Click here to switch to the map view.

The map label for this gene is epsK [H]

Identifier: 218550230

GI number: 218550230

Start: 2989743

End: 2990720

Strand: Direct

Name: epsK [H]

Synonym: EFER_2922

Alternate gene names: 218550230

Gene position: 2989743-2990720 (Clockwise)

Preceding gene: 218550229

Following gene: 218550231

Centisome position: 65.15

GC content: 55.52

Gene sequence:

>978_bases
ATGATCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCGGTCATGATGCTGGTAACCAT
CACGCTTTCCGGGCGGATGCAGCAGCAACTGGGAAGAACGCGCAGCCAGCAGGAGTACCAGCAGGCGCTGTGGTATAGCG
CCAGTGCAGAAAGCCTGGCGCTGAGCGCACTCAGTCTGAGCCTGAAAAATGAAAAGCGCGTGCATCTGGCGCAGCCGTGG
GCTTCTGGCCCTCGTTTGTTGCCACTGCCGCAGGGGCAAATTGCAGTCACCCTGCGTGACGCTCAGGCCTGCTTTAACCT
GAATGCCCTTGCTCAACCGACAACGGTGTCGCGTCCGCTCGCGGTACAACAACTGATTGCCCTGATCTCACGCCTGGATG
TGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAATTTATTGACGAGGACCGCAGCGTGCAGACGCGTCTG
GGCCGTGAAGACAGCGAGTATCTCGCCCGTTCGGTACCGTTCTACGCCGCCAATCAACCGCTGACCGATATCAGCGAAAT
GCGCGTGGTACAGGGAATAGACGCCGGACTTTATCAAAGACTGAAACCGCTGGTCTGTGCGCTGCCGATGACCCGCCAGC
AAATCAACATCAACACCCTGGACGTCACGCAAAGTGTGATTTTTGAGGCGCTGTTTGACCCGTGGTTAAGCCATGTTCAG
GCGCGGGCGTTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTCGCACAGCCGCTACTCGCTGA
CGTCGATGAGCGTACTAAAAAAAAGCTAAAAACCGTCCTGAGCGTGGACAGCAATTACTTCTGGCTGCGTTCAGATATCA
CCGTGAATGAGATTGAACTGACGATGAATTCGTTAATTGTCCGCATTGGCCCACAACACTTTTCGGTTCTCTGGCATCAG
ACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
CATCAGTGAAGGCGATCCCTGTGGCAGTGCGCATGACTCTGCATTCGCCGCAATGGGGCGAGATTGAACGCATCTGGTTG
TTACGCGGGCCGCAATTATC

Downstream 100 bases:

>100_bases
GTTCCATGCTTGAGATTTTTTTCCCGCTTTGCGCCTCCGACCCCATCCGTTGGCAGCGCCATACACCCGACGTGGAGCAC
GGTATCTGGCCTGACGTTGC

Product: type II secretion protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRLLPLPQGQIAVTLRDAQACFNLNALAQPTTVSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLTDISEMRVVQGIDAGLYQRLKPLVCALPMTRQQININTLDVTQSVIFEALFDPWLSHVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKKLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRIGPQHFSVLWHQ
TGESE

Sequences:

>Translated_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRLLPLPQGQIAVTLRDAQACFNLNALAQPTTVSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLTDISEMRVVQGIDAGLYQRLKPLVCALPMTRQQININTLDVTQSVIFEALFDPWLSHVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKKLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRIGPQHFSVLWHQ
TGESE
>Mature_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRLLPLPQGQIAVTLRDAQACFNLNALAQPTTVSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLTDISEMRVVQGIDAGLYQRLKPLVCALPMTRQQININTLDVTQSVIFEALFDPWLSHVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKKLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRIGPQHFSVLWHQ
TGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=239, Percent_Identity=38.4937238493724, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36585; Mature: 36585

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRLLPLPQGQIAVTLRDAQACFNLNALAQPTTVSRPL
HHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEEEEEECHHHHHCCCCCCCCCCCCCHH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LTDISEMRVVQGIDAGLYQRLKPLVCALPMTRQQININTLDVTQSVIFEALFDPWLSHVQ
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKKLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRIGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRLLPLPQGQIAVTLRDAQACFNLNALAQPTTVSRPL
HHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEEEEEECHHHHHCCCCCCCCCCCCCHH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LTDISEMRVVQGIDAGLYQRLKPLVCALPMTRQQININTLDVTQSVIFEALFDPWLSHVQ
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKKLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRIGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]