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The map label for this gene is rspA [C]

Identifier: 218550182

GI number: 218550182

Start: 2938487

End: 2939644

Strand: Reverse

Name: rspA [C]

Synonym: EFER_2872

Alternate gene names: 218550182

Gene position: 2939644-2938487 (Counterclockwise)

Preceding gene: 218550183

Following gene: 218550181

Centisome position: 64.06

GC content: 44.3

Gene sequence:

>1158_bases
TTGTATGGGCTCGGATGCGCTACGTTTACTCAGCGGATAGTGGCAGTGGAAAAAGCAGTAAATGAGTATATGAAGCCTTT
CTTGATTGGGAAAAATCCTGCTCGTATAGAAGATATCTGGCAATCAGCATGTGTCAGCGGCTACTGGCGTAATGGCCCAA
TTATGAATAATGCCTTGTCAGGGATCGATATGGCTTTATGGGATCTAAAGGGAAAAGTCGCCGGACTCCCTGTTTATGAA
TTACTGGGGGGGAAATGCCGTGATGGCGTGCCATTATACCGCCATACTGATGGGGCTGATGAGATTGAGGTTGAAGATAA
TATTCACCGCGCTATCGCAGAAGGGTATCAGTATGTTCGTTGTCAGATGGGGATGTATGGTGGGGCTGGAACCGATGATC
TGAAACTTATTGCCAGTAAATTAGCCAGAGCTAAAAATATTCAACCGAAACGTTCACCTACCAATAAAACTTCAGGCATC
TATTTTGACCCAGAAGCGTATGCCAGAAGTGTTCCTCGTTTGTTCGATCATTTGCGTAATAAGTTTGGTTTTGGCATTGA
ATTTATCCATGACGTTCATGAACGTATAAGTCCCATCAACGCAATTCAATTAGCAAAATCACTCGAACCTTACTCACTTT
TTTATCTTGAAGATCCCGTAGCCCCAGAAAATCTTGAGTGGTTGAAGTTAATGCGTCAGCAATCATCAACACCTATTGCA
ATGGGGGAATTGTTCACGAATGTTAATGAATATAAGCCATTAATAATTAATCAACAGATCGATTTTATTCGCTGCCATGT
AAGTACAATTGGCGGGATCACTCCAGCAAGAAAACTGGCGATTTTGAGCGAGATGTTCGGTGTTCGAACAGCATGGCATG
GTCCAGGGGATATTTCTCCGGTAGGGGTAGCCGCTAATATGCATTTGTCAGTAAGTTCTGGCAATTTTGGTATTCAGGAA
TACACGCCAGTCAATGAGGCTTTGCAAGAGGTATTTACAGGCTGCGTTCAGGTTAAGGACGGATTTGCCTATGTGAGTGA
TAAACCAGGGCTGGGTATCGAACTGGATGAACATAAAGCAAAAGCATGGCCAATTAATGGCGGTATTCCAGGCTGGACAT
TAGCGCGTACCCCTGACGGTACGGCTGCCCGACCATAG

Upstream 100 bases:

>100_bases
ATATTGAATATGTGACCAAATAACGGATGTCAGAACGATCCTCACTGCACCCGGCGGGATCGAACTGGCAGTTGTAAAAA
TTGAAACAAATGAGCCAGGT

Downstream 100 bases:

>100_bases
CCGTAGATTTATTTTTGACGTTATCTCTCATCAAGGGGAACTGTAACCTTTACCCTGACTTACTTGAACTCGTGCAAATT
ACCAGCGATAATTCTTCTTA

Product: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MYGLGCATFTQRIVAVEKAVNEYMKPFLIGKNPARIEDIWQSACVSGYWRNGPIMNNALSGIDMALWDLKGKVAGLPVYE
LLGGKCRDGVPLYRHTDGADEIEVEDNIHRAIAEGYQYVRCQMGMYGGAGTDDLKLIASKLARAKNIQPKRSPTNKTSGI
YFDPEAYARSVPRLFDHLRNKFGFGIEFIHDVHERISPINAIQLAKSLEPYSLFYLEDPVAPENLEWLKLMRQQSSTPIA
MGELFTNVNEYKPLIINQQIDFIRCHVSTIGGITPARKLAILSEMFGVRTAWHGPGDISPVGVAANMHLSVSSGNFGIQE
YTPVNEALQEVFTGCVQVKDGFAYVSDKPGLGIELDEHKAKAWPINGGIPGWTLARTPDGTAARP

Sequences:

>Translated_385_residues
MYGLGCATFTQRIVAVEKAVNEYMKPFLIGKNPARIEDIWQSACVSGYWRNGPIMNNALSGIDMALWDLKGKVAGLPVYE
LLGGKCRDGVPLYRHTDGADEIEVEDNIHRAIAEGYQYVRCQMGMYGGAGTDDLKLIASKLARAKNIQPKRSPTNKTSGI
YFDPEAYARSVPRLFDHLRNKFGFGIEFIHDVHERISPINAIQLAKSLEPYSLFYLEDPVAPENLEWLKLMRQQSSTPIA
MGELFTNVNEYKPLIINQQIDFIRCHVSTIGGITPARKLAILSEMFGVRTAWHGPGDISPVGVAANMHLSVSSGNFGIQE
YTPVNEALQEVFTGCVQVKDGFAYVSDKPGLGIELDEHKAKAWPINGGIPGWTLARTPDGTAARP
>Mature_385_residues
MYGLGCATFTQRIVAVEKAVNEYMKPFLIGKNPARIEDIWQSACVSGYWRNGPIMNNALSGIDMALWDLKGKVAGLPVYE
LLGGKCRDGVPLYRHTDGADEIEVEDNIHRAIAEGYQYVRCQMGMYGGAGTDDLKLIASKLARAKNIQPKRSPTNKTSGI
YFDPEAYARSVPRLFDHLRNKFGFGIEFIHDVHERISPINAIQLAKSLEPYSLFYLEDPVAPENLEWLKLMRQQSSTPIA
MGELFTNVNEYKPLIINQQIDFIRCHVSTIGGITPARKLAILSEMFGVRTAWHGPGDISPVGVAANMHLSVSSGNFGIQE
YTPVNEALQEVFTGCVQVKDGFAYVSDKPGLGIELDEHKAKAWPINGGIPGWTLARTPDGTAARP

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=383, Percent_Identity=40.9921671018277, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI48994953, Length=391, Percent_Identity=31.2020460358056, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 42531; Mature: 42531

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGLGCATFTQRIVAVEKAVNEYMKPFLIGKNPARIEDIWQSACVSGYWRNGPIMNNALS
CCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
GIDMALWDLKGKVAGLPVYELLGGKCRDGVPLYRHTDGADEIEVEDNIHRAIAEGYQYVR
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHHHHHEEH
CQMGMYGGAGTDDLKLIASKLARAKNIQPKRSPTNKTSGIYFDPEAYARSVPRLFDHLRN
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
KFGFGIEFIHDVHERISPINAIQLAKSLEPYSLFYLEDPVAPENLEWLKLMRQQSSTPIA
HHCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEE
MGELFTNVNEYKPLIINQQIDFIRCHVSTIGGITPARKLAILSEMFGVRTAWHGPGDISP
HHHHHHCHHHCCCEEECCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHCEECCCCCCCCC
VGVAANMHLSVSSGNFGIQEYTPVNEALQEVFTGCVQVKDGFAYVSDKPGLGIELDEHKA
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCC
KAWPINGGIPGWTLARTPDGTAARP
CCCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MYGLGCATFTQRIVAVEKAVNEYMKPFLIGKNPARIEDIWQSACVSGYWRNGPIMNNALS
CCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
GIDMALWDLKGKVAGLPVYELLGGKCRDGVPLYRHTDGADEIEVEDNIHRAIAEGYQYVR
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHHHHHEEH
CQMGMYGGAGTDDLKLIASKLARAKNIQPKRSPTNKTSGIYFDPEAYARSVPRLFDHLRN
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
KFGFGIEFIHDVHERISPINAIQLAKSLEPYSLFYLEDPVAPENLEWLKLMRQQSSTPIA
HHCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEE
MGELFTNVNEYKPLIINQQIDFIRCHVSTIGGITPARKLAILSEMFGVRTAWHGPGDISP
HHHHHHCHHHCCCEEECCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHCEECCCCCCCCC
VGVAANMHLSVSSGNFGIQEYTPVNEALQEVFTGCVQVKDGFAYVSDKPGLGIELDEHKA
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCC
KAWPINGGIPGWTLARTPDGTAARP
CCCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8277241 [H]