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The map label for this gene is mamA [H]

Identifier: 218549698

GI number: 218549698

Start: 2433018

End: 2433518

Strand: Direct

Name: mamA [H]

Synonym: EFER_2375

Alternate gene names: 218549698

Gene position: 2433018-2433518 (Clockwise)

Preceding gene: 218549697

Following gene: 218549699

Centisome position: 53.02

GC content: 44.91

Gene sequence:

>501_bases
GTGCAAACAACCATTTATTTAATGTTTTCACGCCTATACAGAGGATTTGCCATGAAAAAACCAACGCTCGTTATTGGCGT
CATTGGCGCTGACTGCCATGCTGTTGGCAATAAAGTTCTGGATAGAGTTTTCACCGTGCATAACTTTAATGTCATTAACC
TTGGCGTAATGGTGAGTCAGGATGAATATATTGACGCAGCGATAGAAACGGGTGCCGATGCCATTGTGGTTTCCTCTATT
TATGGTCATGGTGATATTGACTGTCTGGGACTTCGTGAGCGCTGCATCGAACGTGGTATCGGAGAAATTCCACTTTATGT
TGGCGGCAATCTGGTGGTTGGCAAACATGACTTTGCTGATGTCGAAAAAAAATTCAAAGAGATGGGATTTAACCGAGTAT
TCGCGCCAAGTCATGACCTGGAAGATGTCTGTCAGCTCATGGCGGGTGATATTCAGCAACACCGGCACGCTGAAGAACAT
GTTCTGGAAGAGGCTATCTGA

Upstream 100 bases:

>100_bases
TCAATATTTTTACGAATAAGATATTGAAAAGAGATCAATTGCATCCTTTATACCTAACACACACACTCACGGATAAATAT
ATCGCACTTACAATAACAGA

Downstream 100 bases:

>100_bases
TGAGAACTGTCTCTGTCGATATCGGTTCGACGTGGACTAAAGCCGCTCTCTTCGGCAGAGAAGGTGATACGTTAACGCTG
ATAAATCACGTGATAACCCC

Product: methylaspartate mutase subunit S

Products: NA

Alternate protein names: Glutamate mutase subunit sigma [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MQTTIYLMFSRLYRGFAMKKPTLVIGVIGADCHAVGNKVLDRVFTVHNFNVINLGVMVSQDEYIDAAIETGADAIVVSSI
YGHGDIDCLGLRERCIERGIGEIPLYVGGNLVVGKHDFADVEKKFKEMGFNRVFAPSHDLEDVCQLMAGDIQQHRHAEEH
VLEEAI

Sequences:

>Translated_166_residues
MQTTIYLMFSRLYRGFAMKKPTLVIGVIGADCHAVGNKVLDRVFTVHNFNVINLGVMVSQDEYIDAAIETGADAIVVSSI
YGHGDIDCLGLRERCIERGIGEIPLYVGGNLVVGKHDFADVEKKFKEMGFNRVFAPSHDLEDVCQLMAGDIQQHRHAEEH
VLEEAI
>Mature_166_residues
MQTTIYLMFSRLYRGFAMKKPTLVIGVIGADCHAVGNKVLDRVFTVHNFNVINLGVMVSQDEYIDAAIETGADAIVVSSI
YGHGDIDCLGLRERCIERGIGEIPLYVGGNLVVGKHDFADVEKKFKEMGFNRVFAPSHDLEDVCQLMAGDIQQHRHAEEH
VLEEAI

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006394 [H]

Pfam domain/function: PF02310 B12-binding [H]

EC number: =5.4.99.1 [H]

Molecular weight: Translated: 18435; Mature: 18435

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTIYLMFSRLYRGFAMKKPTLVIGVIGADCHAVGNKVLDRVFTVHNFNVINLGVMVSQ
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEECC
DEYIDAAIETGADAIVVSSIYGHGDIDCLGLRERCIERGIGEIPLYVGGNLVVGKHDFAD
CHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHCCCCCCCEEECCEEEECCCCHHH
VEKKFKEMGFNRVFAPSHDLEDVCQLMAGDIQQHRHAEEHVLEEAI
HHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTTIYLMFSRLYRGFAMKKPTLVIGVIGADCHAVGNKVLDRVFTVHNFNVINLGVMVSQ
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEECC
DEYIDAAIETGADAIVVSSIYGHGDIDCLGLRERCIERGIGEIPLYVGGNLVVGKHDFAD
CHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHCCCCCCCEEECCEEEECCCCHHH
VEKKFKEMGFNRVFAPSHDLEDVCQLMAGDIQQHRHAEEHVLEEAI
HHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]