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The map label for this gene is dsbA [C]

Identifier: 218550289

GI number: 218550289

Start: 3050136

End: 3050804

Strand: Direct

Name: dsbA [C]

Synonym: EFER_2981

Alternate gene names: 218550289

Gene position: 3050136-3050804 (Clockwise)

Preceding gene: 218550288

Following gene: 218550290

Centisome position: 66.47

GC content: 44.69

Gene sequence:

>669_bases
ATGTCAAAAAAATGGATTTCATCACTGTTTAAAAGCGTGCTGTTGACCGCTGCGCTGGCAGTGCCTTTTACCGCTTCTGC
TTTCACCGAAGGCACGGATTATATGGTTCTGGAAAAACCAATTCCGAATGCTGATAAAACGCTGATCAAAGTGTTCAGCT
ACGCGTGCCCATTCTGCTACAAGTACGACAAAGCAGTTACTGGCCCGGTATCTGAGAAAGTGAAAGATCTGGTCGCCTTT
ACCCCTTTCCATCTGGAAACCAAAGGTGAATACGGCAAACAAGCGAGCGAAGTCTTTGCCGTATTGATTGCTAAAGACCA
GGCAGCAGGTATCTCTCTGTTTGATGCAAAATCGCAGTTTAAGAAAGCCAAATTTGCTTACTACGCAGCTTATCACGACA
AAAAAGAGCGCTGGTCTGACGGTAAAGATCCGGCAGCCTTTATCAAAACAGGTCTTGACGCTGCAGGTATGAGCCAGGCT
GATTTTGAAGCTGCTCTGAAAGAGCCAGCTGTTCAGGAAACACTGGAAAAATGGAAAGCATCTTACGATGTTGCCAAAAT
CCAGGGTGTTCCGGCATATGTTGTTAATGGTAAATACTTGATTTACACCAAGAGCATCAAATCCATTGACACTATGGCTG
ACCTTATCCGTGAGTTAGCAAGCAAATAA

Upstream 100 bases:

>100_bases
AGTGGAAATCGACGTGATGTCTGATAAAGCTAACCAGACTCATTACCGTGCACTGTTAGTACGTCCACAACAGATGTTTA
AATAATCAGAGGGAACATAA

Downstream 100 bases:

>100_bases
CAGGGAAACAGGCTATGGGTATCAAGGGAATGTGGAAAGACCTTCGCACTTCACCGGTAGACACTCTGGTGAGATGGCAA
GAGCAACGCTTCTTATGGTT

Product: Thiol:disulfide interchange protein dsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSKKWISSLFKSVLLTAALAVPFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDLVAF
TPFHLETKGEYGKQASEVFAVLIAKDQAAGISLFDAKSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDTMADLIRELASK

Sequences:

>Translated_222_residues
MSKKWISSLFKSVLLTAALAVPFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDLVAF
TPFHLETKGEYGKQASEVFAVLIAKDQAAGISLFDAKSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDTMADLIRELASK
>Mature_221_residues
SKKWISSLFKSVLLTAALAVPFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDLVAFT
PFHLETKGEYGKQASEVFAVLIAKDQAAGISLFDAKSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQAD
FEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDTMADLIRELASK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24508; Mature: 24377

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKWISSLFKSVLLTAALAVPFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDLVAFTPFHLETKGEYGKQASEVFAVLIAKDQAAGISLFDAKSQF
HHCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLEKWKA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDTMADLIRELASK
HCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKWISSLFKSVLLTAALAVPFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDLVAFTPFHLETKGEYGKQASEVFAVLIAKDQAAGISLFDAKSQF
HHCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLEKWKA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDTMADLIRELASK
HCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]