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The map label for this gene is wzb [H]

Identifier: 218549476

GI number: 218549476

Start: 2177093

End: 2177536

Strand: Reverse

Name: wzb [H]

Synonym: EFER_2145

Alternate gene names: 218549476

Gene position: 2177536-2177093 (Counterclockwise)

Preceding gene: 218549477

Following gene: 218549475

Centisome position: 47.45

GC content: 54.05

Gene sequence:

>444_bases
ATGTTTAACAACATCTTAGTTGTCTGTGTCGGCAATATTTGCCGTTCCCCGACGGCGGAACGCTTACTGCAACGTTATCA
CCCTGAGCTGAAAGTGGAGTCCGCTGGACTCGGTGCGCTGGTCGGTAAGGGCGCTGACCCTACCGCTATCAGCGTCGCCG
CAGAACATCAACTGTCTCTGGAAGGTCACTGTGCCCGTCAAATCAGCCGCAGCCTGTGTCGCAACTACGACCTGATTTTG
ACCATGGAAAAGCGCCATATCGAACGCTTATGCGAGATGGCACCTGAGATGCGCGGCAAAGTGATGCTGTTTGGTCACTG
GGATAACGAATGTGAAATCCCCGATCCGTATCGCAAAAGCCGGGAAACGTTTGCAGCGGTGTACACATTACTTGAACGGT
CTGCCCGCCAGTGGGCGCAGGCATTGAACGCAGAGCAGGTATAA

Upstream 100 bases:

>100_bases
TTTCCCGCTGGAACCGTCTGATCAATCAGTTGCTGCCAACTATTAGCGGTGTCCGTTACATGACGGATACAGCCAGCGAC
ATTCATAACTGGTAATCGTC

Downstream 100 bases:

>100_bases
CAATGACAGAAAAAGTAAAACAACATGCCGCTCCGGTAACGGGCAGTGATGAAATCGATATTGGTCGCCTGGTCGGCACC
GTCATTGAAGCGCGCTGGTG

Product: tyrosine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV

Sequences:

>Translated_147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV
>Mature_147_residues
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLIL
TMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV

Specific function: Dephosphorylates wzc. Required for the extracellular polysaccharide colanic acid synthesis. Probably involved in the export of colanic acid from the cell to medium [H]

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI6005988, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788375, Length=147, Percent_Identity=99.3197278911565, Blast_Score=305, Evalue=1e-84,
Organism=Escherichia coli, GI87081812, Length=142, Percent_Identity=56.3380281690141, Blast_Score=159, Evalue=7e-41,
Organism=Drosophila melanogaster, GI78706766, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706768, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706764, Length=127, Percent_Identity=33.0708661417323, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000106
- InterPro:   IPR017867 [H]

Pfam domain/function: PF01451 LMWPc [H]

EC number: =3.1.3.48 [H]

Molecular weight: Translated: 16640; Mature: 16640

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSL
CCCCEEEEEECHHHCCCHHHHHHHHHCCCCEECCCCCCHHHCCCCCCCEEEEEHHCCCCC
EGHCARQISRSLCRNYDLILTMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKS
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHH
RETFAAVYTLLERSARQWAQALNAEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKGADPTAISVAAEHQLSL
CCCCEEEEEECHHHCCCHHHHHHHHHCCCCEECCCCCCHHHCCCCCCCEEEEEHHCCCCC
EGHCARQISRSLCRNYDLILTMEKRHIERLCEMAPEMRGKVMLFGHWDNECEIPDPYRKS
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHH
RETFAAVYTLLERSARQWAQALNAEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]