Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 218549935

GI number: 218549935

Start: 2689167

End: 2690237

Strand: Reverse

Name: queA [H]

Synonym: EFER_2620

Alternate gene names: 218549935

Gene position: 2690237-2689167 (Counterclockwise)

Preceding gene: 218549937

Following gene: 218549934

Centisome position: 58.63

GC content: 53.59

Gene sequence:

>1071_bases
ATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCAATGCCTGAACGCAGTAGCTGTCG
TTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGTACTTTCACCGATTTACTCGATAAGCTCAACCCCGGCG
ATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGTCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTG
CTGGTTGAGCGTATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCGGGCGCAGAGCT
GCTGCTGGGCGATGACGAAAGTATCAACGCAACAATGACCGCTCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATG
ATCGTTCTGTACTGGATATCCTCAACAGCATCGGCCACATGCCACTGCCGCCGTACATCGACCGTCCGGACGAAGATGCA
GACCGCGAACTTTATCAAACAGTTTATAGTGAAAAACCGGGCGCGGTAGCGGCCCCGACTGCCGGGCTGCATTTTGACGA
ACCTTTGCTGGAAAAATTGCGCGCCAAAGGCGTGGAGATGGCGTTCGTAACTCTGCACGTTGGTGCGGGTACTTTCCAGC
CGGTGCGCGTCGACACCATTGAAGATCACATCATGCACTCAGAATACGCTGAAGTGCCGCAGGATGTGGTAGACGCGGTA
CTGGCAACAAAAGCACGCGGTAATCGGGTGATTGCGGTTGGCACCACCTCGGTACGTTCGCTGGAAAGCGCGGCCCAGGC
GGCGAAAAACGATCTCATCGAACCGTTCTTTGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCG
ATGCGCTGGTGACGAACTTCCACCTTCCTGAATCGACGCTGATTATGCTGGTTTCGGCATTTGCCGGCTATCAACACACT
ATGAACGCCTATAAAGCGGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCACGTACAATCC
GCAGGCAATTAATGAGCGCGTCGGGGAGTAA

Upstream 100 bases:

>100_bases
AAATTCATCGATTATATTCTATCCAAAGGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTA
TTTTAGTCTGAGTCAGTGTC

Downstream 100 bases:

>100_bases
TTCCGCGGCGCTGGTTTAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGA
CGGTCGCGCACGCCGTGGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LATKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE

Sequences:

>Translated_356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LATKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
>Mature_356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LATKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=99.438202247191, Blast_Score=726, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39447; Mature: 39447

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEE
ARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHH
LATKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHHHHHCCC
>Mature Secondary Structure
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEE
ARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHH
LATKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA