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The map label for this gene is prpE [H]

Identifier: 218549972

GI number: 218549972

Start: 2731713

End: 2733599

Strand: Reverse

Name: prpE [H]

Synonym: EFER_2657

Alternate gene names: 218549972

Gene position: 2733599-2731713 (Counterclockwise)

Preceding gene: 218549973

Following gene: 218549969

Centisome position: 59.57

GC content: 57.39

Gene sequence:

>1887_bases
ATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGAAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTG
GCAGACGCCCTTTACGCAAACACTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAATTTGT
GTCATAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCATTAATTGCCGTCTCTTCGGAAACAGAAGAA
GAGCGCACCTTTACCTTTCGCCAGTTGCATGACGAAGTGAACGCGGTGGCGTCAATGCTGCGCTCACTGGGCGTACGGCG
TGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACCCTGCTGGCCTGCGCGCGCATTGGCGCTA
TTCACTCGGTAGTGTTTGGTGGATTTGCCTCGCATAGCGTGGCTGCGCGGATTGATGACGCTAAACCGGTGCTGATTGTC
TCGGCAGATGCCGGAGCGCGCGGTGGCAAAATCATTCCTTATAAAAAATTGCTCGACGATGCGATAAGTCAGGCGCAGCA
TCAGCCGCGCCACGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGT
CGTTGCGCCATCAACACATCGGCGCGCGGGTGCCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACC
TCCGGCACGACCGGCAAACCAAAAGGTGTGCAGCGTGACGTCGGCGGTTATGCGGTGGCGCTGGCGACCTCAATGGACAC
CATTTTTGGTGGCAAAGCGGGCGGTGTGTTCTTCTGTGCGTCGGATATCGGCTGGGTAGTTGGGCATTCGTATATCGTCT
ACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACTTATCCTGACTGCGGTGTGTGGTGGAAA
ATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATCCGCGTGCTGAAAAAATTCCCTACCGCTGA
AATTCGCAAACACGATCTTTCCTCGCTGGAAGCACTGTATCTGGCGGGGGAACCGCTGGACGAGCCGACCGCCAGTTGGG
TGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGC
GGTCTGGATGACAGGCCGACGCGTCTGGGAAGCCCCGGCGTGCCGATGTATGGCTACAACGTGCAGTTGCTCAATGAAGT
CACCGGCGAACCGTGCGGCGTCAACGAGAAAGGGATGCTGGTGGTAGAAGGGCCGCTGCCGCCGGGGTGTATTAAAACCA
TCTGGGGCGATGACGACCGCTTTGTGAAGACGTATTGGTCGTTATTTTCCCGTCCGGTGTACGCCACCTTTGACTGGGGC
ATCCGCGATGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTGATTAACGTTGCCGGGCATCGGCTGGGGAC
GCGCGAGATTGAAGAGAGTATCTCCAGTCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCACTGAAAG
GGCAGGTGGCAGTGGCGTTTGTCATTCCGAAAGAAAGCGACAGTCTGGAAGATCGCGAGGTGGCGCACTCGCAAGAGAAG
GCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGTCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTACCGAA
AACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGTGAAGGACGCGATCCGGGGGATCTGACGACTATTG
ATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAA

Upstream 100 bases:

>100_bases
GGACAGAGCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGT
AAGTTCAACAGGAGAGCATT

Downstream 100 bases:

>100_bases
GGCGTTGTCGGATGTGCTCGCGTGACGCATCCGACATTTATCGCCAGCTCACCTGATAATAATAACGACCAGCGTTCAAT
AAAAACTCCGGCGAAACTGA

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEALYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIKTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Sequences:

>Translated_628_residues
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEALYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIKTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE
>Mature_627_residues
SFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEE
RTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVS
ADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYTS
GTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWKI
VEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEALYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARG
LDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIKTIWGDDDRFVKTYWSLFSRPVYATFDWGI
RDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKA
IMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=633, Percent_Identity=39.9684044233807, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI8923896, Length=657, Percent_Identity=35.1598173515982, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=36.71875, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=34.9253731343284, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI115511026, Length=564, Percent_Identity=27.3049645390071, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI42544132, Length=557, Percent_Identity=26.032315978456, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI122937307, Length=586, Percent_Identity=26.962457337884, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI58082049, Length=569, Percent_Identity=26.5377855887522, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI157311624, Length=568, Percent_Identity=26.7605633802817, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI157311622, Length=568, Percent_Identity=26.7605633802817, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI31982927, Length=641, Percent_Identity=23.5569422776911, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI38505220, Length=558, Percent_Identity=25.6272401433692, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI156151445, Length=577, Percent_Identity=22.7036395147314, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI187761345, Length=411, Percent_Identity=24.8175182481752, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI187761343, Length=411, Percent_Identity=24.8175182481752, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786529, Length=628, Percent_Identity=98.5668789808917, Blast_Score=1280, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=637, Percent_Identity=36.5777080062794, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI221142682, Length=543, Percent_Identity=24.3093922651934, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=24.4206773618538, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.1499051233397, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=21.4936247723133, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71982997, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI17531443, Length=549, Percent_Identity=25.3187613843352, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=21.8461538461538, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=568, Percent_Identity=22.7112676056338, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=582, Percent_Identity=22.3367697594502, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=535, Percent_Identity=21.8691588785047, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=543, Percent_Identity=21.3627992633518, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=633, Percent_Identity=39.1785150078989, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323182, Length=641, Percent_Identity=37.597503900156, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319699, Length=407, Percent_Identity=22.6044226044226, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=38.5335413416537, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=34.7692307692308, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=36.1209964412811, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=35.538752362949, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=24.007561436673, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358303, Length=511, Percent_Identity=23.8747553816047, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922652, Length=545, Percent_Identity=23.6697247706422, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356947, Length=375, Percent_Identity=24.5333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=380, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356441, Length=514, Percent_Identity=23.1517509727626, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=22.5433526011561, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20130357, Length=389, Percent_Identity=25.4498714652956, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69398; Mature: 69267

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTYPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEALY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
ECCCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIKTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDREVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTYPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEALY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
ECCCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIKTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDREVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]