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The map label for this gene is tpx [H]

Identifier: 218549002

GI number: 218549002

Start: 1697812

End: 1698318

Strand: Direct

Name: tpx [H]

Synonym: EFER_1650

Alternate gene names: 218549002

Gene position: 1697812-1698318 (Clockwise)

Preceding gene: 218549000

Following gene: 218549011

Centisome position: 37.0

GC content: 47.93

Gene sequence:

>507_bases
ATGTCACAAACCGTTCATTTCCAGGGCAACCCGGTTGCAGTCGCAAACTCCATTCCTCAGGCGGGTAGCAAAGCGCAGCC
TTTTACTCTCGTGGCAAAAGATCTGTCTGACGTCACACTGAGTCAGTTTGCTGGCAAACGCAAAGTGTTAAATATTTTCC
CAAGTATCGATACCGGCGTATGCGCAGCATCGGTACGTAAGTTTAATCAACTGGTAACAGAAATCGACAACACGGTTGTG
CTGTGCGTTTCTGCTGACCTGCCGTTCGCTCAGTCTCGTTTTTGCGGTGCAGAAGGTTTAAATAACGTCATCACCCTCTC
TACTTTCCGCAATGCTGAGTTCCTCAAAAATTATGGTGTAGAAATCACCGACGGCCCGCTGAAAGGTCTGGCAGCACGTG
CAGTTGTCGTTATTGACGAAAATGACAATGTTGTTTTCAGCCAGTTGGTAAATGAAATCACCACCGAACCGGATTACGAC
ACCGCACTCACTGTGCTGAAAGCGTAA

Upstream 100 bases:

>100_bases
TGCGATACCAAAAGATCAGGCGTAAATAGCGTTTGATGTGTTATAGACTGTTGGCATAAAATAGTGGTGACGTTAACTAT
ATGTAAATAGGAAGATAACC

Downstream 100 bases:

>100_bases
GAAGCGAAAAGCCTGATAAGCAACTCTGCTATCCTGCTATCAGGCTTTAACATTACTCTTCGCTTTTTTTCTGATTCAGA
CCATATTCACGCAATTTATT

Product: thiol peroxidase

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: Scavengase P20 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSQTVHFQGNPVAVANSIPQAGSKAQPFTLVAKDLSDVTLSQFAGKRKVLNIFPSIDTGVCAASVRKFNQLVTEIDNTVV
LCVSADLPFAQSRFCGAEGLNNVITLSTFRNAEFLKNYGVEITDGPLKGLAARAVVVIDENDNVVFSQLVNEITTEPDYD
TALTVLKA

Sequences:

>Translated_168_residues
MSQTVHFQGNPVAVANSIPQAGSKAQPFTLVAKDLSDVTLSQFAGKRKVLNIFPSIDTGVCAASVRKFNQLVTEIDNTVV
LCVSADLPFAQSRFCGAEGLNNVITLSTFRNAEFLKNYGVEITDGPLKGLAARAVVVIDENDNVVFSQLVNEITTEPDYD
TALTVLKA
>Mature_167_residues
SQTVHFQGNPVAVANSIPQAGSKAQPFTLVAKDLSDVTLSQFAGKRKVLNIFPSIDTGVCAASVRKFNQLVTEIDNTVVL
CVSADLPFAQSRFCGAEGLNNVITLSTFRNAEFLKNYGVEITDGPLKGLAARAVVVIDENDNVVFSQLVNEITTEPDYDT
ALTVLKA

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2) within the catalase- and peroxidase-deficient periplasmic space [H]

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1787584, Length=168, Percent_Identity=91.6666666666667, Blast_Score=316, Evalue=6e-88,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: 1.11.1.- [C]

Molecular weight: Translated: 18008; Mature: 17876

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVHFQGNPVAVANSIPQAGSKAQPFTLVAKDLSDVTLSQFAGKRKVLNIFPSIDTGV
CCCEEEECCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCHH
CAASVRKFNQLVTEIDNTVVLCVSADLPFAQSRFCGAEGLNNVITLSTFRNAEFLKNYGV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCC
EITDGPLKGLAARAVVVIDENDNVVFSQLVNEITTEPDYDTALTVLKA
EEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHEEEECC
>Mature Secondary Structure 
SQTVHFQGNPVAVANSIPQAGSKAQPFTLVAKDLSDVTLSQFAGKRKVLNIFPSIDTGV
CCEEEECCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCHH
CAASVRKFNQLVTEIDNTVVLCVSADLPFAQSRFCGAEGLNNVITLSTFRNAEFLKNYGV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCC
EITDGPLKGLAARAVVVIDENDNVVFSQLVNEITTEPDYDTALTVLKA
EEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]