACTAATTACCCAAAAAACAATTTCGTTATTGGTT
TCCGCATTAAATTCAGATTCCTTAATACATAGCTATAAAGCTCATAATTCACCTTTTATTATTCGAAAATGTCCTGATAT
CGCTATATTTAAATATGCGGCAAATCTGTCACACAAAAGTGACCTCTCCTGCGCTGAAAAACTGCGTAAGCGTTCGTTAA
TGCTGGCCCTGATGTCGCTGTTTCTCGATGATCAGAACTTTATTCCGTTATTAATGCGCATATTGCATCCAGATATTACA
ACTCGCGTTTGTGCTGTCATCAACAGCAATATTGCCAATGACTGGTCACTAACATTAATAGCAAGTGAATTATTAATGAG
CCCAAGCTTATTAAAGAAAAAATTGCAGGCCGAGCAGACGACTTACTCGCAGTTACTCACTGAATGCAGAATGCAGCAGG
CGTTAAAGCTATTACGATGCGACGATTACGCTGTCAAAAAGACAGCAATATTATGCGGTTATAAAAGTGTATCTTACTTT
ATTGCCGTATTTCGTAAGCACTTTGGGATAACGCCAACCGAATATCAGGATAAACGGCAACACCACCATGACCTGAACGC
AACAAAAAGAGTGATGACCTTGCCTGGGAACAGGGCAATAAATGATACCCTGCGCTCCAGATGGTGTTAG

Upstream 100 bases:

>100_bases
CTGGGGAGAGTGTCAACGTTAATATAAAAATAGCCTTTATGTTTTGTTGTATATTGCTCTGGTACATGTGTTAAGTTAAA
GACAATAAGTTTATACGCAC

Downstream 100 bases:

>100_bases
TGAAAAGCGTCAGGGATTAAGGAGATATTAAGATGTCTGCATCACTTATCCGCATTATCTGTGTGATGACGTTATTAGCA
GCATTTCCCACTCGTGCAGA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEMSLSLYIAAILKIMKNNTYPGVIICSINETILTFNDGNKITIAPGNLIFVDPRQLTITHYGELENVALITQKTISLLV
SALNSDSLIHSYKAHNSPFIIRKCPDIAIFKYAANLSHKSDLSCAEKLRKRSLMLALMSLFLDDQNFIPLLMRILHPDIT
TRVCAVINSNIANDWSLTLIASELLMSPSLLKKKLQAEQTTYSQLLTECRMQQALKLLRCDDYAVKKTAILCGYKSVSYF
IAVFRKHFGITPTEYQDKRQHHHDLNATKRVMTLPGNRAINDTLRSRWC

Sequences:

>Translated_289_residues
MEMSLSLYIAAILKIMKNNTYPGVIICSINETILTFNDGNKITIAPGNLIFVDPRQLTITHYGELENVALITQKTISLLV
SALNSDSLIHSYKAHNSPFIIRKCPDIAIFKYAANLSHKSDLSCAEKLRKRSLMLALMSLFLDDQNFIPLLMRILHPDIT
TRVCAVINSNIANDWSLTLIASELLMSPSLLKKKLQAEQTTYSQLLTECRMQQALKLLRCDDYAVKKTAILCGYKSVSYF
IAVFRKHFGITPTEYQDKRQHHHDLNATKRVMTLPGNRAINDTLRSRWC
>Mature_289_residues
MEMSLSLYIAAILKIMKNNTYPGVIICSINETILTFNDGNKITIAPGNLIFVDPRQLTITHYGELENVALITQKTISLLV
SALNSDSLIHSYKAHNSPFIIRKCPDIAIFKYAANLSHKSDLSCAEKLRKRSLMLALMSLFLDDQNFIPLLMRILHPDIT
TRVCAVINSNIANDWSLTLIASELLMSPSLLKKKLQAEQTTYSQLLTECRMQQALKLLRCDDYAVKKTAILCGYKSVSYF
IAVFRKHFGITPTEYQDKRQHHHDLNATKRVMTLPGNRAINDTLRSRWC

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=227, Percent_Identity=48.8986784140969, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1790557, Length=237, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786778, Length=153, Percent_Identity=43.7908496732026, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787776, Length=120, Percent_Identity=44.1666666666667, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786776, Length=129, Percent_Identity=32.5581395348837, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789932, Length=123, Percent_Identity=38.2113821138211, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32793; Mature: 32793

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMSLSLYIAAILKIMKNNTYPGVIICSINETILTFNDGNKITIAPGNLIFVDPRQLTIT
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEECCCEEEEE
HYGELENVALITQKTISLLVSALNSDSLIHSYKAHNSPFIIRKCPDIAIFKYAANLSHKS
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCEEEHHHHCCCCCC
DLSCAEKLRKRSLMLALMSLFLDDQNFIPLLMRILHPDITTRVCAVINSNIANDWSLTLI
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
ASELLMSPSLLKKKLQAEQTTYSQLLTECRMQQALKLLRCDDYAVKKTAILCGYKSVSYF
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEECHHHHHHH
IAVFRKHFGITPTEYQDKRQHHHDLNATKRVMTLPGNRAINDTLRSRWC
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEMSLSLYIAAILKIMKNNTYPGVIICSINETILTFNDGNKITIAPGNLIFVDPRQLTIT
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEECCCEEEEE
HYGELENVALITQKTISLLVSALNSDSLIHSYKAHNSPFIIRKCPDIAIFKYAANLSHKS
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCEEEHHHHCCCCCC
DLSCAEKLRKRSLMLALMSLFLDDQNFIPLLMRILHPDITTRVCAVINSNIANDWSLTLI
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
ASELLMSPSLLKKKLQAEQTTYSQLLTECRMQQALKLLRCDDYAVKKTAILCGYKSVSYF
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEECHHHHHHH
IAVFRKHFGITPTEYQDKRQHHHDLNATKRVMTLPGNRAINDTLRSRWC
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA