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The map label for this gene is gadW [H]

Identifier: 218550018

GI number: 218550018

Start: 2776597

End: 2777337

Strand: Reverse

Name: gadW [H]

Synonym: EFER_2703

Alternate gene names: 218550018

Gene position: 2777337-2776597 (Counterclockwise)

Preceding gene: 218550019

Following gene: 218550017

Centisome position: 60.53

GC content: 31.85

Gene sequence:

>741_bases
GTGGATAATATGCCATACATATGTTCTATCATTTTGGTGTTGAACTCGTTTGATGCCCGAATTGGTAAAGAAGATATTTT
GTTTAAAAAGGGAAGTGCTGTTCTCATTGATTACAATTTAAAAGATTTTTTTTCATCAAATATAGATCATGTAATGATCG
TAGATGTTGAAGAGAAAACAGTTAATGATTTCTTTAAAAGCAACACACTCTCACCTTTTTCTGTAAGAAGGTTTTATCCG
GCATACTTGATGGTGGAATGTGAAGATTTTTCATTGTTAAAGAACTTGATTGCATGCTTGAATTGTGATGGCAGAACTGT
AGATTTTGTTAGAAATCAAATATCACTTGCATGTCTTGCTATCTTATCTTCAGAGAAAATAGTGCAAAGTTTTTTATTTG
GATGTCTTAATAGTTTAGGAAGTAAAGTTAAGGCTATTATTCACACGGATATATCTGCAGCATGGAGACTTTGTGATATA
TCTTCAAGACTGTATCTGAGTGAAAGTCTGTTAAAAAGAAAATTAAAGCACGAAGGCTTATCATTTAGTAAGTTAATTCT
TGAAGAGCGAATGGTGATGGCGGAAAGGTTATTAAGCTACAATTTATATTCTGTTGGAAAAGTTGCTGAGATATGCGGTT
ATGAAAACACGTCATATTTTGTAAGTGTTTTCAGAAGATATTTTGGTGTTCCTCCCCATCAATATTCATCAAGATTTTTT
TTAGAAAAAGACATGATGTAA

Upstream 100 bases:

>100_bases
TAATTTTATACTTGTGATGCAATGAGATTTTCCTTATTGTTGAACTGGCGAATATTGATTTTCCACCTATACTTACCTGG
TGTAACCTCAATGATATCAG

Downstream 100 bases:

>100_bases
CGTGATGCGTTTTAATGATTTTGTAATTTTCGTATTTGATAATTGTATGATGCTTTCAGCTACGCCAGAATAATCGCTGG
CGTTTTTCTTTTTGAATAGA

Product: araC-type regulatory protein from bacteriophage origin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MDNMPYICSIILVLNSFDARIGKEDILFKKGSAVLIDYNLKDFFSSNIDHVMIVDVEEKTVNDFFKSNTLSPFSVRRFYP
AYLMVECEDFSLLKNLIACLNCDGRTVDFVRNQISLACLAILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDI
SSRLYLSESLLKRKLKHEGLSFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFF
LEKDMM

Sequences:

>Translated_246_residues
MDNMPYICSIILVLNSFDARIGKEDILFKKGSAVLIDYNLKDFFSSNIDHVMIVDVEEKTVNDFFKSNTLSPFSVRRFYP
AYLMVECEDFSLLKNLIACLNCDGRTVDFVRNQISLACLAILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDI
SSRLYLSESLLKRKLKHEGLSFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFF
LEKDMM
>Mature_246_residues
MDNMPYICSIILVLNSFDARIGKEDILFKKGSAVLIDYNLKDFFSSNIDHVMIVDVEEKTVNDFFKSNTLSPFSVRRFYP
AYLMVECEDFSLLKNLIACLNCDGRTVDFVRNQISLACLAILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDI
SSRLYLSESLLKRKLKHEGLSFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFF
LEKDMM

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=234, Percent_Identity=33.7606837606838, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786776, Length=233, Percent_Identity=31.7596566523605, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=32.0675105485232, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789933, Length=101, Percent_Identity=40.5940594059406, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790557, Length=117, Percent_Identity=39.3162393162393, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786778, Length=218, Percent_Identity=29.3577981651376, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28266; Mature: 28266

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNMPYICSIILVLNSFDARIGKEDILFKKGSAVLIDYNLKDFFSSNIDHVMIVDVEEKT
CCCCHHHHHHHHHHHHHHHHCCCHHHEEECCCEEEEECCHHHHHHCCCCEEEEEECCHHH
VNDFFKSNTLSPFSVRRFYPAYLMVECEDFSLLKNLIACLNCDGRTVDFVRNQISLACLA
HHHHHHCCCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDISSRLYLSESLLKRKLKHEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCEE
LEKDMM
EECCCC
>Mature Secondary Structure
MDNMPYICSIILVLNSFDARIGKEDILFKKGSAVLIDYNLKDFFSSNIDHVMIVDVEEKT
CCCCHHHHHHHHHHHHHHHHCCCHHHEEECCCEEEEECCHHHHHHCCCCEEEEEECCHHH
VNDFFKSNTLSPFSVRRFYPAYLMVECEDFSLLKNLIACLNCDGRTVDFVRNQISLACLA
HHHHHHCCCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDISSRLYLSESLLKRKLKHEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCEE
LEKDMM
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]