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The map label for this gene is bcr [H]

Identifier: 218549598

GI number: 218549598

Start: 2332483

End: 2333673

Strand: Reverse

Name: bcr [H]

Synonym: EFER_2272

Alternate gene names: 218549598

Gene position: 2333673-2332483 (Counterclockwise)

Preceding gene: 218549599

Following gene: 218549597

Centisome position: 50.86

GC content: 52.73

Gene sequence:

>1191_bases
GTGACCACTCGACAGCACTCATCGTTTGCTATTGTTTTTATCCTTGGCCTGTTGGCCATGTTGATGCCGCTGTCGATTGA
TATGTATCTGCCCGCGCTACCGGTAATTTCAGCGCAGTTTGGCGTACCGGCAGGTAGTACGCAGATGACCCTCAGTACTT
ATATTCTGGGGTTTGCGTTAGGGCAGTTAATCTACGGGCCGATGGCAGACAGCTTCGGGCGTAAGCCGGTGGTTCTTGGC
GGTACGCTGGTGTTTGCCGCCGCCGCGGTGGCGTGTGCGCTGGCACAAACCATCGATCAGCTGATTGTGATGCGTTTCTT
CCACGGCCTGGCGGCAGCTGCGGCCAGCGTGGTCATTAACGCCCTGATGCGCGATATCTACCCGAAAGAAGAGTTCTCGC
GGATGATGTCGTTTGTCATGCTGGTGACCACTATTGCACCGTTGATGGCACCGATTGTCGGCGGCTGGGTGCTGGTATGG
TTAAGCTGGCATTACATCTTCTGGATCCTGGCATTGGCGGCGATTCTGGCTTCGGCAATGATTTTCTTCCTGATTAAAGA
AACGTTGCCGCCAGAGCGCCGTCAGCCATTTCACATTCGTACCACTATTGGTAACTTTGCGGCACTGTTCCGCCATAAGC
GTGTCCTGAGTTATATGCTGGCCAGTGGTTTCAGCTTTGCCGGGATGTTCTCATTCTTAAGCGCCGGACCGTTTGTCTAC
ATTGAAATTAACCACGTCGCGCCGGAAAATTTTGGTTATTACTTTGCGCTGAACATTGTTTTCCTGTTTGTGATGACCAT
CTTTAACAGCCGCTTTGTTCGTCGTATTGGCGCGTTAAATATGTTTCGGGCAGGATTATGGATCCAGTTTATGGTGGCGG
CATGGATGGTCATTAGCGCGCTGCTGGGCCTGGGGTTCTGGTCTCTGGTGGTTGGTGTTGCGGCGTTTGTGGGCTGCGTG
TCGATGGTGTCATCCAATGCGATGGCAGTCATTCTGGATGAGTTTCCACACATGGCAGGAACGGCATCGTCACTGGCAGG
CACTTTCCGCTTTGGTATTGGCGCCATTGTTGGTGCATTGCTATCGCTGGCAACCTTCAACTCCGCATGGCCAATGATCT
GGTCAATCGCTCTCTGTGCGACGTGTTCAATTCTCTTCTGTTTGTACGCCAGTCGACCGAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TACCCTGGATGCAGATTTAGCCCCCGGTGAATATCGTCCGTTAACTGAAGAAGAAGTTGCCAGCGTCGTCTAACACTTTA
ATGACTTTCAGGAGCCCGTT

Downstream 100 bases:

>100_bases
TCAATTGCACAATATGGGGGCCGTAAGGCCCCCTTTGTTGATGCATATCAACGACAAATTCATCATTTCTCCGCCTTTTG
TTTGATTTATGTAAAATCAA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRAGLWIQFMVAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIALCATCSILFCLYASRPKKR

Sequences:

>Translated_396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRAGLWIQFMVAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIALCATCSILFCLYASRPKKR
>Mature_395_residues
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGG
TLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWL
SWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYI
EINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRAGLWIQFMVAAWMVISALLGLGFWSLVVGVAAFVGCVS
MVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIALCATCSILFCLYASRPKKR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=98.4848484848485, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=359, Percent_Identity=28.6908077994429, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790146, Length=226, Percent_Identity=30.9734513274336, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082312, Length=339, Percent_Identity=26.2536873156342, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787065, Length=253, Percent_Identity=24.1106719367589, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790794, Length=230, Percent_Identity=20.8695652173913, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081738, Length=357, Percent_Identity=25.4901960784314, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787880, Length=209, Percent_Identity=30.1435406698565, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=25.6281407035176, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=26.6272189349112, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325414, Length=202, Percent_Identity=23.7623762376238, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319517, Length=205, Percent_Identity=22.9268292682927, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=182, Percent_Identity=22.5274725274725, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43319; Mature: 43188

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRAGLWIQFMVAAWMVISA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFNSAWPMIWSIALCATCSILFCLYASRPKKR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRAGLWIQFMVAAWMVISA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFNSAWPMIWSIALCATCSILFCLYASRPKKR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]