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The map label for this gene is ptsP [H]

Identifier: 218550077

GI number: 218550077

Start: 2826332

End: 2828578

Strand: Reverse

Name: ptsP [H]

Synonym: EFER_2762

Alternate gene names: 218550077

Gene position: 2828578-2826332 (Counterclockwise)

Preceding gene: 218550078

Following gene: 218550076

Centisome position: 61.64

GC content: 51.45

Gene sequence:

>2247_bases
ATGCTCACTCGCCTGCGCGAAATAGTCGAAAAAGTTGCCAGTGCTCCGCGCCTCAATGAGGCGCTGAACATTCTGGTCAC
CGACATCTGTCTTGCGATGAACACCGAAGTCTGTTCGGTCTATCTGGCCGATCATGACCGCCGTTGTTACTACCTTATGG
CGACCCGGGGGCTGAAAAAACCACGTGGTCGCACTGTAGCGCTCGCGTTTGATGAAGGTATCGTCGGCCTGGTTGGCAGG
CTGGCAGAACCTATCAACCTTGCAGATGCGCAAAAACATCCCAGCTTTAAATATGTTCCTTCGGTTAAAGAAGAGCGTTT
TCGCGCCTTCCTTGGCGTGCCAATTATTCAGCGTCGGCAGTTGCTGGGGGTATTAGTCGTCCAGCAGCGGGAAATTCGTC
AATACGACGAAAGCGAAGAATCTTTCCTGGTTACGCTTGCCACCCAAATGGCAGCCATTCTTTCTCAATCGCAATTGACC
GCCTTGTTTGGTCAGTATCGTCAGACACGAATCCGTGCTTTGCCTGCGGCACCGGGCGTAGCGATTGCAGAAGGCTGGCA
GGATGCAACACTGCCATTAATGGAACAGGTTTATCAGGCTTCTACCCTCGACCCGGCGCTGGAGCGTGAGCGCCTGACCG
GAGCACTGGAAGAGGCGGCAAACGAATTTCGCCGTTACAGTAAGCGTTTTTCTGCCGGAGCGCAGAAAGAGACTGCCGCC
ATCTTTGACCTTTATTCCCACTTGCTCTCTGATGCCCGCTTGCGCCGTGAGCTATTTGCCGAAGTGGATAAAGGTTCGGT
TGCTGAGTGGGCAGTGAAAACGGTAATCGAAAAATTTGCCGAGCAGTTTGCTGCACTCACCGATAACTACCTGAAAGAAC
GCGCTGGCGACTTACGTACATTGGGGCAGCGTTTATTATTCCATCTTGACGATTCCGTACAGGCCACCAATGCCTGGCCA
GAGCGCTTTATCCTGGTGGCTGATGAACTTTCTGCGACGACGCTCGCAGAGCTTCCCCAGGATCGTCTGGCAGGTGTTGT
GGTGCGAGATGGCGCAGCAAACTCTCATGCTGCGATTATGGTGCGAGCGTTAGGTATTCCAACTGTCATGGGGGCCGATA
TACAGCCCTCTGTGCTTCATAAACGTACGCTTATTGTTGATGGTTATCGCGGAGAATTATTAGTTGATCCTGAACCGGTT
TTAATTCAGGAATATCAGCGTCTTATCAGCGAAGAAATCGAACTTAGCCGTATTGCCGAAGATGATGTCAATCGCCCGGC
ACAGCTGAAAAGCGGCGAAAGCATTAAAGTCATGCTCAATGCTGGCTTAAGCCCGGAACATGAAGAGAAATTAGGCAGTC
GTATTGACGGTATCGGCCTGTACCGTACCGAAATCCCTTTTATGCTACAAAGCGGTTTTCCATCTGAAGAAGAGCAGGTG
GCGCAGTATCAGGGAATGTTGCAAATGTTCAACGATAAACCTGTTACGTTGCGAACGCTGGATGTTGGTGCAGATAAACA
ACTGCCTTATATGCCCATCAGTGAAGAGAATCCGTGTCTTGGATGGCGGGGGATTCGCATAACCCTCGACCAGCCGGAGA
TCTTTCTGGTACAGGTGCGTGCGATGCTGCGCGCTAATGCTGCCACCGGAAATCTCAGCATTCTACTGCCAATGGTCACC
AGCATTGATGAAGTAGATGAAGCACGTCGCTTAATTGAGCGTGCCGGACGTGAAGTTGAAGAAATGATCGGTTACGCGAT
CCCAAAACCGCGTATTGGTATCATGCTGGAAGTGCCATCAATGATCTTTATGTTGCCGCACCTGGCCAACCGGATCGACT
TTATCTCAGTGGGAACGAACGATCTCACTCAGTACATTCTGGCGGTTGATCGCAACAATACCCGTGTGGCGAATATCTAC
GATAGCCTTCATCCTGGAATGTTGCGGGTGTTGGCGGTAATTGCTCAGGAAGCGGAGAAATACGGTATCGATCTTCGCCT
GTGTGGTGAAATGGCGGGCGATCCGATGTGTGTGGCTATTCTGATTGGTATGGGGTATCGACATCTCTCTATGAATGGTC
GCTCGATTGCCCGCGTGAAGTATCTTCTGCGTAACATCGATTATGACGATGCTAAAAACCTTGCTGAACGGAGTCTGGAA
GCGCAACTGGCAACCGAAGTTCGTCATCAGGTGGCGGCATTTATGGAGCGGCGCGGCTTAGGCGGACTGATACGTGGCGG
GCTTTAA

Upstream 100 bases:

>100_bases
GAAAGAGTTTGCAAGTGTAGTCATGGCGCTTCAGGACAATACGCCGAAGCCACAAAACTCACCTGCTTATCGACGTAAAA
GAGGTTAAGTCACGCAAATC

Downstream 100 bases:

>100_bases
AAGCCCGTCTGTAAAACGTAACGAAGCATTACTTTTACAGCTAATCGGGCAATATGCCCGATCCCTTGTGCTATTATTCG
CACCTTTGGAGCGCCTGTAA

Product: bifunctional phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain-containing protein

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMNTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYVPSVKEERFRAFLGVPIIQRRQLLGVLVVQQREIRQYDESEESFLVTLATQMAAILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFSAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQATNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHKRTLIVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRIAEDDVNRPAQLKSGESIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLVQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMIFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPGMLRVLAVIAQEAEKYGIDLRLCGEMAGDPMCVAILIGMGYRHLSMNGRSIARVKYLLRNIDYDDAKNLAERSLE
AQLATEVRHQVAAFMERRGLGGLIRGGL

Sequences:

>Translated_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMNTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYVPSVKEERFRAFLGVPIIQRRQLLGVLVVQQREIRQYDESEESFLVTLATQMAAILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFSAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQATNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHKRTLIVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRIAEDDVNRPAQLKSGESIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLVQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMIFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPGMLRVLAVIAQEAEKYGIDLRLCGEMAGDPMCVAILIGMGYRHLSMNGRSIARVKYLLRNIDYDDAKNLAERSLE
AQLATEVRHQVAAFMERRGLGGLIRGGL
>Mature_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMNTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYVPSVKEERFRAFLGVPIIQRRQLLGVLVVQQREIRQYDESEESFLVTLATQMAAILSQSQLT
ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFSAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQATNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHKRTLIVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRIAEDDVNRPAQLKSGESIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLVQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMIFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVANIY
DSLHPGMLRVLAVIAQEAEKYGIDLRLCGEMAGDPMCVAILIGMGYRHLSMNGRSIARVKYLLRNIDYDDAKNLAERSLE
AQLATEVRHQVAAFMERRGLGGLIRGGL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=94.6524064171123, Blast_Score=1454, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=581, Percent_Identity=34.4234079173838, Blast_Score=302, Evalue=6e-83,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=32.4701195219124, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=31.7399617590822, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1787994, Length=408, Percent_Identity=25, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI226510935, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83579; Mature: 83579

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMNTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEEEECCCCCC
PRGRTVALAFDEGIVGLVGRLAEPINLADAQKHPSFKYVPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQREIRQYDESEESFLVTLATQMAAILSQSQLTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFSAGAQKETAA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALTDNYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGQRLLFHLDDSVQATNAWPERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHKRTLIVDGYRGELLVDPEPVLIQEYQRLISEEIELSRIAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNRPAQLKSGESIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
HHCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLVQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHH
AMLRANAATGNLSILLPMVTSIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHH
MIFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPGMLRVLAVIAQEAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHEECCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
YGIDLRLCGEMAGDPMCVAILIGMGYRHLSMNGRSIARVKYLLRNIDYDDAKNLAERSLE
HCCCEEEECCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
AQLATEVRHQVAAFMERRGLGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMNTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEEEECCCCCC
PRGRTVALAFDEGIVGLVGRLAEPINLADAQKHPSFKYVPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQREIRQYDESEESFLVTLATQMAAILSQSQLTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFSAGAQKETAA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALTDNYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGQRLLFHLDDSVQATNAWPERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHKRTLIVDGYRGELLVDPEPVLIQEYQRLISEEIELSRIAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNRPAQLKSGESIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
HHCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLVQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHH
AMLRANAATGNLSILLPMVTSIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHH
MIFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPGMLRVLAVIAQEAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHEECCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
YGIDLRLCGEMAGDPMCVAILIGMGYRHLSMNGRSIARVKYLLRNIDYDDAKNLAERSLE
HCCCEEEECCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
AQLATEVRHQVAAFMERRGLGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]