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The map label for this gene is aroL

Identifier: 218549950

GI number: 218549950

Start: 2706239

End: 2706784

Strand: Reverse

Name: aroL

Synonym: EFER_2635

Alternate gene names: 218549950

Gene position: 2706784-2706239 (Counterclockwise)

Preceding gene: 218549951

Following gene: 218549949

Centisome position: 58.99

GC content: 53.3

Gene sequence:

>546_bases
ATGATACAACCTCTTTTCCTTGTTGGTCCTCGGGGCTGCGGTAAAACAACCGTCGGCAAAGCACTGGCCGATGCTCTGGA
GCGTCGGTTTGTCGATACCGATCAATGGCTACAGGCCAATGTCCAGATGACAGTGGCTGATATCGTTGAACGTGAAGGCT
GGGCGGGATTTCGCGCCCGGGAAGCAGCAGCTCTTGAAGCGGTTACCGCGCCAGCAACCGTTGTCGCTACTGGCGGAGGG
ATTATTCTCGCGGAACAAAATCGCCACTTCATGCGCAATAACGGAATTGTGATTTACCTGAGCGCCCCGGTAGACGTCCT
GGTTAATCGGCTGGAAGCCGAGCCAGAAGTGGGGCTGCGACCGACATTAACAGGTAAATCACTCAGCGAAGAAGTTGCCG
AAGTGCTTGAGCAGCGCGATATTCTGTACCGTGAAACGGCAAATATTATCGTTGATGCCACGTATGAACCGGGCCAGGTG
ATCTCTGAAATTCGTGCGCTTCTGGATCAAATGGCGTTACGTAATCTGGGTGGGGCCTATACTTAA

Upstream 100 bases:

>100_bases
ATTTTTCTTTACACTTAAATGTGTACTAGTTTGATGGTATAATACATACTTTCCATATTTTACGCTTTCGCGTTTTCGTT
GCACTCTGGGGAACTCAACC

Downstream 100 bases:

>100_bases
TGTTCATCCCGTTAATAAGGAAGAACTATGCCAACAAGACCCCCTTATCCTCGTGAAGCTCGAATTGTGACAGTAGAAAA
AGGAAATCCAGGTCAGACCG

Product: shikimate kinase II

Products: NA

Alternate protein names: SK 2

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MIQPLFLVGPRGCGKTTVGKALADALERRFVDTDQWLQANVQMTVADIVEREGWAGFRAREAAALEAVTAPATVVATGGG
IILAEQNRHFMRNNGIVIYLSAPVDVLVNRLEAEPEVGLRPTLTGKSLSEEVAEVLEQRDILYRETANIIVDATYEPGQV
ISEIRALLDQMALRNLGGAYT

Sequences:

>Translated_181_residues
MIQPLFLVGPRGCGKTTVGKALADALERRFVDTDQWLQANVQMTVADIVEREGWAGFRAREAAALEAVTAPATVVATGGG
IILAEQNRHFMRNNGIVIYLSAPVDVLVNRLEAEPEVGLRPTLTGKSLSEEVAEVLEQRDILYRETANIIVDATYEPGQV
ISEIRALLDQMALRNLGGAYT
>Mature_181_residues
MIQPLFLVGPRGCGKTTVGKALADALERRFVDTDQWLQANVQMTVADIVEREGWAGFRAREAAALEAVTAPATVVATGGG
IILAEQNRHFMRNNGIVIYLSAPVDVLVNRLEAEPEVGLRPTLTGKSLSEEVAEVLEQRDILYRETANIIVDATYEPGQV
ISEIRALLDQMALRNLGGAYT

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family. AroL subfamily

Homologues:

Organism=Escherichia coli, GI1786587, Length=170, Percent_Identity=78.8235294117647, Blast_Score=276, Evalue=5e-76,
Organism=Escherichia coli, GI87082255, Length=160, Percent_Identity=31.875, Blast_Score=86, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320332, Length=155, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROL_ESCF3 (B7LMJ7)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002383741.1
- ProteinModelPortal:   B7LMJ7
- EnsemblBacteria:   EBESCT00000124041
- GeneID:   7121051
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_2635
- GeneTree:   EBGT00050000009881
- HOGENOM:   HBG335815
- ProtClustDB:   PRK03731
- BioCyc:   EFER585054:EFER_2635-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01269
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19690; Mature: 19690

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 34-34 BINDING 58-58 BINDING 79-79 BINDING 120-120 BINDING 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPLFLVGPRGCGKTTVGKALADALERRFVDTDQWLQANVQMTVADIVEREGWAGFRAR
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
EAAALEAVTAPATVVATGGGIILAEQNRHFMRNNGIVIYLSAPVDVLVNRLEAEPEVGLR
HHHHHHHHCCCEEEEEECCEEEEEECCCCEEECCCEEEEECCCHHHHHHHHCCCCCCCCC
PTLTGKSLSEEVAEVLEQRDILYRETANIIVDATYEPGQVISEIRALLDQMALRNLGGAY
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
T
C
>Mature Secondary Structure
MIQPLFLVGPRGCGKTTVGKALADALERRFVDTDQWLQANVQMTVADIVEREGWAGFRAR
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
EAAALEAVTAPATVVATGGGIILAEQNRHFMRNNGIVIYLSAPVDVLVNRLEAEPEVGLR
HHHHHHHHCCCEEEEEECCEEEEEECCCCEEECCCEEEEECCCHHHHHHHHCCCCCCCCC
PTLTGKSLSEEVAEVLEQRDILYRETANIIVDATYEPGQVISEIRALLDQMALRNLGGAY
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA