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The map label for this gene is gadW [H]

Identifier: 218550349

GI number: 218550349

Start: 3113803

End: 3114540

Strand: Reverse

Name: gadW [H]

Synonym: EFER_3041

Alternate gene names: 218550349

Gene position: 3114540-3113803 (Counterclockwise)

Preceding gene: 218550356

Following gene: 218550348

Centisome position: 67.87

GC content: 42.55

Gene sequence:

>738_bases
ATGGCGCATGTCTGTTCTGTCGTTCTGGTTTGTCGTTCCTTGGACATATACTATGAAAAAAAACATCTTAACATAATAAA
AGACAGTCTCATTCTGATGGATGAGAAACTGGCATTTAGAATCCCACATAATTGTTCCTGGGTGCGTTCACTGGAAATAG
ATGAATGCATTGTGAGCAATTATCTACAACATATTCGCCACCCTGCGCAATATAGCGGCAAACATACCAGACATCGTCTA
CTCATCAATCACCAGCCACCGTTACCGCTGGTAAGGGCAATCTTTGACAGTTTTGACGCTGCAGAAACGTTGACACCGAC
GCTGAGTAATATGCTCTACTTCTCCTGTTTATCACTGTTTTCGATGCATGATGAGTTGGTGCCTCTGCTATTTAACAGTA
TCAACACCTTATCGGGGAAAGTGGAGCGAATAATTAGCGTCGATACTTCCAGACGCTGGTATCTGCGTGATGTGGCAGAA
AAACTCTACACCAGTGAGAGCTTAATCAAAAAAAAGTTGCAGGACGAAAACACTTCTTTCAGTAAAATCTTACTGGCATC
CCGAATGGCAAGAGCAAAAACTCTCCTTGAGCTTAATCAAATGCCATTAACGGTAATTGCTGAGAAATGCGGCTATAGCA
GTACATCCTATTTTATCAATACTTTCCGCCAGTATTATGGCGTGACTCCTCATCAGTATAGCCATCGTTCGGCGGTGTCG
TTCGTATCCTTGTGTTAA

Upstream 100 bases:

>100_bases
ACTAAATATGGCAGTTTTTAAATTACTCTACATGTATACTGTCATTTTGTAAGTATACTCAATATGCATTAGTCACGGGA
ATGAATTAAAAGGGTTTAAA

Downstream 100 bases:

>100_bases
GCCAGCGTATTTCAGTGCCGTGATAGTGGGAATAATCAAATTAGCACTCCTCGGCATCACTGCGATCTCAGTTTGTATGG
TATTGCTCAATCTTATTCTT

Product: AraC-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAHVCSVVLVCRSLDIYYEKKHLNIIKDSLILMDEKLAFRIPHNCSWVRSLEIDECIVSNYLQHIRHPAQYSGKHTRHRL
LINHQPPLPLVRAIFDSFDAAETLTPTLSNMLYFSCLSLFSMHDELVPLLFNSINTLSGKVERIISVDTSRRWYLRDVAE
KLYTSESLIKKKLQDENTSFSKILLASRMARAKTLLELNQMPLTVIAEKCGYSSTSYFINTFRQYYGVTPHQYSHRSAVS
FVSLC

Sequences:

>Translated_245_residues
MAHVCSVVLVCRSLDIYYEKKHLNIIKDSLILMDEKLAFRIPHNCSWVRSLEIDECIVSNYLQHIRHPAQYSGKHTRHRL
LINHQPPLPLVRAIFDSFDAAETLTPTLSNMLYFSCLSLFSMHDELVPLLFNSINTLSGKVERIISVDTSRRWYLRDVAE
KLYTSESLIKKKLQDENTSFSKILLASRMARAKTLLELNQMPLTVIAEKCGYSSTSYFINTFRQYYGVTPHQYSHRSAVS
FVSLC
>Mature_244_residues
AHVCSVVLVCRSLDIYYEKKHLNIIKDSLILMDEKLAFRIPHNCSWVRSLEIDECIVSNYLQHIRHPAQYSGKHTRHRLL
INHQPPLPLVRAIFDSFDAAETLTPTLSNMLYFSCLSLFSMHDELVPLLFNSINTLSGKVERIISVDTSRRWYLRDVAEK
LYTSESLIKKKLQDENTSFSKILLASRMARAKTLLELNQMPLTVIAEKCGYSSTSYFINTFRQYYGVTPHQYSHRSAVSF
VSLC

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=241, Percent_Identity=61.8257261410788, Blast_Score=307, Evalue=5e-85,
Organism=Escherichia coli, GI1786776, Length=238, Percent_Identity=37.3949579831933, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI1787776, Length=234, Percent_Identity=31.6239316239316, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1789933, Length=128, Percent_Identity=40.625, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786778, Length=212, Percent_Identity=33.9622641509434, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790557, Length=123, Percent_Identity=40.650406504065, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28325; Mature: 28194

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVCSVVLVCRSLDIYYEKKHLNIIKDSLILMDEKLAFRIPHNCSWVRSLEIDECIVSN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHCCCHHHHHHHH
YLQHIRHPAQYSGKHTRHRLLINHQPPLPLVRAIFDSFDAAETLTPTLSNMLYFSCLSLF
HHHHHCCCHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMHDELVPLLFNSINTLSGKVERIISVDTSRRWYLRDVAEKLYTSESLIKKKLQDENTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SKILLASRMARAKTLLELNQMPLTVIAEKCGYSSTSYFINTFRQYYGVTPHQYSHRSAVS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
FVSLC
HHHCC
>Mature Secondary Structure 
AHVCSVVLVCRSLDIYYEKKHLNIIKDSLILMDEKLAFRIPHNCSWVRSLEIDECIVSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHCCCHHHHHHHH
YLQHIRHPAQYSGKHTRHRLLINHQPPLPLVRAIFDSFDAAETLTPTLSNMLYFSCLSLF
HHHHHCCCHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMHDELVPLLFNSINTLSGKVERIISVDTSRRWYLRDVAEKLYTSESLIKKKLQDENTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SKILLASRMARAKTLLELNQMPLTVIAEKCGYSSTSYFINTFRQYYGVTPHQYSHRSAVS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
FVSLC
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]