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The map label for this gene is galS [H]

Identifier: 218549564

GI number: 218549564

Start: 2293302

End: 2294360

Strand: Reverse

Name: galS [H]

Synonym: EFER_2236

Alternate gene names: 218549564

Gene position: 2294360-2293302 (Counterclockwise)

Preceding gene: 218549565

Following gene: 218549563

Centisome position: 50.0

GC content: 51.09

Gene sequence:

>1059_bases
GTGGCCTACTGCAATTTTCAGAACAGGGTAGTGAATATGATCACCATACGTGATGTGGCTCGTCAGGCTGGCGTCTCCGT
AGCTACGGTTTCCAGGGTGCTAAACAACAGTGTATTAGTCAGCCCTGACACGCGTGAAGCAGTAATGAAAGCGGTAACGC
TACTGGATTACCGTCCGAACGCCAATGCTCAGGCGCTGGCGACGCAGATCAGCGAAACCATCGGCGTGGTGGTGATGGAC
GTGTCAGATGCCTTTTTCGGAGCCCTGGTCAAGGCCGTGGATCTCGTTGCCCAGCAGCACCAGAAATATGTACTGATTGG
CAACAGCTACCATGAAGCAGAAAAAGAGCGCCATGCCATTGAGGTGTTAATTCGTCAACGCTGTAATGCTTTGATTGTTC
ATTCAAAAGCGCTCAGTGATGAGGAACTGGTCCAGTTTATGGAGCAAATACCCGGAATGGTGTTGATTAATCGTCTGGTG
CCAGGATATGCACATCGCTGTGTTTGCCTCGATAATTGTAGCGGTGCCAGAATGGCAACCAGAATGTTATTGAACAACGG
CCATCAACGTATCGGATACCTTGCCTCCAGTCACGGCATTGAAGATGATGCGATGCGTAAAGCAGGCTGGCTGAGTGCGC
TAAAAGAGCGGGATATTGTTCCGCCAGATAGCTGGATTGGTATGGGAACGCCAGACATGCAAGGAGGGGAAGCCGCGATG
GTAGAGCTACTGGGGCGCAATTTGCAACTCTCCGCCGTGTTTGCGTATAACGACAATATGGCCGCAGGAGCGCTAACCGC
GTTAAAAGACAACGGCATTACCATTCCACAGCATCTTTCTATTATCGGTTTTGACGATATCCCTATCGCCCGATATACCG
ATCCACAACTGACCACCGTTCGTTATCCCATTGCATCGATGGCAAAAATGGCGACGGAACTGGCATTACAAGGGGCAGCG
GGTACGCTGGATCTTTCCGCCAGTCACTGCTTTATGCCCACTCTGGTTCGTCGTCATTCAGTTGCTGCCAGACAGAATGC
GGCAGCGATCACTAACTAA

Upstream 100 bases:

>100_bases
GATCACATTCATTAACAAAATGACTGTAACCGTTTCCATTGCTGTGACTCGATTCACGAAGTCCTGTATTCAGTGCTGAC
AAAATAGCCGCCAGCATGCA

Downstream 100 bases:

>100_bases
GCAAATCACCTGATGTAACCGCTTTCAATCTGTGAGTGATTTCACAGTATCTTAACAATGTGATGGCTATGATGGCACCG
TTTTCAGTGCTGTAACCGAT

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAYCNFQNRVVNMITIRDVARQAGVSVATVSRVLNNSVLVSPDTREAVMKAVTLLDYRPNANAQALATQISETIGVVVMD
VSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDEELVQFMEQIPGMVLINRLV
PGYAHRCVCLDNCSGARMATRMLLNNGHQRIGYLASSHGIEDDAMRKAGWLSALKERDIVPPDSWIGMGTPDMQGGEAAM
VELLGRNLQLSAVFAYNDNMAAGALTALKDNGITIPQHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKMATELALQGAA
GTLDLSASHCFMPTLVRRHSVAARQNAAAITN

Sequences:

>Translated_352_residues
MAYCNFQNRVVNMITIRDVARQAGVSVATVSRVLNNSVLVSPDTREAVMKAVTLLDYRPNANAQALATQISETIGVVVMD
VSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDEELVQFMEQIPGMVLINRLV
PGYAHRCVCLDNCSGARMATRMLLNNGHQRIGYLASSHGIEDDAMRKAGWLSALKERDIVPPDSWIGMGTPDMQGGEAAM
VELLGRNLQLSAVFAYNDNMAAGALTALKDNGITIPQHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKMATELALQGAA
GTLDLSASHCFMPTLVRRHSVAARQNAAAITN
>Mature_351_residues
AYCNFQNRVVNMITIRDVARQAGVSVATVSRVLNNSVLVSPDTREAVMKAVTLLDYRPNANAQALATQISETIGVVVMDV
SDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDEELVQFMEQIPGMVLINRLVP
GYAHRCVCLDNCSGARMATRMLLNNGHQRIGYLASSHGIEDDAMRKAGWLSALKERDIVPPDSWIGMGTPDMQGGEAAMV
ELLGRNLQLSAVFAYNDNMAAGALTALKDNGITIPQHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKMATELALQGAAG
TLDLSASHCFMPTLVRRHSVAARQNAAAITN

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=91.7647058823529, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=55.7575757575758, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=35.1190476190476, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=32.8267477203647, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=32.7327327327327, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=29.1803278688525, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=28.2539682539683, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786540, Length=345, Percent_Identity=27.8260869565217, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787906, Length=301, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=25.7328990228013, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.08038585209, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789456, Length=331, Percent_Identity=25.3776435045317, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790689, Length=292, Percent_Identity=25.3424657534247, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38300; Mature: 38168

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYCNFQNRVVNMITIRDVARQAGVSVATVSRVLNNSVLVSPDTREAVMKAVTLLDYRPN
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCC
ANAQALATQISETIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAI
CCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
EVLIRQRCNALIVHSKALSDEELVQFMEQIPGMVLINRLVPGYAHRCVCLDNCSGARMAT
HHHHHHHCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCHHHHH
RMLLNNGHQRIGYLASSHGIEDDAMRKAGWLSALKERDIVPPDSWIGMGTPDMQGGEAAM
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
VELLGRNLQLSAVFAYNDNMAAGALTALKDNGITIPQHLSIIGFDDIPIARYTDPQLTTV
HHHHCCCCEEEEEEEECCCCCHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
RYPIASMAKMATELALQGAAGTLDLSASHCFMPTLVRRHSVAARQNAAAITN
ECCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYCNFQNRVVNMITIRDVARQAGVSVATVSRVLNNSVLVSPDTREAVMKAVTLLDYRPN
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCC
ANAQALATQISETIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAI
CCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
EVLIRQRCNALIVHSKALSDEELVQFMEQIPGMVLINRLVPGYAHRCVCLDNCSGARMAT
HHHHHHHCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCHHHHH
RMLLNNGHQRIGYLASSHGIEDDAMRKAGWLSALKERDIVPPDSWIGMGTPDMQGGEAAM
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
VELLGRNLQLSAVFAYNDNMAAGALTALKDNGITIPQHLSIIGFDDIPIARYTDPQLTTV
HHHHCCCCEEEEEEEECCCCCHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
RYPIASMAKMATELALQGAAGTLDLSASHCFMPTLVRRHSVAARQNAAAITN
ECCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]