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The map label for this gene is recJ [H]

Identifier: 218550139

GI number: 218550139

Start: 2894929

End: 2896662

Strand: Reverse

Name: recJ [H]

Synonym: EFER_2827

Alternate gene names: 218550139

Gene position: 2896662-2894929 (Counterclockwise)

Preceding gene: 218550140

Following gene: 218550137

Centisome position: 63.13

GC content: 56.81

Gene sequence:

>1734_bases
GTGAAACAACAGATACAACTTCGTCGCCGTGAAGTCGATGAAACGGCAGACTTGCCCGCTGAATTGCCTCCCTTGCTGCG
CCGTTTATATGCCAGCCGGGGAGTACGCAGTGCGCAAGAACTGGAACGTAGTGTAAAAGGTATGCTGCCCTGGCAGCAAC
TGAGCGGCGTCGAAAAGGCCGTTGAGATCCTTTACAACGCTTTTCGCGAAGGAACGCGGATTATGGTGGTCGGTGACTTC
GACGCCGACGGTGCCACCAGCACGGCTCTAAGCGTGCTGGCGATGCGCTCGCTTGGTTGCAGCAATATCGACTATCTGGT
ACCAAACCGTTTCGAAGACGGTTACGGCTTAAGCCCGGAAGTGGTCGATCAGGCCCATGCCCGTGGCGCGCAGTTAATTG
TCACGGTGGATAACGGCATTTCCTCCCATGCGGGGGTTGAACATGCTCGATCGTTGGGCATTCCGGTTATTGTTACCGAT
CACCATTTGCCGGGCGAAACATTACCCGCGGCGGAAGCGATCATTAACCCTAACTTGCGCGACTGCAATTTCCCGTCGAA
ATCACTGGCAGGCGTGGGTGTGGCGTTTTATTTAATGCTGGCGCTGCGCACCTTTTTGCGTGATCAGGGCTGGTTTGATG
AGCGCGGCATCGCAATTCCTAACCTGGCAGAACTGCTGGATCTGGTCGCGTTGGGGACAGTGGCGGACGTCGTGCCGCTG
GATGCTAATAATCGCATTCTGACCTGGCAGGGGATGAGTCGCATCCGTGCCGGAAAGTGCCGTCCAGGGATTAAAGCGCT
GCTGGAAGTGGCCAACCGCGATGCACAAAAACTCGCCGCCAGCGATTTAGGTTTTGCGCTGGGGCCACGTCTCAATGCAG
CCGGGAGACTGGATGATATGTCCGTTGGTGTGGCGCTGTTGCTGTGCGACAACATCGGCGAAGCGCGCGTACTGGCAAAT
GAACTCGATGCGCTAAACCAGACGCGAAAAGAGATCGAACAGGGAATGCAGGTTGAAGCTCTGACCCTGTGCGAGAAACT
GGAGCGCAGCCGCGACACGCTACCCGGCGGGCTGGCGATGTATCACCCGGAATGGCATCAGGGCGTGGTTGGTATTCTGG
CTTCGCGTATTAAAGAGCGTTTTCACCGTCCGGTTATCGCTTTTGCGCCTGCGGGTGACGGTACGCTGAAAGGTTCCGGT
CGCTCCATTCAGGGGCTGCATATGCGTGATGCGCTGGAGCGATTAGACACACTCTACCCCGGCATGATGCTGAAGTTTGG
CGGTCATGCGATGGCGGCAGGCCTGTCGCTGGAAGAGGATAAATTCGAACTCTTTCAGCAACGGTTTGGCGAACTGGTTA
CTGAGTGGCTGGACCCTTCACTATTGCAAGGCGAAGTGGTGTCAGACGGTCCGCTAAGCCCAGCCGAAATGACCATGGAA
GTGGCGCAACTGCTGCGCGATGCTGGCCCGTGGGGGCAGATGTTCCCGGAGCCGCTGTTTGACGGTCATTTCCGTCTGCT
GCAACAGCGGCTGGTGGGCGAGCGTCATTTGAAAGTCATGGTCGAACCGGTCGGCGGCGGTCCGCTGCTGGACGGTATTG
CTTTTAATGTCGATACCGCCCTCTGGCCGGATAACGGCGTGCGCGAAGTGCAACTGGCTTACAAGCTGGATATCAACGAG
TTTCGCGGCAACCGCAGCCTGCAAATTATCATCGACAATATCTGGCCAATTTAG

Upstream 100 bases:

>100_bases
TGCTGAGCAATGGCACACTTGTTCCGGGTTACCAGCCGCCGAAAGAGATGAAAGAATTCCTCGACGAACACCAAAAAATG
ACCAGCGGTAAATAATTCGC

Downstream 100 bases:

>100_bases
CGTCATATTATCTATAAAAAAGAGCGTGGATTGGGTACAATCCCGCTCTTATCACCGCATTTTGACTAGCTCAATAAAAG
AAATCAGACCATGTTTGAAA

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIMVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Sequences:

>Translated_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIMVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI
>Mature_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIMVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=577, Percent_Identity=99.1334488734835, Blast_Score=1160, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63352; Mature: 63352

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIMVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGETLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIMVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGETLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQVEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]