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The map label for this gene is pduF [H]

Identifier: 218549340

GI number: 218549340

Start: 2060147

End: 2060941

Strand: Reverse

Name: pduF [H]

Synonym: EFER_2007

Alternate gene names: 218549340

Gene position: 2060941-2060147 (Counterclockwise)

Preceding gene: 218549360

Following gene: 218549339

Centisome position: 44.91

GC content: 50.19

Gene sequence:

>795_bases
ATGATGAAAGATTCACTGAAAGCGCAATGTATCGCCGAGTTTTTAGGCACCGGGCTATTTCTGTTTTTCGGCATTGGCTG
CGTTTGTGCAGCAAAACTTGCCGGGGCAAGCCTTGGGCTTTGGGAAATTTGTATTATTTGGGGGCTGGGGATTTCCCTGG
CGGTTTACCTGACAGCAGGGATCTCGGGTGCGCATTTAAATCCTGCGGTCACAATCGCATTGTGGTTATTTGCCCGTTTT
CCTGCGCATAAGGTGTTGCCATATTCGTTAGCGCAGTTTGCTGGCGCTTTTAGTGGAGCTGTGCTCGCATGGTTCCTGTA
CAGCAACCTTTTTGTCGAGTATGAAACGGCACACAATATGGTGCGTGGCAGCGTAGAAAGTCTGGCTGTTGCGGGTGTCT
TTAGTACTTACCCGGCGGCAGCGCTGAGTATCTGGCAGGCGTTGCTGGTGGAAATAGTCATCACATCTATCCTGATGGGC
TTAATCATGGCATTAACCGATGACGGTAACGGTGTGCCGAAAGGGCCGTTAGCGCCTTTGTTAATTGGTATTCTGGTTGC
GGTCATTGGTGCCTCCATGGGACCGTTGACAGGGTTTGCAATGAACCCGGCGCGTGACTTTGGTCCAAAACTGTTCGCAT
GGCTTGCCGGATGGGGCAATGTAGCGATGACAGGTGGCCGTGATATTCCCTATTTTATTGTGCCAATAATAGCTCCGGTA
ATAGGTGCCTGTGGCGGCGCTGCAATTTATCGTTATTTTATTGGTAACAACTTACCCACGAATGAATCGGTGTGA

Upstream 100 bases:

>100_bases
AAAATATTTCGCAGGTGAGCAACAAATTCATATGCAGGCGTTTCTATAGTAACGCCCGTAACTACGGTTAATCAGCGACA
GACTCAACCAGGAAGGTGTC

Downstream 100 bases:

>100_bases
TTTTAAGCTGTTAGCCATTTTTATAACGCTATTTCGTGATCTGCTTTCCCGAACTGAACAAAAAGTCTCCCCTCAAAAGG
AGACTTTTGTTATTGTCCGT

Product: Propanediol diffusion facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MMKDSLKAQCIAEFLGTGLFLFFGIGCVCAAKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFARF
PAHKVLPYSLAQFAGAFSGAVLAWFLYSNLFVEYETAHNMVRGSVESLAVAGVFSTYPAAALSIWQALLVEIVITSILMG
LIMALTDDGNGVPKGPLAPLLIGILVAVIGASMGPLTGFAMNPARDFGPKLFAWLAGWGNVAMTGGRDIPYFIVPIIAPV
IGACGGAAIYRYFIGNNLPTNESV

Sequences:

>Translated_264_residues
MMKDSLKAQCIAEFLGTGLFLFFGIGCVCAAKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFARF
PAHKVLPYSLAQFAGAFSGAVLAWFLYSNLFVEYETAHNMVRGSVESLAVAGVFSTYPAAALSIWQALLVEIVITSILMG
LIMALTDDGNGVPKGPLAPLLIGILVAVIGASMGPLTGFAMNPARDFGPKLFAWLAGWGNVAMTGGRDIPYFIVPIIAPV
IGACGGAAIYRYFIGNNLPTNESV
>Mature_264_residues
MMKDSLKAQCIAEFLGTGLFLFFGIGCVCAAKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFARF
PAHKVLPYSLAQFAGAFSGAVLAWFLYSNLFVEYETAHNMVRGSVESLAVAGVFSTYPAAALSIWQALLVEIVITSILMG
LIMALTDDGNGVPKGPLAPLLIGILVAVIGASMGPLTGFAMNPARDFGPKLFAWLAGWGNVAMTGGRDIPYFIVPIIAPV
IGACGGAAIYRYFIGNNLPTNESV

Specific function: May facilitate the diffusion of propanediol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=266, Percent_Identity=37.218045112782, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4826645, Length=268, Percent_Identity=37.6865671641791, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI4502187, Length=264, Percent_Identity=34.8484848484849, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI22538420, Length=260, Percent_Identity=35, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI310133356, Length=217, Percent_Identity=32.7188940092166, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI310114181, Length=217, Percent_Identity=32.7188940092166, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI45446752, Length=214, Percent_Identity=33.1775700934579, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502179, Length=244, Percent_Identity=34.4262295081967, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI86792455, Length=203, Percent_Identity=35.9605911330049, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502183, Length=261, Percent_Identity=32.183908045977, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI6912506, Length=201, Percent_Identity=31.8407960199005, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790362, Length=260, Percent_Identity=68.8461538461538, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1787101, Length=220, Percent_Identity=30.4545454545455, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=264, Percent_Identity=37.1212121212121, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71994009, Length=265, Percent_Identity=35.8490566037736, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17544068, Length=256, Percent_Identity=37.890625, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=259, Percent_Identity=33.2046332046332, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564052, Length=259, Percent_Identity=33.2046332046332, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=262, Percent_Identity=31.2977099236641, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=262, Percent_Identity=31.2977099236641, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=266, Percent_Identity=32.7067669172932, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71993722, Length=249, Percent_Identity=28.1124497991968, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=265, Percent_Identity=29.0566037735849, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI212646268, Length=231, Percent_Identity=26.8398268398268, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=38.6792452830189, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322985, Length=301, Percent_Identity=28.2392026578073, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45550503, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762344, Length=203, Percent_Identity=32.512315270936, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762346, Length=203, Percent_Identity=32.512315270936, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762348, Length=203, Percent_Identity=32.512315270936, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130305, Length=203, Percent_Identity=32.512315270936, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652747, Length=210, Percent_Identity=29.5238095238095, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551084, Length=210, Percent_Identity=29.5238095238095, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27679; Mature: 27679

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMKDSLKAQCIAEFLGTGLFLFFGIGCVCAAKLAGASLGLWEICIIWGLGISLAVYLTAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
ISGAHLNPAVTIALWLFARFPAHKVLPYSLAQFAGAFSGAVLAWFLYSNLFVEYETAHNM
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRGSVESLAVAGVFSTYPAAALSIWQALLVEIVITSILMGLIMALTDDGNGVPKGPLAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LIGILVAVIGASMGPLTGFAMNPARDFGPKLFAWLAGWGNVAMTGGRDIPYFIVPIIAPV
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHH
IGACGGAAIYRYFIGNNLPTNESV
HHCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8071226; 9352910; 11677609 [H]