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The map label for this gene is cca [H]

Identifier: 218550309

GI number: 218550309

Start: 3071081

End: 3072319

Strand: Direct

Name: cca [H]

Synonym: EFER_3001

Alternate gene names: 218550309

Gene position: 3071081-3072319 (Clockwise)

Preceding gene: 218550308

Following gene: 218550312

Centisome position: 66.93

GC content: 54.96

Gene sequence:

>1239_bases
GTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGT
CGGCAGTACGCCACAGGAGATGCTCGACGCGGGTTACCAGCAGGTAGGCCGCGATTTTCCTGTTTTTCTGCATCCGCAAA
CGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGATTTACCTGCTATGCCGCACCA
GATGTCACGCTGGAAGATGATCTTAAGCGTCGTGATCTGACCATTAATGCACTAGCCCAGGACGATGACGGTGAGATTAT
CGACCCGTACAATGGTCTGGGCGATCTGCAAAATCGTCTGTTGCGCCATGTTTCCCCCGCTTTTGGCGAAGATCCGTTAC
GTGTACTGCGCGTGGCGCGTTTTGCTGCGCGTTATGCCCACCTCAGTTTTCGTATTGCTGATGAAACTCTGGCGCTGATG
CGCGAGATGACTCACGCGGGTGAACTGGAACACCTGACGCCTGAACGAGTGTGGAAAGAGACGGAAAGTGCCCTCACCAC
CCGCAATCCCCAGGTGTTCTTCCAGGTACTGCACGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCCCTGT
TTGGCGTTCCGGCCCCTGCCAGGTGGCATCCGGAAATCGACACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCG
ATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACGTTATGCCACGATCTCGGTAAAGGACTGACGCCGCCAGAGCTTTG
GCCGCGTCATCACGGCCATGGACCAGCGGGTGTGAAATTAGTGGAACAACTATGTCAGCGTCTGCGCGTACCAAATGAAA
TTCGCGATTTAGCCAAACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCGAAAACCATTGTT
AAATTGTTTGATTCCATTGACGCCTGGCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCG
TGGCAGAACCGGTTTTGAGTCAGTAGATTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGC
CGACAAAAGACGTCGTTGAAGCGGGATTTAAAGGTGTGGAGATTCGCGAGGAGTTGACCCGCCGCCGGATTGCAGCGGTA
GCCAACTGGAAGGAACAACGCTGCCCAAAGTCTAATTAA

Upstream 100 bases:

>100_bases
CCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTA
TTTGACGAAGGGATGATGGC

Downstream 100 bases:

>100_bases
GCATTTGAATTTCAATATATTGCAGACACCGGCCAGGTCGGATAAGACGCACAAGCGTCATATCCGACACTATTCCTCAA
TCAAAAGAACACGACATACA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDDGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLHDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLAKLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESVDYPQGRWLREAWEVAQSVPTKDVVEAGFKGVEIREELTRRRIAAV
ANWKEQRCPKSN

Sequences:

>Translated_412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDDGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLHDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLAKLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESVDYPQGRWLREAWEVAQSVPTKDVVEAGFKGVEIREELTRRRIAAV
ANWKEQRCPKSN
>Mature_412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDDGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLHDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLAKLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESVDYPQGRWLREAWEVAQSVPTKDVVEAGFKGVEIREELTRRRIAAV
ANWKEQRCPKSN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=231, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=98.0487804878049, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=323, Percent_Identity=27.8637770897833, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281360112, Length=210, Percent_Identity=30.4761904761905, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357337, Length=210, Percent_Identity=30.4761904761905, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46554; Mature: 46554

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDDDGEIIDPYNGLGDLQNRL
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALMREMTHAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TESALTTRNPQVFFQVLHDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLAKL
HHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESVDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWEVAQSVPTKDVVEAGFKGVEIREELTRRRIAAVANWKEQRCPKSN
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDDDGEIIDPYNGLGDLQNRL
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALMREMTHAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TESALTTRNPQVFFQVLHDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLAKL
HHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESVDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWEVAQSVPTKDVVEAGFKGVEIREELTRRRIAAVANWKEQRCPKSN
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA